- MeSH
- kazuistiky jako téma MeSH
- kritický stav ošetřování MeSH
- lidé MeSH
- metabolismus MeSH
- novorozenec MeSH
- novorozenecká sepse * diagnóza etiologie komplikace MeSH
- parenterální výživa * metody MeSH
- Staphylococcus epidermidis patogenita MeSH
- živiny aplikace a dávkování klasifikace MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- novorozenec MeSH
- Publikační typ
- přehledy MeSH
Východiská: Prevádzky pedikúr patria medzi zariadenia starostlivosti o ľudské telo, ktoré sa z hľadiska prenosu infekčných ochorení zaraďujú k rizikovým ľudským činnostiam. Dôvodom je manipulácia s rôznorodými pomôckami, ktorých nedostatočná dezinfekcia môže viesť k vzniku infekcií u klientov pedikúr. Ciele: Testovať účinnosť vykonávanej dezinfekcie pracovných prístrojov, nástrojov a povrchov v zariadeniach pedikúr sterovou metódou. Súbor a metodika: Bolo zrealizovaných 100 odberov v 10 prevádzkach pedikúr v Banskej Bystrici. Analýza bola zameraná len na bakteriálne, nie na mykotické či parazitárne infekcie. Výsledky: Zo základného súboru bolo identifikovaných 43 vzoriek (43%) ako pozitívnych na prítomnosť mikroorganizmov a 57 vzoriek (57%) ako negatívnych. Zo všetkých vzoriek bolo 31 vzoriek (31%) pozitívnych na Staphylococcus epidermidis, 5 vzoriek (5%) pozitívnych na aeróbne sporuláty, 4 vzorky (4%) pozitívne na Escherichia coli, 2 vzorky (2%) pozitívne na Pseudomonas aeruginosa, 1 vzorka (1%) pozitívna na Pseudomonas spp., 1 vzorka (1%) pozitívna na viridujúce streptokoky. Záver: Zisťovanie mikrobiologickej kontaminácie pomocou sterovej metódy a použité postupy mikrobiologickej identifikácie majú svoje opodstatnenie. Objektívne preukazujú dostatočnosť alebo nedostatočnosť vykonávanej dekontaminácie, čo je podkladom pre prijímanie a realizáciu nápravných opatrení v prevádzkach pedikúr.
Background: Pedicures belong to facilities providing care for human body. Considering occurring transmission of infectious diseases these are classified as risky human activities. The reason is manipulation with various aids, which if not properly sanitized, may lead to the onset of infections in pedicure clients. Aims: To test efficiency of performed disinfection of working instruments, devices and surfaces in pedicures applying swab method.File and Methodology: 100 samples from 10 facilities in Banská Bystrica were taken. The analysis focused only on bacterial contamination, excluding mycotic or parasitic infections. Results: In the file were 43 samples (43%) identified positive for the presence of microorganisms, 57 samples (57%) were negative. Out of all taken samples, in 31 samples (31%) were identified Staphylococcus epidermidis, 5 samples (5%) contained aerobic sporulates, 4 samples (4%) contained Escherichia coli, 2 samples (2%) contained Pseudomonas aeruginosa, 1 sample (1%) was positive for Pseudomonas spp., and 1 sample (1%) contained viridating streptococci. Conclusion: The work has proven the high usefulness of swab method and other applied practices, as these can objectively prove either sufficient or insufficient decontamination performed in the pedicures. This is the basis for performance of suitable corrective or preventive actions.
- Klíčová slova
- pedikúra,
- MeSH
- dekontaminace metody statistika a číselné údaje MeSH
- dezinfekce metody statistika a číselné údaje MeSH
- Escherichia coli patogenita MeSH
- infekční nemoci klasifikace mikrobiologie přenos MeSH
- kontaminace zdravotnického vybavení * prevence a kontrola statistika a číselné údaje MeSH
- lidé MeSH
- mikrobiologické techniky klasifikace metody MeSH
- Pseudomonas aeruginosa patogenita MeSH
- Staphylococcus epidermidis patogenita MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
- Geografické názvy
- Slovenská republika MeSH
Východiská: Prevádzky pedikúr patria medzi zariadenia starostlivosti o ľudské telo, ktoré sa z hľadiska prenosu infekčných ochorení zaraďujú k rizikovým ľudským činnostiam. Dôvodom je manipulácia s rôznorodými pomôckami, ktorých nedostatočná dezinfekcia môže viesť k vzniku infekcií u klientov pedikúr. Ciele: Testovať účinnosť vykonávanej dezinfekcie pracovných prístrojov, nástrojov a povrchov v zariadeniach pedikúr sterovou metódou. Súbor a metodika: Bolo zrealizovaných 100 odberov v 10 prevádzkach pedikúr v Banskej Bystrici. Analýza bola zameraná len na bakteriálne, nie na mykotické či parazitárne infekcie. Výsledky: Zo základného súboru bolo identifikovaných 43 vzoriek (43%) ako pozitívnych na prítomnosť mikroorganizmov a 57 vzoriek (57%) ako negatívnych. Zo všetkých vzoriek bolo 31 vzoriek (31%) pozitívnych na Staphylococcus epidermidis, 5 vzoriek (5%) pozitívnych na aeróbne sporuláty, 4 vzorky (4%) pozitívne na Escherichia coli, 2 vzorky (2%) pozitívne na Pseudomonas aeruginosa, 1 vzorka (1%) pozitívna na Pseudomonas spp., 1 vzorka (1%) pozitívna na viridujúce streptokoky. Záver: Zisťovanie mikrobiologickej kontaminácie pomocou sterovej metódy a použité postupy mikrobiologickej identifikácie majú svoje opodstatnenie. Objektívne preukazujú dostatočnosť alebo nedostatočnosť vykonávanej dekontaminácie, čo je podkladom pre prijímanie a realizáciu nápravných opatrení v prevádzkach pedikúr.
Background: Pedicures belong to facilities providing care for human body. Considering occurring transmission of infectious diseases these are classified as risky human activities. The reason is manipulation with various aids, which if not properly sanitized, may lead to the onset of infections in pedicure clients. Aims: To test efficiency of performed disinfection of working instruments, devices and surfaces in pedicures applying swab method.File and Methodology: 100 samples from 10 facilities in Banská Bystrica were taken. The analysis focused only on bacterial contamination, excluding mycotic or parasitic infections. Results: In the file were 43 samples (43%) identified positive for the presence of microorganisms, 57 samples (57%) were negative. Out of all taken samples, in 31 samples (31%) were identified Staphylococcus epidermidis, 5 samples (5%) contained aerobic sporulates, 4 samples (4%) contained Escherichia coli, 2 samples (2%) contained Pseudomonas aeruginosa, 1 sample (1%) was positive for Pseudomonas spp., and 1 sample (1%) contained viridating streptococci. Conclusion: The work has proven the high usefulness of swab method and other applied practices, as these can objectively prove either sufficient or insufficient decontamination performed in the pedicures. This is the basis for performance of suitable corrective or preventive actions.
