Clavibacter sp. strain CF11, which was isolated from soil at a tomato-planting greenhouse in Inner Mongolia, North China, has a high capability for producing cold-active cellulase at low temperatures. Here, we report the draft genome sequence of strain CF11, which comprises 2,437 protein-coding sequences and 49 RNA-coding sequences.

• Publikační typ
• časopisecké články MeSH

Chlorophyll (Chl) is an essential component of the photosynthetic apparatus. Embedded into Chl-binding proteins, Chl molecules play a central role in light harvesting and charge separation within the photosystems. It is critical for the photosynthetic cell to not only ensure the synthesis of a sufficient amount of new Chl-binding proteins but also avoids any misbalance between apoprotein synthesis and the formation of potentially phototoxic Chl molecules. According to the available data, Chl-binding proteins are translated on membrane bound ribosomes and their integration into the membrane is provided by the SecYEG/Alb3 translocon machinery. It appears that the insertion of Chl molecules into growing polypeptide is a prerequisite for the correct folding and finishing of Chl-binding protein synthesis. Although the Chl biosynthetic pathway is fairly well-described on the level of enzymatic steps, a link between Chl biosynthesis and the synthesis of apoproteins remains elusive. In this review, I summarize the current knowledge about this issue putting emphasis on protein-protein interactions. I present a model of the Chl biosynthetic pathway organized into a multi-enzymatic complex and physically attached to the SecYEG/Alb3 translocon. Localization of this hypothetical large biosynthetic centre in the cyanobacterial cell is also discussed as well as regulatory mechanisms coordinating the rate of Chl and apoprotein synthesis.

• MeSH
• bakteriální proteiny metabolismus   MeSH
• buněčná membrána metabolismus   MeSH
• chlorofyl metabolismus   MeSH
• fotosyntéza MeSH
• proteiny vázající chlorofyl biosyntéza   MeSH
• sinice cytologie   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• bakteriální proteiny MeSH
• chlorofyl MeSH
• proteiny vázající chlorofyl MeSH

Aerobic anoxygenic phototrophs (AAPs) are bacteriochlorophyll a-containing microorganisms that use organic substrates for growth but can supplement their energy requirements with light. They have been reported from various marine and limnic environments; however, their ecology remains largely unknown. Here infrared epifluorescence microscopy was used to monitor temporal changes in AAPs in the alpine lake Gossenköllesee, located in the Tyrolean Alps, Austria. AAP abundance was low (10(3) cells ml(-1)) until mid-July and reached a maximum of ∼1.3 × 10(5) cells ml(-1) (29% of all prokaryotes) in mid-September. We compared the studied lake with other mountain lakes located across an altitudinal gradient (913 to 2,799 m above sea level). The concentration of dissolved organic carbon and water transparency seem to be the main factors influencing AAP abundance during the seasonal cycle as well as across the altitudinal gradient. While the AAP populations inhabiting the alpine lakes were composed of intensely pigmented large rods (5 to 12 μm), the lakes below the tree line were inhabited by a variety of smaller morphotypes. Analysis of pufM diversity revealed that AAPs in Gossenköllesee were almost exclusively Sphingomonadales species, which indicates that AAP communities inhabiting alpine lakes are relatively homogeneous compared to those in low-altitude lakes.

• MeSH
• Alphaproteobacteria genetika   izolace a purifikace   MeSH
• anaerobióza MeSH
• biodiverzita * MeSH
• chemické jevy MeSH
• DNA bakterií chemie   genetika   MeSH
• fototrofní procesy MeSH
• fylogeneze MeSH
• jezera mikrobiologie   MeSH
• molekulární sekvence - údaje MeSH
• organické látky analýza   MeSH
• roční období MeSH
• sekvenční analýza DNA MeSH
• shluková analýza MeSH
• voda chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Rakousko MeSH
• Názvy látek
• DNA bakterií MeSH
• organické látky MeSH
• voda MeSH

Betaproteobacterial genus Limnohabitans represents an important part of freshwater bacterioplankton. Here, we report genome sequences of two Limnohabitans isolates, Rim28 and Rim47. They contain a complete photosynthesis gene cluster, RuBisCO, CO dehydrogenase, ammonia monooxygenase, and sulfur-oxidizing genes, which indicates a great metabolic versatility of the Limnohabitans species.

• MeSH
• amoniak metabolismus   MeSH
• Betaproteobacteria genetika   MeSH
• Comamonadaceae genetika   MeSH
• fotosyntéza * MeSH
• genom bakteriální * MeSH
• molekulární sekvence - údaje MeSH
• oxidace-redukce MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• amoniak MeSH

Aerobic anoxygenic phototrophs (AAPs) are prokaryotic microorganisms capable of harvesting light using bacteriochlorophyll-based reaction centres. Marine AAP communities are generally dominated by species belonging to the Roseobacter clade. For this reason, we used marine Roseobacter-related strain COL2P as a model organism to characterize its photosynthetic apparatus, level of pigmentation and expression of photosynthetic complexes. This strain contained functional photosynthetic reaction centres with bacteriochlorophyll a and spheroidenone as the main light-harvesting pigments, but the expression of the photosynthetic apparatus was significantly reduced when compared to truly photoautotrophic species. Moreover, the absence of peripheral light-harvesting complexes largely reduced its light-harvesting capacity. The size of the photosynthetic unit was limited to 35.4 +/- 1.0 BChl a molecules supplemented by the same number of spheroidenone molecules. The contribution of oxidative phosphorylation and photophosphorylation was analysed by respiration and fluorometric measurements. Our results indicate that even with a such reduced photosynthetic apparatus, photophosphorylation provides up to three times higher electron fluxes than aerobic respiration. These results suggest that light-derived energy can provide a substantial fraction of COL2P metabolic needs.

• MeSH
• aerobióza genetika   MeSH
• anaerobióza genetika   MeSH
• bakteriální geny MeSH
• bakteriální proteiny genetika   MeSH
• bakteriální RNA analýza   MeSH
• bakteriochlorofyl A genetika   MeSH
• bakteriochlorofyly genetika   MeSH
• fotofosforylace MeSH
• fotosyntetická reakční centra (proteinové komplexy) genetika   MeSH
• fotosyntéza * genetika   MeSH
• fylogeneze MeSH
• karotenoidy metabolismus   MeSH
• konjugace genetická MeSH
• messenger RNA analýza   MeSH
• mořská voda MeSH
• regulace genové exprese u bakterií MeSH
• Roseobacter genetika   metabolismus   MeSH
• sekvence aminokyselin MeSH
• spotřeba kyslíku genetika   MeSH
• světlo MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• bakteriální proteiny MeSH
• bakteriální RNA MeSH
• bakteriochlorofyl A MeSH
• bakteriochlorofyly MeSH
• fotosyntetická reakční centra (proteinové komplexy) MeSH
• karotenoidy MeSH
• messenger RNA MeSH
• spheroidenone MeSH Prohlížeč