Freshwater pulmonate snails from three locations in Lake Furesø north of Copenhagen were screened for infection with furcocercariae (by shedding in the laboratory) and recovered parasite larvae were diagnosed by molecular methods (by performing PCR of rDNA and sequencing the internal transcribed spacer [ITS] region). Overall prevalence of infection in snails was 2%. Recovered cercariae from Lymnaea stagnalis (Linnaeus) were diagnosed as Diplostomum pseudospathaceum Niewiadomska, 1984 (prevalence 4%) and cercariae from Radix balthica (Linnaeus) as D. mergi (Dubois, 1932) (prevalence 2%). Pathogen-free rainbow trout were then exposed to isolated cercariae and infection success and site location of metacercariae in these fish were determined. Infection experiments confirmed that both species could infect rainbow trout with the eye lens as infection site for the metacercarial stage although infection success differed. Combination of molecular and biological assays may contribute to improvement of our knowledge on diagnosis, distribution and biology of diplostomids in fish.

• MeSH
• ekologie MeSH
• fylogeneze MeSH
• hlemýždi parazitologie   MeSH
• infekce červy třídy Trematoda parazitologie   přenos   veterinární   MeSH
• jezera parazitologie   MeSH
• metacerkárie MeSH
• mezerníky ribozomální DNA chemie   genetika   MeSH
• molekulární sekvence - údaje MeSH
• nemoci ryb parazitologie   přenos   MeSH
• Oncorhynchus mykiss parazitologie   MeSH
• organismy bez specifických patogenů MeSH
• polymerázová řetězová reakce veterinární   MeSH
• roční období MeSH
• ryby MeSH
• sekvence nukleotidů MeSH
• sekvenční analýza DNA veterinární   MeSH
• sladká voda parazitologie   MeSH
• Trematoda genetika   izolace a purifikace   fyziologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Dánsko MeSH