coherent diffractive imaging
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STUDY QUESTION: Can oocyte functionality be assessed by observing changes in their intracytoplasmic lipid droplets (LDs) profiles? SUMMARY ANSWER: Lipid profile changes can reliably be detected in human oocytes; lipid changes are linked with maternal age and impaired developmental competence in a mouse model. WHAT IS KNOWN ALREADY: In all cellular components, lipid damage is the earliest manifestation of oxidative stress (OS), which leads to a cascade of negative consequences for organelles and DNA. Lipid damage is marked by the accumulation of LDs. We hypothesized that impaired oocyte functionality resulting from aging and associated OS could be assessed by changes in LDs profile, hereafter called lipid fingerprint (LF). STUDY DESIGN, SIZE, DURATION: To investigate if it is possible to detect differences in oocyte LF, we subjected human GV-stage oocytes to spectroscopic examinations. For this, a total of 48 oocytes derived from 26 young healthy women (under 33 years of age) with no history of infertility, enrolled in an oocyte donation program, were analyzed. Furthermore, 30 GV human oocytes from 12 women were analyzed by transmission electron microscopy (TEM). To evaluate the effect of oocytes' lipid profile changes on embryo development, a total of 52 C57BL/6 wild-type mice and 125 Gnpat+/- mice were also used. PARTICIPANTS/MATERIALS, SETTING, METHODS: Human oocytes were assessed by label-free cell imaging via coherent anti-Stokes Raman spectroscopy (CARS). Further confirmation of LF changes was conducted using spontaneous Raman followed by Fourier transform infrared (FTIR) spectroscopies and TEM. Additionally, to evaluate whether LF changes are associated with developmental competence, mouse oocytes and blastocysts were evaluated using TEM and the lipid dyes BODIPY and Nile Red. Mouse embryonic exosomes were evaluated using flow cytometry, FTIR and FT-Raman spectroscopies. MAIN RESULTS AND THE ROLE OF CHANCE: Here we demonstrated progressive changes in the LF of oocytes associated with the woman's age consisting of increased LDs size, area, and number. LF variations in oocytes were detectable also within individual donors. This finding makes LF assessment a promising tool to grade oocytes of the same patient, based on their quality. We next demonstrated age-associated changes in oocytes reflected by lipid peroxidation and composition changes; the accumulation of carotenoids; and alterations of structural properties of lipid bilayers. Finally, using a mouse model, we showed that LF changes in oocytes are negatively associated with the secretion of embryonic exosomes prior to implantation. Deficient exosome secretion disrupts communication between the embryo and the uterus and thus may explain recurrent implantation failures in advanced-age patients. LIMITATIONS, REASONS FOR CAUTION: Due to differences in lipid content between different species' oocytes, the developmental impact of lipid oxidation and consequent LF changes may differ across mammalian oocytes. WIDER IMPLICATIONS OF THE FINDINGS: Our findings open the possibility to develop an innovative tool for oocyte assessment and highlight likely functional connections between oocyte LDs and embryonic exosome secretion. By recognizing the role of oocyte LF in shaping the embryo's ability to implant, our original work points to future directions of research relevant to developmental biology and reproductive medicine. STUDY FUNDING/COMPETING INTEREST(S): This research was funded by National Science Centre of Poland, Grants: 2021/41/B/NZ3/03507 and 2019/35/B/NZ4/03547 (to G.E.P.); 2022/44/C/NZ4/00076 (to M.F.H.) and 2019/35/N/NZ3/03213 (to Ł.G.). M.F.H. is a National Agency for Academic Exchange (NAWA) fellow (GA ULM/2019/1/00097/U/00001). K.F. is a Diamond Grant fellow (Ministry of Education and Science GA 0175/DIA/2019/28). The open-access publication of this article was funded by the Priority Research Area BioS under the program "Excellence Initiative - Research University" at the Jagiellonian University in Krakow. The authors declare no competing interest. TRIAL REGISTRATION NUMBER: N/A.
- MeSH
- dospělí MeSH
- embryonální vývoj fyziologie MeSH
- lidé MeSH
- lipidová tělíska metabolismus MeSH
- metabolismus lipidů MeSH
- myši inbrední C57BL * MeSH
- myši MeSH
- oocyty * metabolismus MeSH
- oxidační stres MeSH
- Ramanova spektroskopie MeSH
- stárnutí metabolismus MeSH
- transmisní elektronová mikroskopie MeSH
- věk matky MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
... Exert Combinatorial Control 520 -- An Incoherent Feed-forward Interaction Generates Pulses 522 -- A Coherent ... ... Are Seen Clearly in a Phase-Contrast or a -- Differential-Interference-Contrast Microscope 533 -- Images ... ... Total Internal Reflection -- Fluorescence Microscopy 547 -- Individual Molecules Can Be Touched, Imaged ... ... Different Views of a Single Object Can Be Combined to Give a Three-Dimensional Reconstruction 557 -- Images ... ... Cryoelectron Microscopy Both Allow -- Macromolecules to Be Viewed at High Resolution 559 -- Multiple Images ...
Sixth edition xxxiv, 1430 stran v různém stránkování : ilustrace (převážně barevné) ; 29 cm
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- molekulární biologie, molekulární medicína
- NLK Publikační typ
- učebnice vysokých škol
... space 455 -- 9 DIFFRACTION THEORY AND IMAGING 457 -- 9.1 WAVE EQUATIONS 458 -- 9.1.1 Maxwell’s equations ... ... Polychromatic, incoherent imaging 536 -- 9.7.8 Partially coherent imaging 537 -- 9.8 VOLUME DIFFRACTION ... ... diffraction from volume objects 545 -- 9.8.4 Coherent 3D imaging 547 -- 10 ENERGY TRANSPORT AND PHOTONS ... ... Artifacts 1215 -- 17.3.4 Image quality 1222 -- 18 COHERENT IMAGING AND SPECKLE 1235 -- 18.1 BASIC CONCEPTS ... ... fields 1291 -- 18.5.3 Univariate statistics of the image field and irradiance 1293 -- 18.6 COHERENT ...
Wiley series in pure and applied optics
[1st ed.] xli, 1540 s. : il.
... Physical Optics 3 -- Wave theory 3 -- Photon (particle) aspects of light 6 -- Interference and coherence ... ... 6 -- Applications of interference and coherence 7 -- Polarization 8 -- Applications of polarization ... ... 10 -- Diffraction 10 -- Scattering 12 -- Reflection 12 -- Transmission and absorption 14 -- Illumination ... ... Geometrical Optics 24 -- Pinhole imaging 24 -- Imaging with lenses and mirrors 25 -- Image characteristics ... ... 27 -- Transverse magnification 27 -- Image location 30 -- Depth of focus 31 -- Image quality 32 -- Brightness ...
xii, 304 stran : ilustrace ; 25 cm
- MeSH
- klinické lékařství MeSH
- kontaktní čočky MeSH
- oftalmologie MeSH
- optika a fotonika MeSH
- refrakce oka MeSH
- věda MeSH
- Konspekt
- Ortopedie. Chirurgie. Oftalmologie
- Učební osnovy. Vyučovací předměty. Učebnice
- NLK Obory
- oftalmologie
- věda a výzkum
- NLK Publikační typ
- učebnice vysokých škol
... Physical Optics 3 -- Wave theory 3 -- Photon (particle) aspects of light 6 -- Interference and coherence ... ... 6 -- Applications of interference and coherence 7 -- Polarization 8 -- Applications,.of polarization ... ... 10 -- Diffraction 10 -- Scattering 12 -- Reflection 12 -- Transmission and absorption 14 -- Illumination ... ... 24 -- Imaging with lenses and mirrors 25 -- Image characteristics 27 -- Transverse magnification 27 ... ... -- Image location 30 -- Depth of focus 31 -- Image quality 32 -- Brightness and irradiance 34 -- Miscellaneous ...
xii, 304 stran : ilustrace ; 25 cm
- MeSH
- klinické lékařství MeSH
- kontaktní čočky MeSH
- oftalmologie MeSH
- optika a fotonika MeSH
- refrakce oka MeSH
- věda MeSH
- Konspekt
- Učební osnovy. Vyučovací předměty. Učebnice
- Ortopedie. Chirurgie. Oftalmologie
- NLK Obory
- oftalmologie
- věda a výzkum
- NLK Publikační typ
- učebnice vysokých škol
... Physical Optics 26 -- Wave theory 26 -- Photon (particle) aspects of light 28 -- Interference 29 -- Coherence ... ... 29 -- Polarization 32 -- Diffraction 33 -- Scattering 34 -- Reflection 35 -- Transmission and absorption ... ... 41 -- Laser sources 44 -- Laser output 45 -- IL Geometrical Optics 55 -- The geometrical theory of image ... ... formation 55 -- Effects of pathology on image formation 56 -- Refractive index 58 -- Snell\'s law, total ... ... 72 -- Object-image movement 76 -- Virtual images and objects 76 -- Conjugate planes, transverse magnification ...
352 stran : ilustrace ; 25 cm
- MeSH
- klinické lékařství MeSH
- kontaktní čočky MeSH
- oftalmologie MeSH
- optika a fotonika MeSH
- refrakce oka MeSH
- věda MeSH
- Konspekt
- Ortopedie. Chirurgie. Oftalmologie
- Učební osnovy. Vyučovací předměty. Učebnice
- NLK Obory
- oftalmologie
- věda a výzkum
- NLK Publikační typ
- učebnice vysokých škol
... Coherence 21 -- E. Polarization 24 -- F. Diffraction 25 -- G. Scattering 26 -- H. ... ... Displacement of images by prisms 54 -- 4. Prismatic effect of lenses (Prentice\'s rule) 55 -- 5. ... ... Real vs. virtual objects and images 62 -- 7. Multiple lens systems 64 -- 8. ... ... Object-image movement 65 -- E. Graphical analysis of lens effects 65 -- 1. ... ... Reversal of the image space 95 -- 4. The central ray for mirrors 95 -- 5. ...
314 stran : ilustrace ; 25 cm
- MeSH
- klinické lékařství MeSH
- kontaktní čočky MeSH
- oftalmologie MeSH
- optika a fotonika MeSH
- refrakce oka MeSH
- věda MeSH
- Konspekt
- Ortopedie. Chirurgie. Oftalmologie
- Učební osnovy. Vyučovací předměty. Učebnice
- NLK Obory
- oftalmologie
- věda a výzkum
- NLK Publikační typ
- učebnice vysokých škol
... Coherence 21 -- E. Polarization 24 -- F. Diffraction 25 -- G. Scattering 26 -- H. ... ... Displacement of images by prisms 54 -- 4. Prismatic effect of lenses (Prentice\'s rule) 55 -- 5. ... ... Real vs. virtual objects and images 62 -- 7. Multiple lens systems 64 -- 8. ... ... Object-image movement 65 -- E. Graphical analysis of lens effects 65 -- 1. ... ... Reversal of the image space 95 -- 4. The central ray for mirrors 95 -- 5. ...
314 stran : ilustrace ; 25 cm
- MeSH
- klinické lékařství MeSH
- kontaktní čočky MeSH
- oftalmologie MeSH
- optika a fotonika MeSH
- refrakce oka MeSH
- věda MeSH
- Konspekt
- Ortopedie. Chirurgie. Oftalmologie
- Učební osnovy. Vyučovací předměty. Učebnice
- NLK Obory
- oftalmologie
- věda a výzkum
- NLK Publikační typ
- učebnice vysokých škol
... Coherence 21 -- E. Polarization 24 -- F. Diffraction 25 -- G. Scattering 26 -- H. ... ... Displacement of images by prisms 54 -- 4. Prismatic effect of lenses (Prentice\'s rule) 55 -- 5. ... ... Real vs. virtual objects and images 62 -- 7. Multiple lens systems 64 -- 8. ... ... Object-image movement 65 -- E. Graphical analysis of lens effects 65 -- 1. ... ... Reversal of the image space 95 -- 4. The central ray for mirrors 95 -- 5. ...
312 stran : ilustrace ; 25 cm
- MeSH
- klinické lékařství MeSH
- kontaktní čočky MeSH
- kurikulum MeSH
- oftalmologie MeSH
- optika a fotonika MeSH
- refrakce oka MeSH
- věda MeSH
- Konspekt
- Ortopedie. Chirurgie. Oftalmologie
- Učební osnovy. Vyučovací předměty. Učebnice
- NLK Obory
- věda a výzkum
- oftalmologie
- NLK Publikační typ
- učebnice vysokých škol
... Image formation in the electron microscope 83 -- 3.1 Elastic scattering 83 -- 3.2 Inelastic scattering ... ... 84 -- 3.3 Contrast 87 -- 3.4 Diffraction contrast 91 -- 3.5 Other methods of contrast improvement 96 ... ... -- 3.6 The formation of a Fresnel fringe 96 -- 3.7 Coherence 98 -- 3.8 The imaging of fine structure ... ... 110 -- 3.10.2 High dispersion electron diffraction 112 -- 3.10.3 Dark field microscopy 114 -- 3.10.4 ... ... of the image 277 -- 8.2.1 The effect of coherence 277 -- 8.2.2 The effect of focus setting 277 -- 8.2.3 ...
Practical methods in electron microscopy ; v. 2
Laboratory edition xii, 345 stran : ilustrace ; 23 cm
- MeSH
- elektronová mikroskopie MeSH
- fyzika MeSH
- mikroskopie MeSH
- Publikační typ
- monografie MeSH
- Konspekt
- Fyzika
- NLK Obory
- fyzika, biofyzika
