In 18 patients with Lyme borreliosis the authors proved the persistence of Borrelia burgdorferi sensu lato by detection of the causal agent by immune electron microscopy or of its DNA by PCR in plasma or cerebrospinal fluid after an interval of 4-68 months. Clinical manifestations common in Lyme borreliosis were present in only half the patients, in the remainder non-specific symptoms were found. In nine subjects with confirmed Borrelia burgdorferi sensu lato in the cerebrospinal fluid the cytological and biochemical finding was normal. Examination of antibodies by the ELISA method was negative in 7 of 18 patients during the first examination and in 12 of 18 during the second examination. In all negative examinations the specific antibodies were assessed by the Western blot or ELISA method after liberation from the immunocomplexes. In the authors' opinion it is advisable to examine repeatedly plasma and other biological material from potentially affected organs by PCR and subjects with persisting or relapsing complaints after the acute form of Lyme borreliosis as well as to examine cerebrospinal fluid in case on non-specific symptoms and concurrent pathic EEG or MR findings.

• MeSH
• Borrelia burgdorferi komplex imunologie   izolace a purifikace   MeSH
• časové faktory MeSH
• DNA bakterií analýza   MeSH
• dospělí MeSH
• imunoelektronová mikroskopie MeSH
• lidé středního věku MeSH
• lidé MeSH
• lymeská nemoc mikrobiologie   MeSH
• mladiství MeSH
• polymerázová řetězová reakce MeSH
• protilátky bakteriální analýza   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA bakterií MeSH
• protilátky bakteriální MeSH

Using a commercial kit for the examination of recombinant immunoblot the authors examined sera of 85 patients with direct evidence of Borrelia burgdorferi sensu lato in serum or cerebrospinal fluid or patients with typical dermal form of borreliosis. The results were compared with results of assessment of specific antibodies by the ELISA test. The authors investigated the importance of antibody formation against different antigens in different clinical forms of the disease. The specificity of the investigated kit was higher than that of the ELISA test, the sensitivity was lower. Examination of the immunoblot was negative in 8.8% patients with direct evidence of the causal agent of Lyme borreliosis. A suitable configuration of the recombinant immunoblot for use in Europe where three genotypes of Borrelia burgdorferi sensu lato are found requires further investigation. Another problem are criteria for evaluation of positivity of the blot in Europe.

• MeSH
• Borrelia burgdorferi komplex imunologie   MeSH
• ELISA MeSH
• imunoblotting * MeSH
• lidé MeSH
• lymeská nemoc diagnóza   MeSH
• protilátky bakteriální analýza   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• protilátky bakteriální MeSH