A balanced microbiota is a main prerequisite for the host's health. The aim of the present work was to develop defined pig microbiota (DPM) with the potential ability to protect piglets against infection with Salmonella Typhimurium, which causes enterocolitis. A total of 284 bacterial strains were isolated from the colon and fecal samples of wild and domestic pigs or piglets using selective and nonselective cultivation media. Isolates belonging to 47 species from 11 different genera were identified by MALDI-TOF mass spectrometry (MALDI-TOF MS). The bacterial strains for the DPM were selected for anti-Salmonella activity, ability to aggregate, adherence to epithelial cells, and to be bile and acid tolerant. The selected combination of 9 strains was identified by sequencing of the 16S rRNA gene as Bacillus sp., Bifidobacterium animalis subsp. lactis, B. porcinum, Clostridium sporogenes, Lactobacillus amylovorus, L. paracasei subsp. tolerans, Limosilactobacillus reuteri subsp. suis, and Limosilactobacillus reuteri (two strains) did not show mutual inhibition, and the mixture was stable under freezing for at least 6 months. Moreover, strains were classified as safe without pathogenic phenotype and resistance to antibiotics. Future experiments with Salmonella-infected piglets are needed to test the protective effect of the developed DPM.
- Publikační typ
- časopisecké články MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
Crohn's disease is a chronic immune-mediated intestinal inflammation targeted against a yet incompletely defined subset of commensal gut microbiota and occurs on the background of a genetic predisposition under the influence of environmental factors. Genome-wide association studies have identified about 70 genetic risk loci associated with Crohn's disease. The greatest risk for Crohn's disease represent polymorphisms affecting the CARD15 gene encoding nucleotide-binding oligomerization domain 2 (NOD2) which is an intracellular sensor for muramyl dipeptide, a peptidoglycan constituent of bacterial cell wall. The accumulated evidence suggests that gut microbiota represent an essential, perhaps a central factor in the induction and maintaining of Crohn's disease where dysregulation of normal co-evolved homeostatic relationships between intestinal microbiota and host mucosal immune system leads to intestinal inflammation. Taken together, these findings identify Crohn's disease as a syndrome of overlapping phenotypes that involves variable influences of genetic and environmental factors. A deeper understanding of different genetic abnormalities underlying Crohn's disease together with the identification of beneficial and harmful components of gut microbiota and their interactions are essential conditions for the categorization of Crohn's disease patients, which enable us to design more effective, preferably causative, individually tailored therapy.
- MeSH
- celogenomová asociační studie MeSH
- Crohnova nemoc genetika MeSH
- gastrointestinální trakt mikrobiologie MeSH
- genetická predispozice k nemoci MeSH
- interakce genů a prostředí MeSH
- lidé MeSH
- mikrobiota * MeSH
- polymorfismus genetický MeSH
- signální adaptorový protein Nod2 genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
Cíl studie: Diagnostika sepse u imunokompromitovaných pacientů je obtížná pro jejich modifikovanou reakci na infekci. Cílem experimentu bylo porovnání časné klinické a laboratorní odpovědi na sepsi mezi experimentální skupinou septických miniprasat s imunosupresí a bez ní. Typ studie: Experimentální, srovnávací. Materiál a metoda: Experiment byl prováděn na miniprasatech. Sepse byla vyvolána užitím modelu cekální ligace a punkce (CLP). Miniprasata se shodnými vstupními parametry byla randomizována do 3 skupin, skupiny sepse (n = 10), skupiny imunosuprese (n = 11) s podáváním trojkombinace imunosupresivních léků (cyklosporin, metylprednisolon, mykofenolát mofetil) před operací a kontrolní skupiny (n = 6). Po celou dobu experimentu byly v tříhodinových intervalech monitorovány plazmatické hladiny vybraných cytokinů. Výsledky: U všech CLP zvířat došlo k rozvoji septického šoku s teplotou a hemodynamickou odezvou. Kinetika plazmatických koncentrací TNFα, IL-1β, interferonu-γ a CRP byla podobná ve skupině zvířat se sepsí i s imunosupresí. U imunosuprimovaných zvířat byly hodnoty IL-4 signifikantně nižší po celou dobu sledování, naopak u stejné skupiny byly hodnoty IL-8 zvýšené. Deset hodin po operaci byly hodnoty IL-6 významně vyšší ve skupině se sepsí než ve skupině s imunosupresí. Byl nalezen signifikantně vyšší vzestup hladin IL-10 v 19., 22., a 25. hodině po operaci ve skupině imunosuprimovaných zvířat ve srovnání se skupinou bez imunosuprese. Závěr: V našem experimentu jsme zjistili signifikantní rozdíly v cytokinové odpovědi na experimentálně navozenou sepsi mezi skupinami imunokompetentních a imunosuprimovaných zvířat.
Objective:The diagnosis of sepsis in immunocompromised patients is difficult due to their modified response to infection. Our experiment was designed to compare the early clinical and laboratory response to sepsis between experimental groups of septic minipigs with and without immunosuppression. Design: Experimental, comparative study. Material and methods: Experiment was performed on minipigs. Sepsis was induced using a model of caecal ligation and puncture (CLP). Minipigs (with identical baseline parameters) were randomized into 3 group; the sepsis group (n = 10), the immunosuppression group (n = 11), which received immunosuppressive drugs (cyclosporine, methylprednisolone, mycophenolate mofetil) before surgery, and the sham group (n = 6). Plasmatic levels of selected cytokines throughout the experiment in three-hour interval were monitored. Results: All CLP animals developed septic shock with febrile and hemodynamic response. Also the kinetics of the plasma levels of TNFα, IL-1β, interferon-γ and CRP in both experimental groups with and without immunosuppression was similar. In immunosuppressed animals the levels of IL-4 were significantly lower in all time periods observed, on the contrary a significant increase of IL-8 levels in the same experimental group was found as well. Ten hours after surgery, significantly higher level of IL-6 was found in the sepsis group as compared to the immunosuppression group. There was a significantly greater increase in levels of IL-10, 19, 22 and 25 hours after surgery in immunosuppressed animals compared to the group without immunosuppression. Conclusion: We found significant differencies in cytokine response to experimental sepsis between the groups of immunocompetent and immunosuppressed animals in our experiment.
- Klíčová slova
- cytokin, cekální ligace a punkce, miniprase,
- MeSH
- biologické markery krev metabolismus MeSH
- břišní dutina patologie MeSH
- cyklosporin aplikace a dávkování MeSH
- cytokiny krev MeSH
- imunosupresiva škodlivé účinky terapeutické užití MeSH
- imunosupresivní léčba škodlivé účinky MeSH
- klinické zkoušky jako téma MeSH
- miniaturní prasata MeSH
- sepse diagnóza etiologie imunologie MeSH
- septický šok etiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Klíčová slova
- experimentální sepse,
- MeSH
- biologické markery krev MeSH
- biopsie MeSH
- břišní dutina patologie MeSH
- cékum chirurgie patofyziologie patologie MeSH
- feces MeSH
- histologické techniky MeSH
- laparotomie MeSH
- ligace MeSH
- miniaturní prasata MeSH
- modely u zvířat MeSH
- monitorování fyziologických funkcí statistika a číselné údaje MeSH
- perforace střeva patologie MeSH
- peritonitida etiologie MeSH
- sepse diagnóza etiologie patologie MeSH
- septický šok etiologie MeSH
- studie případů a kontrol MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
