Patients with fixed orthodontic appliances tend to exhibit signs of gingivitis and gingival enlargement with false pockets. The aim of this study was to evaluate the occurrence of periodontal pathogens in patients with clinical manifestation of plaque-associated gingivitis treated with fixed orthodontic appliances. Thirty-two consecutive orthodontic patients – 11 females (age range from 10 to 32; mean age 17.43 years) and 21 males (age range from 12 to 28; average age 16.25 years) were included in this study. All individuals were diagnosed with clinical signs of gingivitis. CPI and PBI were evaluated. Subgingival plaque samples were collected after periodontal examination. Genomic DNA was extracted from these samples and bacterial detection was performed by polymerase chain reaction (Test VariOr-Dento). We recorded the following occurrence of periodontal pathogens: Actinobacillus actinomycetemcomitans – 25 %, Porphyromonas gingivalis – 31.25 %, Treponema denticola – 43.75 %, Tannerella forsythensis – 59 %, Prevotella intermedia – 65.6 %, Peptostreptococcus micros – 75 %, and Fusobacterium nucleatum – 100 %. Two and more pathogens were detected simultaneously in 93.75 % of the patients. Simultaneous occurrence of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis was recorded in 9.37 % of the patients. Likewise, simultaneous occurrence of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythensis was registered in 15.6 % of the patients. The number of Porphyromonas gingivalis bacteria in the sample correlated significantly (p=0.01) with the values of the CPI index (evaluated by the Kruskal-Wallis ANOVA test). In this study 80 % of the patients treated with the fixed orthodontic appliance and with clinical manifestations of plaqueinduced gingivitis are bearers of periodontal pathogens in a quantity significant for the damage of periodontium. To treat these patients successfully, collaboration with a periodontologist is highly recommended.

• MeSH
• Aggregatibacter actinomycetemcomitans izolace a purifikace   MeSH
• bakteriální infekce diagnóza   etiologie   komplikace   MeSH
• DNA bakterií izolace a purifikace   MeSH
• financování organizované MeSH
• Fusobacterium nucleatum izolace a purifikace   MeSH
• gingivitida diagnóza   etiologie   terapie   MeSH
• lidé MeSH
• ortodontické aparáty škodlivé účinky   MeSH
• Peptostreptococcus izolace a purifikace   MeSH
• polymerázová řetězová reakce metody   využití   MeSH
• Porphyromonas gingivalis izolace a purifikace   MeSH
• Prevotella intermedia izolace a purifikace   MeSH
• prospektivní studie MeSH
• statistika jako téma metody   MeSH
• Treponema denticola izolace a purifikace   MeSH
• zubní plak diagnóza   etiologie   komplikace   MeSH
• Check Tag
• lidé MeSH