- Klíčová slova
- pedikúra,
- MeSH
- dekontaminace metody statistika a číselné údaje MeSH
- dezinfekce metody statistika a číselné údaje MeSH
- Escherichia coli patogenita MeSH
- infekční nemoci klasifikace mikrobiologie přenos MeSH
- kontaminace zdravotnického vybavení * prevence a kontrola statistika a číselné údaje MeSH
- mikrobiologické techniky klasifikace metody MeSH
- Pseudomonas aeruginosa patogenita MeSH
- Staphylococcus epidermidis patogenita MeSH
- Publikační typ
- přehledy MeSH
- Geografické názvy
- Slovenská republika MeSH
- MeSH
- acne vulgaris * mikrobiologie terapie MeSH
- antibakteriální látky terapeutické užití MeSH
- lidé MeSH
- mikrobiální interakce MeSH
- mikrobiota * MeSH
- Propionibacterium acnes patogenita MeSH
- Staphylococcus epidermidis patogenita MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- novinové články MeSH
- rozhovory MeSH
This study evaluated the effect of low-molecular weight chitosan on Staphylococcus epidermidis, a common colonizer of joint implants and other prosthetic devices. We have also attempted to elucidate its mechanism of action. Chitosan was found to be effective against both the planktonic and biofilm cells (MIC80 35-40 mg/L; MBIC80 40-150 mg/L), in contrast to the antibiotics erythromycin and tetracycline with no antibiofilm activity (MBIC80 not found). In combination, chitosan had an additive effect with antibiotics on suspension growth of S. epidermidis (FICi 0.7-1.0), and the combinatory action caused a complete inhibition of biofilm metabolic activity in some cases. In addition, chitosan caused rapid cellular damage and enhanced antihaemolytic activity of tetracycline in combination towards S. epidermidis biofilm cells. Chitosan efficiently inhibited S. epidermidis growth acting via cell membrane damage, yet the extent of antimicrobial and antibiofilm activities was quite strain-specific. It was proved to be a very efficient antimicrobial agent worth further examination as a potent candidate in pharmaceutical research. Apart from antimicrobial activity, it also acted as antivirulence enhancing agent which is a very promising strategy for alternative infectious diseases treatment.
Globally, the occurrence of biofilm associated infection has become an alarming menace to the medical fraternity because the thick exopolysaccharide layer encasing the biofilms makes the biofilm producing pathogens inherently resistant to antibiotics. Candida albicans, the most common pathogen among Candida spp. is the causative agent for superficial and invasive candidiasis. The morphological phase switching from yeast to hyphal form is one of the virulent traits of C. albicans critical for its pathogenicity. Owing to the emergence of antifungal resistance among this opportunistic fungus, there is a dire need for improvised alternative antifungal agents. In the present study, we have evaluated a biosurfactant from a marine bacterium for its biofilm disruption ability against C. albicans. This biosurfactant had the potential to disrupt biofilms as well as to inhibit the morphological transition from yeast to hyphae. In addition, this biosurfactant showed enhance disruption of mixed species biofilms of C. albicans and Staphylococcus epidermidis when combined with DNase isolated from marine bacteria. From the results obtained, it is evident that the biosurfactant could act as a potential antibiofilm agent against drug resistant C. albicans strains.
- MeSH
- antifungální látky farmakologie MeSH
- Bacteria * enzymologie MeSH
- biofilmy * účinky léků MeSH
- Candida albicans * účinky léků MeSH
- deoxyribonukleasy * metabolismus MeSH
- hyfy MeSH
- kandidóza mikrobiologie MeSH
- lidé MeSH
- Staphylococcus epidermidis účinky léků MeSH
- vodní organismy * enzymologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
The plasma-activated gas is capable of decontaminating surfaces of different materials in remote distances. The effect of plasma-activated water vapor on Staphylococcus epidermidis, methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli biofilm contamination was investigated on the polypropylene nonwoven textile surface. The robust and technically simple multi-hollow surface dielectric barrier discharge was used as a low-temperature atmospheric plasma source to activate the water-based medium. The germicidal efficiency of short and long-time exposure to plasma-activated water vapor was evaluated by standard microbiological cultivation and fluorescence analysis using a fluorescence multiwell plate reader. The test was repeated in different distances of the contaminated polypropylene nonwoven sample from the surface of the plasma source. The detection of reactive species in plasma-activated gas flow and condensed activated vapor, and thermal and electrical properties of the used plasma source, were measured. The bacterial biofilm decontamination efficiency increased with the exposure time and the plasma source power input. The log reduction of viable biofilm units decreased with the increasing distance from the dielectric surface.
- MeSH
- biofilmy účinky léků MeSH
- dekontaminace metody MeSH
- elektřina * MeSH
- Escherichia coli účinky léků MeSH
- methicilin rezistentní Staphylococcus aureus účinky léků MeSH
- mikrobiální viabilita MeSH
- plazmové plyny farmakologie MeSH
- Pseudomonas aeruginosa účinky léků MeSH
- Staphylococcus epidermidis účinky léků MeSH
- Publikační typ
- časopisecké články MeSH
Pterostilbene (PTE) is a naturally occurring compound originally isolated from Pterocarpus spp. It has been widely used in traditional Indian medicine and later discovered to have various beneficial pharmacological effects such as antioxidant properties, hypoglycaemic or antitumor, and antimicrobial activity. This work is focused on demonstrating PTE synergistic effect with erythromycin and tetracycline to reduce their needed effective concentration for suppression of Staphylococcus epidermidis planktonic cells growth and biofilm formation. The secondary aim is to find these combinations effect on the production of its virulence factors. PTE was found to be effective in inhibition of its planktonic cells with MIC80 values 25-37.5 mg l-1. Simultaneously, it decreased the metabolic activity of biofilm cells and was especially effective on a clinical isolate (MBIC80 = 35 mg l-1) in contrast to the conventional antibiotics. In combination, PTE helped the antibiotics to overcome the tolerance of S. epidermidis biofilm cells (5 mg l-1 of each antibiotic with 49 mg l-1 PTE caused more than 85% inhibition of metabolic activity). It permeabilized cytoplasmic membrane of S. epidermidis cells and altered their surface hydrophobicity. Therefore, PTE has a great potential to enhance antibiotics action in the treatment of infections caused by this pathogen.
- MeSH
- nekrotizující enterokolitida terapie MeSH
- novorozenec nedonošený MeSH
- novorozenecká sepse etiologie terapie MeSH
- příčina smrti MeSH
- radiografie MeSH
- rizikové těhotenství MeSH
- Staphylococcus epidermidis patogenita MeSH
- toxoplazmóza diagnóza terapie MeSH
- uživatelé drog MeSH
- vrozená syfilis * diagnóza terapie MeSH
- Check Tag
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
Pterostilbene (PTE), a dimethylated analogue of resveratrol, mostly contained in Vitis vinifera leaves or in other plant sources is well-known for its antioxidant activity. Due to its bioavailability, low hydrophilicity and thus ability to penetrate hydrophobic biological membranes it was found to be an antimicrobial agent. These properties of PTE offer the possibility of its use in the treatment of microbial infections. The emergence of antibiotic resistance of microorganisms is often caused by their ability to form biofilm; new substances with antibiofilm activity are therefore sought. The representatives of opportunistic pathogenic gram-positive and gram-negative bacteria as well as fungi were used for the determination of minimum inhibitory concentrations (MIC50 and MIC80), minimum biofilm inhibitory concentrations (MBIC50 and MBIC80) and minimum biofilm eradication concentrations (MBEC50 and MBEC80) of PTE and commonly used antibiotics erythromycin, polymyxin B or antimycotic amphotericin B. Total biofilm biomass was investigated by crystal violet staining, and the results were confirmed using microscopic techniques. The most significant antibiofilm action was proved for gram-positive cocci, e.g., MBEC50 of PTE for all strains of Staphylococcus epidermidis tested was 25 mg/L. By contrast, the antibiotic ERM did not exhibit antibiofilm activity in most cases. The permeabilization of cell membranes of gram-positive cocci biofilm by MBIC50 and MBEC50 of PTE was confirmed by LIVE/DEAD staining using spinning disc confocal microscopy. PTE significantly influenced the ability of gram-positive cocci to form biofilm and it effectively eradicated pre-formed biofilm in vitro; its potential for the treatment of biofilm-associated infections of Staphylococcus spp. or Enterococcus faecalis is thus apparent.
- MeSH
- antibakteriální látky farmakologie MeSH
- antioxidancia farmakologie MeSH
- biofilmy účinky léků růst a vývoj MeSH
- Enterococcus faecalis účinky léků MeSH
- gramnegativní bakterie účinky léků MeSH
- grampozitivní bakterie účinky léků MeSH
- grampozitivní koky účinky léků MeSH
- listy rostlin chemie MeSH
- mikrobiální testy citlivosti MeSH
- Pseudomonas aeruginosa účinky léků MeSH
- rostlinné extrakty farmakologie MeSH
- Staphylococcus epidermidis účinky léků MeSH
- stilbeny farmakologie MeSH
- Vitis chemie MeSH
- Publikační typ
- časopisecké články MeSH
