The impact of probiotic supplementation of canine-derived strain Lactobacillus fermentum AD1-CCM7421 in freeze-dried form on quantitative composition of microbiota and short-chain fatty acid profile in feces of dogs was demonstrated by two independent studies (straightforward repeated-measures model; study I: a dose of 2 g per dog for 2 weeks, 10(8) CFU/g, n = 12; study II: 1 g per dog for 1 week, 10(7) CFU/g, n = 11. The results revealed a significant increase of lactic acid bacteria population persisting also after the cessation of probiotic application in both studies. A reduction of clostridia (study I, p (sum) < 0.01) and tested Gram-negative bacterial genera (coliforms, Aeromonas sp., Pseudomonas sp., study II, p < 0.05) was also detected. The strain AD1-CCM7421 colonized the canine digestive tract in sufficient numbers (10(5)-10(6) CFU/g) and it persisted in the majority of dogs after cessation of probiotic application. An increase of short-chain fatty acid concentrations (study I: butyric, succinic, valeric, formic acid) especially in the early post-treatment phase (p < 0.05) most likely led to a decrease of fecal pH value (p < 0.05) without negative influence on fecal consistency throughout the studies.
- MeSH
- Bacteria klasifikace genetika izolace a purifikace MeSH
- feces chemie mikrobiologie MeSH
- gastrointestinální trakt metabolismus mikrobiologie MeSH
- Limosilactobacillus fermentum chemie fyziologie MeSH
- lyofilizace MeSH
- mastné kyseliny metabolismus MeSH
- metagenom MeSH
- nemoci psů metabolismus mikrobiologie prevence a kontrola MeSH
- potravní doplňky analýza MeSH
- probiotika chemie MeSH
- psi MeSH
- zvířata MeSH
- Check Tag
- psi MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- dieta MeSH
- gnotobiologické modely fyziologie MeSH
- mikroklky fyziologie patologie MeSH
- prasata růst a vývoj MeSH
- tenké střevo růst a vývoj MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- gnotobiologické modely MeSH
- jejunum fyziologie mikrobiologie MeSH
- Lactobacillus casei MeSH
- prasata fyziologie mikrobiologie MeSH
- skot MeSH
- střevní sliznice MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH