Torque teno felis virus (FcTTV) was detected in the cat population in the Czech Republic. A total of 110 serum samples were tested by a nested PCR technique using specific primers, situated in the highly conserved untranslated region of the virus genome. The frequency of feline TT virus in the Czech Republic was found to be 33.63%. Sequencing of PCR product from several virus strains showed that all of them are closely related and belong to the same virus species. Whole genome sequencing of three strains was performed to compare overall genetic heterogeneity of feline TT viruses. One of these three strains showed more that 10% difference at the nucleotide level. Furthermore we didn't find any correlation between FcTTV infection and sex or health status of examined animals.
- MeSH
- fylogeneze MeSH
- genom virový genetika MeSH
- infekce DNA virem epidemiologie veterinární virologie MeSH
- kočky virologie MeSH
- nemoci koček epidemiologie virologie MeSH
- polymerázová řetězová reakce veterinární MeSH
- prevalence MeSH
- Torque teno virus genetika MeSH
- virové proteiny genetika MeSH
- zvířata MeSH
- Check Tag
- kočky virologie MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
The aim of study was to determine the influence of zinc chelate, valnemulin and it's combination on Brachyspira hyodysenteriae shedding and morphological changes of colonic mucosa in an experimental model of swine dysentery (SD). The study was performed on pigs coming from a dysentery-free herd. Animals were inoculated by B. hyodysenteriae strain B204. When the clinical signs of SD and B. hyodysenteriae shedding developed, the pigs were divided into four treatment groups. The first group was treated with zinc chelate (250 ml/1000 L in water), second group was given valnemulin in feed at 75 ppm; the third group was given a combination of both and the fourth group was control. The results demonstrated therapeutic effect of valnemulin in pigs with serious SD and did not show therapeutic effect of chelated zinc.
- MeSH
- Brachyspira hyodysenteriae růst a vývoj MeSH
- diterpeny terapeutické užití MeSH
- dyzenterie mikrobiologie patologie veterinární MeSH
- gramnegativní bakteriální infekce farmakoterapie mikrobiologie patologie veterinární MeSH
- imunohistochemie veterinární MeSH
- kolon patologie MeSH
- kombinovaná farmakoterapie veterinární MeSH
- nemoci prasat farmakoterapie mikrobiologie patologie MeSH
- prasata MeSH
- sefarosa analogy a deriváty terapeutické užití MeSH
- střevní sliznice patologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
AIMS: Aim of the study is to evaluate the use of recombinant Bhlp29.7 in immunoblotting with sera as a means to detect pig herds infected with Brachyspira hyodysenteriae. METHODS AND RESULTS: Sera samples from 789 sows and rectal swabs from 838 pigs of various categories on 22 farms of different size (median 450 animals), production type and history of swine dysentery (SD) were examined. Sera from 378 sows from farms with previous SD history were examined via immunoblotting. Specific antibodies were detected in 79 of these (20.9%). Examination of 411 serum samples from sows and gilts taken on 11 farms without previous history of SD detected specific antibodies in 13 sows and gilts (3.2%). These 13, however, had come from farms where the presence of B. hyodysenteriae was confirmed or SD status was not known. Seroprevalence in herds with previous SD history ranged from 2.5 to 35.7%. B. hyodysenteriae was confirmed on six (27.3%) of 22 monitored farms. CONCLUSIONS: Immunoblotting using recombinant antigen Bhlp29.7 in conjunction with culturing B. hyodysenteriae proved to be a valuable tool for detecting swine herds latently infected with B. hyodysenteriae. SIGNIFICANCE AND IMPACT OF THE STUDY: The use of immunoblotting with recombinant Bhlp29.7 should prove to be a useful adjunct to detecting herds with SD, and hence, it will assist in controlling this important disease.
- MeSH
- Brachyspira hyodysenteriae imunologie izolace a purifikace MeSH
- dyzenterie diagnóza imunologie veterinární MeSH
- gramnegativní bakteriální infekce diagnóza imunologie veterinární MeSH
- imunoblotting metody MeSH
- lidé MeSH
- lipoproteiny genetika imunologie MeSH
- nemoci prasat diagnóza imunologie mikrobiologie MeSH
- prasata MeSH
- proteiny vnější bakteriální membrány genetika imunologie MeSH
- protilátky bakteriální krev MeSH
- rekombinantní proteiny imunologie MeSH
- séroepidemiologické studie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
