• MeSH
• Aggregatibacter actinomycetemcomitans patogenita   MeSH
• antiinfekční látky terapeutické užití   MeSH
• biofilmy MeSH
• dítě MeSH
• dospělí MeSH
• implantace zubů MeSH
• lidé MeSH
• mladiství MeSH
• Papillonův-Lefevreův syndrom * komplikace   terapie   MeSH
• parodontitida komplikace   terapie   MeSH
• subgingivální kyretáž MeSH
• zubní implantáty MeSH
• zuby mléčné patologie   MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH

Periodontitis, an inflammatory disease caused by subgingival Gram-negative (G-) bacteria, is linked with loss of the connective tissue and destruction of the alveolar bone. In the regulation of inflammatory response, chemokine receptor 2 (CXCR2), a specific receptor for interleukin-8 and neutrophil chemoattractant, plays an important role. The first aim of this study was to investigate the CXCR2 gene variability in chronic periodontitis (CP) patients and healthy nonperiodontitis controls in the Czech population. The second aim was to find a relation between CXCR2 gene variants and the presence of periodontal bacteria. A total of 500 unrelated subjects participated in this case-control study. 329 CP patients and 171 healthy nonperiodontitis controls were analyzed using polymerase chain reaction techniques for three single-nucleotide polymorphisms (SNPs): +785C/T (rs2230054), +1208T/C (rs1126579), and +1440A/G (rs1126580). A DNA microarray detection kit was used for the investigation of the subgingival bacterial colonization, in a subgroup of CP subjects (N = 162). No significant differences in allele, genotype, haplotype, or haplogenotype frequencies of CXCR2 gene variants between patients with CP and healthy controls (P > 0.05) were determined. Nevertheless, Aggregatibacter actinomycetemcomitans was detected more frequently in men positive for the C allele of the CXCR2 +785C/T polymorphism (61.8% vs. 41.1%, P < 0.05; OR = 2.31, 95% CI = 1.03-5.20) and for the T allele of the CXCR2 +1208C/T variant (61.8% vs. 38.9%, P < 0.05; OR = 2.54, 95% CI = 1.13-5.71). In contrast, no statistically significant associations of CXCR2 variants with seven selected periodontal bacteria were found in women. Although none of the investigated SNPs in the CXCR2 gene was associated with CP, the CXCR2 gene variants can be associated with subgingival colonization of G- bacteria in men with CP in the Czech population.

• MeSH
• Aggregatibacter actinomycetemcomitans patogenita   MeSH
• alely MeSH
• chronická parodontitida genetika   mikrobiologie   MeSH
• dospělí MeSH
• genotyp MeSH
• haplotypy genetika   MeSH
• jednonukleotidový polymorfismus genetika   MeSH
• lidé středního věku MeSH
• lidé MeSH
• receptory interleukinu-8B genetika   MeSH
• studie případů a kontrol MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH

The colonization of the oral cavity by cariogenic and periodontal pathogens occurs earlier than previously thought. This study aimed to identify the presence and quantity of representative cariogenic and periodontal pathogens in the oral cavities of 6- and 12-month olds and to evaluate the influence of C-section delivery on early Streptococcus mutans (Sm) colonization of the oral cavity. The research cohort was composed of 59 infants (35 infants were delivered vaginally and 24 via C-section) and their mothers. At 6 months of age, the infants were examined, and unstimulated saliva samples were collected. Variables concerning mothers were DMF index and salivary levels of Sm. Repeated saliva samples were taken 6 months later. The representative cariogenic and periodontal microorganisms were identified, and their quantities were measured using a polymerase chain reaction-based method. The relationships between the presence of detected microbes, the mode of delivery, and maternal variables were evaluated using paired t tests, chi-squared test, and ANOVAs. High rates of cariogenic bacteria, Aggregatibacter actinomycetemcomitans (Aa) and Fusobacterium nucleatum (Fn), were found in both infant cohorts. An analysis of the differences between delivery methods revealed that the group of 6-month-old vaginally delivered infants had a significantly higher amount of Sm. We conclude that the cariogenic bacteria, Aa and Fn, are present in edentulous infants. This presence increases in the months following the eruption of the deciduous teeth. Results did not confirm the influence of C-section delivery on the early Sm colonization of the oral cavity.

