A limited number of studies are devoted to regulating TRIP6 expression in cancer. Hence, we aimed to unveil the regulation of TRIP6 expression in MCF-7 breast cancer cells (with high TRIP6 expression) and taxane-resistant MCF-7 sublines (manifesting even higher TRIP6 expression). We found that TRIP6 transcription is regulated primarily by the cyclic AMP response element (CRE) in hypomethylated proximal promoters in both taxane-sensitive and taxane-resistant MCF-7 cells. Furthermore, in taxane-resistant MCF-7 sublines, TRIP6 co-amplification with the neighboring ABCB1 gene, as witnessed by fluorescence in situ hybridization (FISH), led to TRIP6 overexpression. Ultimately, we found high TRIP6 mRNA levels in progesterone receptor-positive breast cancer and samples resected from premenopausal women.

• MeSH
• adaptorové proteiny signální transdukční genetika   MeSH
• AMP cyklický MeSH
• chemorezistence * genetika   MeSH
• hybridizace in situ fluorescenční MeSH
• lidé MeSH
• MFC-7 buňky MeSH
• nádory * genetika   MeSH
• P-glykoproteiny * genetika   MeSH
• proteiny s doménou LIM * genetika   MeSH
• responzivní elementy MeSH
• taxoidy MeSH
• transkripční faktory genetika   MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH

• MeSH
• teleradiologie metody   organizace a řízení   přístrojové vybavení   MeSH
• Publikační typ
• rozhovory MeSH

For chromosomal localization of the hFVIII human transgene in F2 and F3 generation of transgenic rabbits, FISH-TSA was applied. A short cDNA probe (1250 bp) targeted chromosomes 3, 7, 8, 9 and 18 of an F2 male (animal 1-3-8). Two transgenic offspring (F3) revealed signal positions in chromosome 3 and chromosomes 3 and 7, respectively. Sequencing and structure analysis of the rabbit orthologous gene revealed high similarity to its human counterpart. Part of the sequenced cDNA (1310 bp) served as a probe for FISH-TSA analysis. The rabbit gene was localized in the q arm terminus of the X chromosome. This result is in agreement with reciprocal chromosome painting between the rabbit and the human. The presented FISH-TSA method provides strong signals without any interspecies reactivity.

• MeSH
• faktor VIII genetika   metabolismus   MeSH
• geneticky modifikovaná zvířata MeSH
• genová dávka MeSH
• hybridizace in situ fluorescenční metody   MeSH
• králíci MeSH
• lidé MeSH
• mapování chromozomů metody   MeSH
• savčí chromozomy MeSH
• techniky amplifikace nukleových kyselin MeSH
• transgeny genetika   MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH

Two paralogous mitochondrial malate dehydrogenase 2 (Mdh2) genes of Xenopus laevis have been cloned and sequenced, revealing 95% identity. Fluorescence in-situ hybridization (FISH) combined with tyramide amplification discriminates both genes; Mdh2a was localized into chromosome q3 and Mdh2b into chromosome q8. One kb cDNA probes detect both genes with 85% accuracy. The remaining signals were on the paralogous counterpart. Introns interrupt coding sequences at the same nucleotide as defined for mouse. Restriction polymorphism has been detected in the first intron of Mdh2a, while the individual variability in intron 6 of Mdh2b gene is represented by an insertion of incomplete retrotransposon L1Xl. Rates of nucleotide substitutions indicate that both genes are under similar evolutionary constraints. X. laevis Mdh2 genes can be used as markers for physical mapping and linkage analysis.

• MeSH
• chromozomy MeSH
• duplicitní geny MeSH
• exprimované sekvenční adresy MeSH
• financování organizované MeSH
• genetická variace MeSH
• hybridizace in situ fluorescenční MeSH
• introny MeSH
• karyotypizace MeSH
• klonování DNA MeSH
• konzervovaná sekvence MeSH
• malátdehydrogenasa genetika   chemie   metabolismus   MeSH
• mapování chromozomů MeSH
• mitochondrie enzymologie   MeSH
• molekulární sekvence - údaje MeSH
• polymorfismus genetický MeSH
• retroelementy MeSH
• sekvence aminokyselin MeSH
• sekvence nukleotidů MeSH
• sekvenční homologie aminokyselin MeSH
• techniky amplifikace nukleových kyselin MeSH
• Xenopus laevis MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH