The rise of the mosquitoes-transmitted diseases, like dengue, zika and chikungunya in Brazil in the last years has increased concerns on protection against mosquitoes bites. However, the prohibitive prices of the commercially available repellents for the majority of the Brazilian population has provoked a search for cheaper solutions, like the use of the homemade ethanolic extract of Indian clove (Syzygium aromaticum L.) as repellent, which has been reported as quite efficient by the local press. In order to verify this, we performed here the quantification of the main components of this extract through high-performance thin-layer chromatography (HPTLC)-densitometry and evaluated its efficiency as a repellent and its acetylcholinesterase (AChE) inhibition capacity. Our results have proved HPTLC-densitometry as an efficient and appropriate method for this quantification and confirmed the repellency activity, as well as its capacity of AChE inhibition.
- MeSH
- acetylcholinesterasa metabolismus MeSH
- Aedes účinky léků MeSH
- cholinesterasové inhibitory * analýza chemie farmakologie MeSH
- chování zvířat účinky léků MeSH
- chromatografie na tenké vrstvě metody MeSH
- denzitometrie MeSH
- dospělí MeSH
- eugenol analýza chemie farmakologie MeSH
- lidé MeSH
- limita detekce MeSH
- lineární modely MeSH
- mladý dospělý MeSH
- repelenty proti hmyzu * analýza chemie farmakologie MeSH
- reprodukovatelnost výsledků MeSH
- rostlinné extrakty * analýza chemie farmakologie MeSH
- Syzygium chemie MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
With aim to develop effective proof-of-concept approach which can be used in a development of new preparations for the inhalation therapy, we designed a new screening method for simple and rapid simultaneous determination of antibacterial potential of plant volatiles in the liquid and the vapour phase at different concentrations. In addition, EVA (ethylene vinyl acetate) capmat™ as vapour barrier cover was used as reliable modification of thiazolyl blue tetrazolium bromide (MTT) assay for cytotoxicity testing of volatiles on microtiter plates. Antibacterial activity of carvacrol, cinnamaldehyde, eugenol, 8-hydroxyquinoline, thymol and thymoquinone was determined against Haemophilus influenzae, Staphylococcus aureus, and Streptococcus pneumoniae using new broth microdilution volatilization method. The cytotoxicity of these compounds was evaluated using MTT test in lung fibroblast cells MRC-5. The most effective antibacterial agents were 8-hydroxyquinoline and thymoquinone with the lowest minimum inhibitory concentrations (MICs) ranging from 2 to 128μg/mL, but they also possessed the highest toxicity in lung cell lines with half maximal inhibitory concentration (IC50) values 0.86-2.95μg/mL. The lowest cytotoxicity effect was identified for eugenol with IC50 295.71μg/mL, however this compound produced only weak antibacterial potency with MICs 512-1024μg/mL. The results demonstrate validity of our novel broth microdilution volatilization method, which allows cost and labour effective high-throughput antimicrobial screening of volatile agents without need of special apparatus. In our opinion, this assay can also potentially be used for development of various medicinal, agricultural, and food applications that are based on volatile antimicrobials.
- MeSH
- akrolein analogy a deriváty chemie MeSH
- antibakteriální látky chemie MeSH
- benzochinony chemie MeSH
- buněčné linie MeSH
- eugenol chemie MeSH
- fytonutrienty chemie MeSH
- Haemophilus influenzae účinky léků MeSH
- lidé MeSH
- mikrobiální testy citlivosti metody MeSH
- monoterpeny chemie MeSH
- oxychinolin chemie MeSH
- Staphylococcus aureus účinky léků MeSH
- Streptococcus pneumoniae účinky léků MeSH
- těkavé organické sloučeniny chemie MeSH
- tetrazoliové soli MeSH
- thiazoly MeSH
- thymol chemie MeSH
- volatilizace * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Cronobacter sakazakii and C. malonaticus are opportunistic pathogens that cause infections in children and immunocompromised adults. In the present study, the antibacterial activity of 19 plant-derived compounds, 5 essential oils, and an extract of propolis were assessed against C. sakazakii and C. malonaticus. The effects of most of these antimicrobials have not been reported previously. Both strains were susceptible to thymol, carvacrol, thymoquinone, p-cymene, linalool, camphor, citral, eugenol, and trans-cinnamaldehyde as well as cinnamon, lemongrass, oregano, clove, and laurel essential oils; their minimum inhibitory concentrations varied between 0.1 and 2.0 mg/mL. As an alternative treatment method, vapors of the volatiles were tested as an indirect treatment. Vapors of trans-cinnamaldehyde, eugenol, oregano, and cinnamon essential oils inhibited both tested strains, while vapors of linalool were only active against C. sakazakii. To our knowledge, this study is the first time that the inhibitory activity of the vapors of these compounds and essential oils has been reported against Cronobacter spp.
- MeSH
- akrolein analogy a deriváty chemie farmakologie MeSH
- antibakteriální látky chemie farmakologie MeSH
- benzochinony chemie farmakologie MeSH
- Cronobacter sakazakii účinky léků MeSH
- Cronobacter účinky léků MeSH
- Cymbopogon chemie MeSH
- dobromysl (rod) chemie MeSH
- eugenol chemie farmakologie MeSH
- kafr chemie farmakologie MeSH
- mikrobiální testy citlivosti MeSH
- monoterpeny chemie farmakologie MeSH
- oleje prchavé chemie farmakologie MeSH
- oleje rostlin chemie farmakologie MeSH
- rostlinné extrakty chemie farmakologie MeSH
- seskviterpeny chemie farmakologie MeSH
- skořicovník ceylonský chemie MeSH
- Syzygium chemie MeSH
- thymol chemie farmakologie MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Je popsán případ léčby pacienta, kterému byl parenterálně aplikován eugenol (hřebíčkový olej).V klinickém obrazu dominovala methemoglobinémie, cyanóza a nízká saturace hemoglobinu kyslíkem(66–76 %). Pro těžkou tkáňovou hypoxii refrakterní na oxygenoterapii i na transfuze erytrocytů bylaprovedena výměnná erytrocytaferéza. Pro substituci bylo použito 12 transfuzních jednotek deleukotizovanýcherytrocytových koncentrátů s průměrným hematokritem 0,60 (3400 ml), ozářených ionizujícímzářením v dávce 32 Gy, aplikovaných přes transfuzní soupravu. Zůstatková frakce erytrocytůs patologickým hemoglobinem byla ponechána na 20 %. Během výkonu došlo k podstatnému nárůstusaturace hemoglobinu kyslíkem (90 %), nebyla zaznamenána žádná nežádoucí reakce.
The authors describe the case of a pacient who was parenterally administered eugenol (clove oil). Theclinical picture was dominated by methemoglobinemia, cyanosis and low hemoglobin saturation byoxygen (66-76%). In view of a severe tissue hypoxia, which was refractory to oxygenotherapy, erythrocytapheresiswas performed. The substitution included 12 transfusion units of deleukocyted erythrocyteconcentrates with the mean hematocrite 0.60 (3,400 ml), irradiated by ionising radiation at thedose of 32 Gy, administered via a transfusion set. The remaining fraction of erythrocytes with pathologicalhemoglobin was 20%. In the course of the intervention, a substantial increase of hemoglobinsaturation by oxygen developed (90%) and no untoward reaction was noticed.
- MeSH
- cytaferéza metody přístrojové vybavení MeSH
- dospělí MeSH
- eugenol aplikace a dávkování chemie škodlivé účinky MeSH
- krevní obraz MeSH
- lidé MeSH
- methemoglobinemie diagnóza etiologie terapie MeSH
- transfuze erytrocytů MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- přehledy MeSH
- Geografické názvy
- Česká republika MeSH