• MeSH
• Aggregatibacter actinomycetemcomitans izolace a purifikace   MeSH
• bakteriální nálož MeSH
• Fusobacterium nucleatum izolace a purifikace   MeSH
• kojenec MeSH
• lidé MeSH
• mikrobiota * MeSH
• polymerázová řetězová reakce MeSH
• rizikové faktory MeSH
• sliny mikrobiologie   MeSH
• Streptococcus mutans izolace a purifikace   MeSH
• studie případů a kontrol MeSH
• ústa mikrobiologie   MeSH
• věkové faktory MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Chronic periodontitis (CP) is an inflammatory disease of the teeth-supporting tissues in which genetic predisposition, dental plaque bacteria, and immune mechanisms all play important roles. The aim of this study was to evaluate the occurrence of IL-4 gene polymorphisms in chronic periodontitis and to investigate the association between polymorphisms and cytokines production after bacterial stimulation. Sixty-two subjects (47 CP patients and 15 healthy controls) with detected two polymorphisms in the IL-4 gene (-590C/T and intron 3 VNTR) were examined. Production of cytokines (IL-1α, IL-1β, IL-4, IL-5, IL-6, IL-10, IL-17, TNFα, INFγ, and VEGF) was studied after in vitro stimulation of isolated peripheral blood by mitogens (Pokeweed mitogen, Concanavalin A), dental plaque bacteria (Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Porphyromonas gingivalis, and Prevotella intermedia), and Heat Shock Protein (HSP) 70 by the Luminex multiplex cytokine analysis system. The results were correlated with IL-4 genotypes in patients with CP and healthy controls. The mononuclear cells isolated from peripheral blood of CP patients with selected IL-4 polymorphisms significantly altered the production of IFNγ, IL-10, IL-1β, IL-1α, TNFα, and IL-6 after stimulation by HSP 70 or selected bacteria (from P < 0.001 to P < 0.05). IL-4 gene polymorphisms may influence the function of mononuclear cells to produce not only interleukin-4 but also other cytokines, especially in patients with CP.

• MeSH
• Aggregatibacter actinomycetemcomitans MeSH
• chronická nemoc MeSH
• cytokiny metabolismus   MeSH
• dospělí MeSH
• genotyp MeSH
• imunitní systém MeSH
• interleukin-4 genetika   metabolismus   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mitogeny chemie   MeSH
• parodontitida metabolismus   MeSH
• polymorfismus genetický * MeSH
• Porphyromonas gingivalis MeSH
• Prevotella intermedia MeSH
• sekvenční analýza DNA MeSH
• senioři MeSH
• studie případů a kontrol MeSH
• zánět MeSH
• zubní plak mikrobiologie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

BACKGROUND: Recently, the dental literature has focused mainly on the microbial colonization of healthy full-term infants and their mothers or caretakers. However, oral microbial acquisition by premature infants has not been adequately investigated, and the correlation between pre-term birth and the presence of cariogenic and periodontal pathogens has not been determined. The aim of this study was to identify the presence and quantities of representative cariogenic and periodontal pathogens in the oral cavities of 12-month-old infants and compare the occurrence of these microbes between a cohort of pre-term infants with very low birthweights and a control cohort comprising full-term infants. METHODS: The research cohort was composed of 69 one-year-old infants, of whom 24 were born prematurely with very low birthweights and 45 of whom were carried to full term. Information regarding the infants' gestational age, mode of delivery, general health status, birthweight and antibiotic use were obtained from hospital records and through oral interviews. At 12 months of age, both groups of infants were examined, and unstimulated saliva samples from the dorsum of the tongue and dental plaque samples were collected. The microorganisms (Streptococcus mutans, Lactobacillus spp., Actinomyces spp., Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Peptostreptococcus micros, Prevotella intermedia, Fusobacterium nucleatum) were identified and their quantities were evaluated using a PCR-based method. The chi-squared and Fisher's factorial tests were used for the statistical evaluations. RESULTS: The infants had a high prevalence of cariogenic microbes and of Fusosbacterium nucleatum and Aggregatibacter actinomycetemcomitans. Cariogenic microbes were detected in 91.7% of the very low birthweight infants and in all full-term infants. Periodontal pathogens were present in 83% of the pre-term infants and in 96% of the full-term infants. A significant difference was found between the cohorts in terms of the presence of S. mutans. Most of the very low birthweight infants had negative values of this microbe, while the full-term infants had positive values. CONCLUSIONS: This study confirms the early transmission of representative cariogenic and periodontal pathogens to the oral cavity of one-year-old infants and a higher prevalence of S. mutans in full-term infants than in premature infants.

• MeSH
• Actinomyces izolace a purifikace   MeSH
• Aggregatibacter actinomycetemcomitans izolace a purifikace   MeSH
• antibakteriální látky terapeutické užití   MeSH
• bakteriální nálož MeSH
• Fusobacterium nucleatum izolace a purifikace   MeSH
• gestační stáří MeSH
• jazyk mikrobiologie   MeSH
• kohortové studie MeSH
• kojenec MeSH
• Lactobacillus izolace a purifikace   MeSH
• lidé MeSH
• nemoci parodontu mikrobiologie   MeSH
• novorozenec nedonošený * MeSH
• novorozenec s nízkou porodní hmotností * MeSH
• novorozenec MeSH
• porod v termínu MeSH
• porodní hmotnost MeSH
• sliny mikrobiologie   MeSH
• Streptococcus mutans izolace a purifikace   MeSH
• studie případů a kontrol MeSH
• ústa mikrobiologie   MeSH
• vedení porodu MeSH
• zdravotní stav MeSH
• zubní kaz mikrobiologie   MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH
• novorozenec MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH

OBJECTIVES: Periodontitis is an inflammatory disease characterized by connective tissue loss and alveolar bone destruction. Interleukin-8 (IL8) is important in the regulation of the immune response. The aim of this study was to analyze four polymorphisms in the IL8 gene in relation to chronic (CP) and aggressive (AgP) periodontitis. METHODS: A total of 492 unrelated subjects were included in this case-control association study. Genomic DNA of 278 patients with CP, 58 patients with AgP, and 156 controls were genotyped, using the 5' nuclease TaqMan assay, for IL8 (rs4073, rs2227307, rs2227306, and rs2227532) gene polymorphisms. Subgingival bacterial colonization was investigated by the DNA-microarray detection kit in a subgroup of subjects (N = 247). RESULTS: Allele and genotype frequencies of all investigated IL8 polymorphisms were not significantly different between the subjects with CP and/or AgP and controls (P > 0.05). Nevertheless, the A(-251)/T(+396)/T(+781) and T(-251)/G(+396)/C(+781) haplotypes were significantly less frequent in patients with CP (2.0% versus 5.1%, P < 0.02, OR = 0.34, 95% CI: 0.15-0.78, resp., 2.0% versus 4.5%, P < 0.05, OR = 0.41, 95% CI: 0.18-0.97) than in controls. CONCLUSIONS: Although none of the investigated SNPs in the IL8 gene was individually associated with periodontitis, some haplotypes can be protective against CP in the Czech population.

• MeSH
• Aggregatibacter actinomycetemcomitans izolace a purifikace   MeSH
• agresivní parodontitida etiologie   genetika   mikrobiologie   MeSH
• dospělí MeSH
• haplotypy * MeSH
• interleukin-8 genetika   MeSH
• jednonukleotidový polymorfismus * MeSH
• lidé středního věku MeSH
• lidé MeSH
• studie případů a kontrol MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

I. Risk factors of atherosclerosis in patients with chronic periodontitis. II. Apo E-/- mice - role of commensal microflora in germfree and conventional conditions. III. Prague hereditary hypercholinesteromic rat and role of probiotics and dental plaquebacteria.

