We investigated the genetic basis of glycopeptide resistance in laboratory-derived strains of S. haemolyticus with emphasis on differences between vancomycin and teicoplanin. The genomes of two stable teicoplanin-resistant laboratory mutants selected on vancomycin or teicoplanin were sequenced and compared to parental S. haemolyticus strain W2/124. Only the two non-synonymous mutations, VraS Q289K and WalK V550L were identified. No other mutations or genome rearrangements were detected. Increased cell wall thickness, resistance to lysostaphin-induced lysis and adaptation of cell growth rates specifically to teicoplanin were phenotypes observed in a sequenced strain with the VraS Q289K mutation. Neither of the VraS Q289K and WalK V550L mutations was present in the genomes of 121S. haemolyticus clinical isolates. However, all but two of the teicoplanin resistant strains carried non-synonymous SNPs in vraSRTU and walKR-YycHIJ operons pointing to their importance for the glycopeptide resistance.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální léková rezistence genetika MeSH
- DNA bakterií genetika MeSH
- fenotyp MeSH
- genom bakteriální genetika MeSH
- histidinkinasa genetika MeSH
- jednonukleotidový polymorfismus genetika MeSH
- lidé MeSH
- rezistence na vankomycin genetika MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA MeSH
- stafylokokové infekce mikrobiologie MeSH
- Staphylococcus haemolyticus účinky léků genetika izolace a purifikace MeSH
- teikoplanin farmakologie MeSH
- vankomycin farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Polsko MeSH
Polymerase chain reaction (PCR) amplification of antibiotic resistance genes as well as staphylococcal cassette chromosome mec (SCCmec) typing and pulsed-field gel electrophoresis (PFGE) of SmaI macrorestriction fragments of genomic DNA were used to characterize 45 methicillin-resistant coagulase-negative staphylococci (MRCoNS) isolates responsible of bacteremia recovered in patients at the Bone Marrow Transplant Centre of Tunisia in 1998-2007. Among the 45 MRCoNS isolates, Staphylococcus epidermidis was the most prevalent species (75.6%) followed by Staphylococcus haemolyticus (22.2%) and Staphylococcus hominis (2.2%). Extended susceptibility profiles were generated for MRCoNS against 16 antimicrobial agents. Out of 45 mecA-positive strains, 43 (95.6%) were phenotypically methicillin-resistant and two (4.4%) were methicillin-susceptible. The msr(A) was the most prevalent gene (13 isolates; 48.1%) among erythromycin-resistant isolates. The erm(C) was found alone in seven (25.9%) or in combination with both erm(A) and erm(B) in two (7.4%) isolates. The aac(6')-Ie-aph(2″)-Ia was the most prevalent gene among aminoglycoside-resistant isolates, detected alone in 14 isolates (33.3%) isolates, in combination with ant(4')-Ia in 18 (42.8%) isolates, in combination with aph(3')-IIIa in four (9.5%) or with both ant(4')-Ia and aph(3')-IIIa in two (4.7%) isolates. The ant(4')-Ia was detected in three (7.1%) isolates and the aph(3')-IIIa in one (2.4%) isolate. Among tetracycline-resistant isolates, six (85.7%) strains harbored the tet(K) gene and one (14.3%) strain carried tet(K) and tet(M) genes. SCCmec types IV (31%) and III (24.5%), the most prevalent types detected, were found to be more resistant to non-β-lactam antibiotics. A wide diversity of isolates was observed by PFGE among MRCoNS.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální geny MeSH
- bakteriální léková rezistence MeSH
- bakteriemie mikrobiologie MeSH
- DNA bakterií genetika metabolismus MeSH
- hematologické nádory komplikace MeSH
- koagulasa sekrece MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- molekulární typizace MeSH
- polymerázová řetězová reakce metody MeSH
- pulzní gelová elektroforéza MeSH
- restrikční endonukleasy typu II metabolismus MeSH
- rezistence na methicilin MeSH
- stafylokokové infekce mikrobiologie MeSH
- Staphylococcus epidermidis klasifikace účinky léků genetika izolace a purifikace MeSH
- Staphylococcus haemolyticus klasifikace účinky léků genetika izolace a purifikace MeSH
- Staphylococcus hominis klasifikace účinky léků genetika izolace a purifikace MeSH
- techniky typizace bakterií MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Tunisko MeSH
The Staphylococcus strains acquired from scrapings from hospital environments were identified to the species level based on their biochemical properties. From the monitored sample the Staphylococcus epidermidis strains were selected for more accurate typing and tested on their virulence factor and ribotyped. The biotyping of S. epidermidis did not show any considerable intraspecific variation of these isolates and there were no atypical reactions, with the exception of three strains (out of 33). In contrast, the results of ribotyping showed greater heterogeneity of strains and unequivocally demonstrated the relation between the ribotype and the place of sample drawing. In addition to this fact, the found ribotypes repeat in the same environment in the long-term which suggests the occurrence and persistence of the same strains of conditionally pathogenic bacteria in hospital environment. We showed that ribotyping is a suitable method for precise and reliable detection of some coagulase-negative staphylococci.
- MeSH
- fenotyp MeSH
- finanční podpora výzkumu jako téma MeSH
- infekce spojené se zdravotní péčí etiologie genetika mikrobiologie MeSH
- koagulasa antagonisté a inhibitory izolace a purifikace MeSH
- lidé MeSH
- ribotypizace metody využití MeSH
- Staphylococcus epidermidis cytologie genetika izolace a purifikace MeSH
- Staphylococcus haemolyticus genetika izolace a purifikace MeSH
- Staphylococcus hominis cytologie genetika izolace a purifikace MeSH
- techniky typizace bakterií metody využití MeSH
- Check Tag
- lidé MeSH
- MeSH
- Aeromonas hydrophila izolace a purifikace MeSH
- bakteriologické techniky MeSH
- Bordetella pertussis izolace a purifikace MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- Neisseria lactamica izolace a purifikace MeSH
- Staphylococcus haemolyticus izolace a purifikace MeSH
- testování odbornosti laboratoří * klasifikace metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- grafy a diagramy MeSH
- komentáře MeSH
- tabulky MeSH
