The aim of study was to determine the influence of zinc chelate, valnemulin and it's combination on Brachyspira hyodysenteriae shedding and morphological changes of colonic mucosa in an experimental model of swine dysentery (SD). The study was performed on pigs coming from a dysentery-free herd. Animals were inoculated by B. hyodysenteriae strain B204. When the clinical signs of SD and B. hyodysenteriae shedding developed, the pigs were divided into four treatment groups. The first group was treated with zinc chelate (250 ml/1000 L in water), second group was given valnemulin in feed at 75 ppm; the third group was given a combination of both and the fourth group was control. The results demonstrated therapeutic effect of valnemulin in pigs with serious SD and did not show therapeutic effect of chelated zinc.
- MeSH
- Brachyspira hyodysenteriae růst a vývoj MeSH
- diterpeny terapeutické užití MeSH
- dyzenterie mikrobiologie patologie veterinární MeSH
- gramnegativní bakteriální infekce farmakoterapie mikrobiologie patologie veterinární MeSH
- imunohistochemie veterinární MeSH
- kolon patologie MeSH
- kombinovaná farmakoterapie veterinární MeSH
- nemoci prasat farmakoterapie mikrobiologie patologie MeSH
- prasata MeSH
- sefarosa analogy a deriváty terapeutické užití MeSH
- střevní sliznice patologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
AIMS: Aim of the study is to evaluate the use of recombinant Bhlp29.7 in immunoblotting with sera as a means to detect pig herds infected with Brachyspira hyodysenteriae. METHODS AND RESULTS: Sera samples from 789 sows and rectal swabs from 838 pigs of various categories on 22 farms of different size (median 450 animals), production type and history of swine dysentery (SD) were examined. Sera from 378 sows from farms with previous SD history were examined via immunoblotting. Specific antibodies were detected in 79 of these (20.9%). Examination of 411 serum samples from sows and gilts taken on 11 farms without previous history of SD detected specific antibodies in 13 sows and gilts (3.2%). These 13, however, had come from farms where the presence of B. hyodysenteriae was confirmed or SD status was not known. Seroprevalence in herds with previous SD history ranged from 2.5 to 35.7%. B. hyodysenteriae was confirmed on six (27.3%) of 22 monitored farms. CONCLUSIONS: Immunoblotting using recombinant antigen Bhlp29.7 in conjunction with culturing B. hyodysenteriae proved to be a valuable tool for detecting swine herds latently infected with B. hyodysenteriae. SIGNIFICANCE AND IMPACT OF THE STUDY: The use of immunoblotting with recombinant Bhlp29.7 should prove to be a useful adjunct to detecting herds with SD, and hence, it will assist in controlling this important disease.
- MeSH
- Brachyspira hyodysenteriae imunologie izolace a purifikace MeSH
- dyzenterie diagnóza imunologie veterinární MeSH
- gramnegativní bakteriální infekce diagnóza imunologie veterinární MeSH
- imunoblotting metody MeSH
- lidé MeSH
- lipoproteiny genetika imunologie MeSH
- nemoci prasat diagnóza imunologie mikrobiologie MeSH
- prasata MeSH
- proteiny vnější bakteriální membrány genetika imunologie MeSH
- protilátky bakteriální krev MeSH
- rekombinantní proteiny imunologie MeSH
- séroepidemiologické studie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
The aim of the present study was to evaluate the importance of clonal spread of Brachyspira hyodysenteriae resistant to pleuromutilins (tiamulin, valnemulin) on farms in the Czech Republic. Agar dilution method and macrorestriction fragment profile analysis by pulsed field gel electrophoresis were used to characterise 35 B hyodysenteriae isolates that were obtained from clinical cases of swine dysentery on 32 farms between 2000 and 2005. Most isolates showed multiple resistances to tiamulin, valnemulin, tylosin and lincomycin. A total of six pulsotypes were detected in these multiresistant isolates. An analysis of epidemiological data showed that multiresistant B hyodysenteriae isolates were more often detected on fattening farms (59 per cent), compared with farms with other types of production. Furthermore, it was found that multiresistant B hyodysenteriae clones were most frequently selected on farms with endemic incidence of swine dysentery. This finding was confirmed by the characterisation of 21 B hyodysenteriae isolates obtained from three large-scale operations in seven consecutive years.
- MeSH
- antibakteriální látky farmakologie MeSH
- Brachyspira hyodysenteriae účinky léků růst a vývoj patogenita MeSH
- dyzenterie farmakoterapie mikrobiologie veterinární MeSH
- gramnegativní bakteriální infekce farmakoterapie mikrobiologie přenos veterinární MeSH
- mikrobiální testy citlivosti veterinární MeSH
- mnohočetná bakteriální léková rezistence MeSH
- nemoci prasat farmakoterapie mikrobiologie přenos MeSH
- počet mikrobiálních kolonií veterinární MeSH
- prasata MeSH
- pulzní gelová elektroforéza veterinární MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
Single-chain antibodies (scFv) specific to Brachyspira hyodysenteriae were isolated from a phagemid library. Recombinant Bhlp 29.7 protein was used for scFv selection and individual clones were tested by ELISA and immunofluorescent test; four unique clones were isolated. One of selected clones was able to bind specifically B. hyodysenteriae in ELISA and immunofluorescence test. This is the first report of species-specific recombinant antibodies against B. hyodysenteriae.
- MeSH
- antigeny bakteriální imunologie MeSH
- bakteriální proteiny imunologie MeSH
- Brachyspira hyodysenteriae imunologie MeSH
- druhová specificita MeSH
- ELISA MeSH
- fluorescenční protilátková technika přímá MeSH
- gramnegativní bakteriální infekce diagnóza veterinární MeSH
- lipoproteiny imunologie MeSH
- monoklonální protilátky imunologie MeSH
- nemoci prasat diagnóza MeSH
- peptidová knihovna MeSH
- prasata MeSH
- protilátky bakteriální imunologie MeSH
- rekombinantní fúzní proteiny imunologie MeSH
- specificita protilátek MeSH
- variabilní oblast imunoglobulinu imunologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