Anotace I: Úloha probiotik a bakterií zubního povlaku v rozvoji aterosklerózy Anotace III: Pacienti s chronickou parodontitidou a riziko aterosklerózy Anotace II: Experimentální modely aterosklerózy

• MeSH
• Aggregatibacter actinomycetemcomitans MeSH
• ateroskleróza MeSH
• cytokiny krev   MeSH
• modely u zvířat MeSH
• parodontitida MeSH
• Porphyromonas gingivalis MeSH
• potkani Wistar MeSH
• probiotika MeSH
• rizikové faktory MeSH
• zubní plak mikrobiologie   MeSH

• Konspekt
• Stomatologie
• NLK Obory
• zubní lékařství
• zubní lékařství
• NLK Publikační typ
• závěrečné zprávy o řešení grantu IGA MZ ČR

• MeSH
• Aggregatibacter actinomycetemcomitans patogenita   MeSH
• chronická nemoc terapie   MeSH
• parodontitida terapie   MeSH
• stomatochirurgické předprotetické výkony MeSH
• Publikační typ
• kazuistiky MeSH

Patients with fixed orthodontic appliances tend to exhibit signs of gingivitis and gingival enlargement with false pockets. The aim of this study was to evaluate the occurrence of periodontal pathogens in patients with clinical manifestation of plaque-associated gingivitis treated with fixed orthodontic appliances. Thirty-two consecutive orthodontic patients – 11 females (age range from 10 to 32; mean age 17.43 years) and 21 males (age range from 12 to 28; average age 16.25 years) were included in this study. All individuals were diagnosed with clinical signs of gingivitis. CPI and PBI were evaluated. Subgingival plaque samples were collected after periodontal examination. Genomic DNA was extracted from these samples and bacterial detection was performed by polymerase chain reaction (Test VariOr-Dento). We recorded the following occurrence of periodontal pathogens: Actinobacillus actinomycetemcomitans – 25 %, Porphyromonas gingivalis – 31.25 %, Treponema denticola – 43.75 %, Tannerella forsythensis – 59 %, Prevotella intermedia – 65.6 %, Peptostreptococcus micros – 75 %, and Fusobacterium nucleatum – 100 %. Two and more pathogens were detected simultaneously in 93.75 % of the patients. Simultaneous occurrence of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis was recorded in 9.37 % of the patients. Likewise, simultaneous occurrence of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythensis was registered in 15.6 % of the patients. The number of Porphyromonas gingivalis bacteria in the sample correlated significantly (p=0.01) with the values of the CPI index (evaluated by the Kruskal-Wallis ANOVA test). In this study 80 % of the patients treated with the fixed orthodontic appliance and with clinical manifestations of plaqueinduced gingivitis are bearers of periodontal pathogens in a quantity significant for the damage of periodontium. To treat these patients successfully, collaboration with a periodontologist is highly recommended.

• MeSH
• Aggregatibacter actinomycetemcomitans izolace a purifikace   MeSH
• bakteriální infekce diagnóza   etiologie   komplikace   MeSH
• DNA bakterií izolace a purifikace   MeSH
• financování organizované MeSH
• Fusobacterium nucleatum izolace a purifikace   MeSH
• gingivitida diagnóza   etiologie   terapie   MeSH
• lidé MeSH
• ortodontické aparáty škodlivé účinky   MeSH
• Peptostreptococcus izolace a purifikace   MeSH
• polymerázová řetězová reakce metody   využití   MeSH
• Porphyromonas gingivalis izolace a purifikace   MeSH
• Prevotella intermedia izolace a purifikace   MeSH
• prospektivní studie MeSH
• statistika jako téma metody   MeSH
• Treponema denticola izolace a purifikace   MeSH
• zubní plak diagnóza   etiologie   komplikace   MeSH
• Check Tag
• lidé MeSH

• MeSH
• Aggregatibacter actinomycetemcomitans patogenita   MeSH
• kardiovaskulární nemoci komplikace   patofyziologie   MeSH
• lidé MeSH
• parodontitida imunologie   komplikace   patofyziologie   MeSH
• Porphyromonas gingivalis patogenita   MeSH
• předčasný porod patofyziologie   MeSH
• preeklampsie patofyziologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• přehledy MeSH