Q124739639
Dotaz
Zobrazit nápovědu
AIM: The therapeutic potential of adipose-derived stem cell conditioned medium (ASC-CM) was studied in the rabbit model of critical limb ischemia (CLI). METHODS: Rabbits received treatment with ASC-CM or placebo. Gastrocnemius muscle tissue was collected 35 days after ischemia induction. Ischemic changes were evaluated in hematoxylin-eosin stained tissues for early (necrotic lesions/granulation tissue) and late (fibrous scars) phases of tissue repair. The expression of proangiogenic miR-126 was also evaluated using in situ hybridization. The levels of cytokines, insulin, and C-peptide were measured in blood. RESULTS: Early repair phases were observed more often in placebo-treated samples (45.5%) than in ASC-CM-treated ones (22.2%). However, the difference was not statistically significant. We demonstrated a statistically significant positive correlation between the early healing phases in tissue samples and C-peptide levels in peripheral blood. The expression of proangiogenic miR-126 was also shown in a number of structures in all phases of ischemic tissue healing. CONCLUSION: Based on our results, we believe that treatment with ASC-CM has the potential to accelerate the healing process in ischemic tissues in the rabbit model of CLI. The whole healing process was accompanied by miR-126 tissue expression. C-peptide could be used to monitor the course of the tissue healing process.
- MeSH
- C-peptid krev MeSH
- cytokiny krev MeSH
- diabetická noha MeSH
- dospělí MeSH
- experimentální diabetes mellitus krev MeSH
- fibróza MeSH
- fyziologická neovaskularizace účinky léků MeSH
- granulační tkáň patologie MeSH
- hojení ran účinky léků fyziologie MeSH
- hybridizace in situ MeSH
- inzulin krev MeSH
- ischemie krev MeSH
- jizva patologie MeSH
- kosterní svaly krevní zásobení účinky léků patologie MeSH
- králíci MeSH
- kultivační média speciální farmakologie MeSH
- lidé MeSH
- mezenchymální kmenové buňky * MeSH
- mikro RNA metabolismus MeSH
- modely nemocí na zvířatech MeSH
- nekróza MeSH
- zadní končetina MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- králíci MeSH
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Klíčová slova
- kontingenční tabulky,
- MeSH
- kontinuální vzdělávání * statistika a číselné údaje MeSH
- lidé MeSH
- neparametrická statistika MeSH
- personál nemocniční výchova MeSH
- postoj zdravotnického personálu MeSH
- pracovní uspokojení MeSH
- průzkumy a dotazníky MeSH
- řízení nemocnice MeSH
- rozdělení chí kvadrát MeSH
- statistika jako téma MeSH
- Check Tag
- lidé MeSH
BACKGROUND Paracrine factors secreted by adipose-derived stem cells can be captured, fractionated, and concentrated to produce therapeutic factor concentrate (TFC). The present study examined whether TFC effects could be enhanced by combining TFC with a biological matrix to provide sustained release of factors in the target region. MATERIAL AND METHODS Unilateral hind limb ischemia was induced in rabbits. Ischemic limbs were injected with either placebo control, TFC, micronized small intestinal submucosa tissue (SIS), or TFC absorbed to SIS. Blood flow in both limbs was assessed with laser Doppler perfusion imaging. Tissues harvested at Day 48 were assessed immunohistochemically for vessel density; in situ hybridization and quantitative real-time PCR were employed to determine miR-126 expression. RESULTS LDP ratios were significantly elevated, compared to placebo control, on day 28 in all treatment groups (p=0.0816, p=0.0543, p=0.0639, for groups 2-4, respectively) and on day 36 in the TFC group (p=0.0866). This effect correlated with capillary density in the SIS and TFC+SIS groups (p=0.0093 and p=0.0054, respectively, compared to placebo). A correlation was observed between miR-126 levels and LDP levels at 48 days in SIS and TFC+SIS groups. CONCLUSIONS A single bolus administration of TFC and SIS had early, transient effects on reperfusion and promotion of ischemia repair. The effects were not additive. We also discovered that TFC modulated miR-126 levels that were expressed in cell types other than endothelial cells. These data suggested that TFC, alone or in combination with SIS, may be a potent therapy for patients with CLI that are at risk of amputation.
- MeSH
- extracelulární matrix metabolismus MeSH
- ischemie genetika patologie terapie MeSH
- kmenové buňky cytologie MeSH
- končetiny krevní zásobení patologie MeSH
- králíci MeSH
- kůže patologie MeSH
- laser doppler flowmetrie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikro RNA genetika metabolismus MeSH
- mikropartikule metabolismus MeSH
- modely nemocí na zvířatech MeSH
- perfuze MeSH
- regulace genové exprese MeSH
- reperfuzní poškození patologie terapie MeSH
- střevní sliznice fyziologie MeSH
- tenké střevo fyziologie MeSH
- transplantace kmenových buněk * MeSH
- tuková tkáň cytologie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH