Tick cell lines are an easy-to-handle system for the study of viral and bacterial infections and other aspects of tick cellular processes. Tick cell cultures are often continuously cultivated, as freezing can affect their viability. However, the long-term cultivation of tick cells can influence their genome stability. In the present study, we investigated karyotype and genome size of tick cell lines. Though 16S rDNA sequencing showed the similarity between Ixodes spp. cell lines at different passages, their karyotypes differed from 2n = 28 chromosomes for parental Ixodes spp. ticks, and both increase and decrease in chromosome numbers were observed. For example, the highly passaged Ixodes scapularis cell line ISE18 and Ixodes ricinus cell lines IRE/CTVM19 and IRE/CTVM20 had modal chromosome numbers 48, 23 and 48, respectively. Also, the Ornithodoros moubata cell line OME/CTVM22 had the modal chromosome number 33 instead of 2n = 20 chromosomes for Ornithodoros spp. ticks. All studied tick cell lines had a larger genome size in comparison to the genomes of the parental ticks. Thus, highly passaged tick cell lines can be used for research purposes, but possible differences in encoded genetic information and downstream cellular processes, between different cell populations, should be taken into account.

• MeSH
• buněčné kultury metody   MeSH
• buněčné linie MeSH
• Ixodidae genetika   MeSH
• karyotyp MeSH
• klíšťata genetika   růst a vývoj   MeSH
• Ornithodoros genetika   MeSH
• RNA ribozomální 16S genetika   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• RNA ribozomální 16S MeSH

DNA methylation at the fifth position of cytosine (5mC) and at the sixth position of adenine (6 mA) plays an important role in the regulation of the gene expression and, in eukaryotes, is essential for normal development. For Ixodes ricinus, the most common European arthropod vector of human and animal pathogens, the DNA methylation profile and the role of DNA methylation in tick development are still under discussion. Our goal was to analyze the status of I. ricinus DNA methylation at different life stages and identify enzymes that produce this type of DNA modification. We found that 5mC and 6mA are present in I. ricinus genomic DNA at all life stages. In the transcriptome of I. ricinus, we identified the sequences of the putative IrDNMT1, IrDNMT3, and IrDAMT enzymes, and bioinformatic analysis and three-dimensional modeling predicted their DNA methylation activity. This confirms that I. ricinus possesses a complete DNA methylation toolkit. Our results suggest that DNA methylation is important for the physiology and transstadial development of ticks.

• Klíčová slova
• 5-Methylcytosine, DNA methylation, Epigenetics, Ixodes tick, Methyltransferase, N6-Methyladenine,
• MeSH
• epigeneze genetická * MeSH
• genetická transkripce MeSH
• klíště enzymologie   genetika   růst a vývoj   MeSH
• larva genetika   růst a vývoj   MeSH
• methyltransferasy chemie   genetika   MeSH
• molekulární konformace MeSH
• nymfa genetika   růst a vývoj   MeSH
• ovum růst a vývoj   MeSH
• proteiny členovců chemie   genetika   MeSH
• regulace genové exprese * MeSH
• transkriptom * MeSH
• věkové faktory MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• methyltransferasy MeSH
• proteiny členovců MeSH

The inducible repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) eliminates O6-methylguanine adducts in DNA and protects the cells from damaging effects of alkylating agents. We have found that anti-MGMT antibodies recognize both the MGMT protein with a mol. weight ~ 24 kDa and a protein with a mol. weight ~ 48 kDa, which was named MARP (anti-methyltransferase antibody recognizable protein). A number of growth factors and cytokines were shown to regulate the expression of MGMT and MARP proteins. The ranges of concentrations of several growth factors and cytokines that caused increasing or decreasing protein amounts in human cell cultures were determined. The results of special biological experiments have allowed us to assume a possible role of MARP in the repair of alkyl adducts in human cells.

• Klíčová slova
• Antibody, Cytokine, DNA damage, Growth factor, Human cells in vitro, Repair enzyme MGMT,
• MeSH
• buněčné kultury MeSH
• buněčné linie MeSH
• buňky Hep G2 MeSH
• cytokiny farmakologie   MeSH
• DNA modifikační methylasy genetika   MeSH
• enzymy opravy DNA genetika   MeSH
• lidé MeSH
• mezibuněčné signální peptidy a proteiny farmakologie   MeSH
• nádorové supresorové proteiny genetika   MeSH
• regulace genové exprese účinky léků   MeSH
• techniky in vitro MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• cytokiny MeSH
• DNA modifikační methylasy MeSH
• enzymy opravy DNA MeSH
• mezibuněčné signální peptidy a proteiny MeSH
• MGMT protein, human MeSH Prohlížeč
• nádorové supresorové proteiny MeSH