Antibacterial biodegradable PLA-based nanofibers loaded with phenolic monoterpenes - thymol, eugenol, carvacrol, and cinnamaldehyde, were prepared by electrospinning. The effect of bioactive molecule on the surface, thermal, morphological, and biological properties has been investigated about the potential pharmaceutical and food processing applications. Fiber diameters ranged from 320 nm for PLA fibrous mat up to 480 nm for PLA membrane with 6 % thymol. All the prepared active nanofibers exhibited hydrophobic surfaces with a slightly decreasing contact angle after the incorporation of phenols. Antimicrobial testing proved a strong efficiency against Escherichia coli and Staphylococcus aureus, depending on the specific type and content of the bioactive compound. A significant biofilm formation reduction of bioactive PLA nanofibers was revealed against tested microorganisms. Modification of PLA fibers with active molecules did not significantly affect the biodegradation kinetics in comparison to PLA samples with their absence. This study demonstrates the high potential of newly developed PLA-based/phenol nanofibrous membranes for use as antibacterial and antifouling systems applicable in wound dressings and food packaging.
- Klíčová slova
- Antibacterial, Antifouling, Biodegradable, Biomedical applications, Electrospinning, Polylactic acid, Sustainable,
- Publikační typ
- časopisecké články MeSH
Prostate cancer (PCa) is the second most common cancer. In this paper, the isolation and properties of exosomes as potential novel liquid biopsy markers for early PCa liquid biopsy diagnosis are investigated using two prostate human cell lines, i.e., benign (control) cell line RWPE1 and carcinoma cell line 22Rv1. Exosomes produced by both cell lines are characterised by various methods including nanoparticle-tracking analysis, dynamic light scattering, scanning electron microscopy and atomic force microscopy. In addition, surface plasmon resonance (SPR) is used to study three different receptors on the exosomal surface (CD63, CD81 and prostate-specific membrane antigen-PMSA), implementing monoclonal antibodies and identifying the type of glycans present on the surface of exosomes using lectins (glycan-recognising proteins). Electrochemical analysis is used to understand the interfacial properties of exosomes. The results indicate that cancerous exosomes are smaller, are produced at higher concentrations, and exhibit more nega tive zeta potential than the control exosomes. The SPR experiments confirm that negatively charged α-2,3- and α-2,6-sialic acid-containing glycans are found in greater abundance on carcinoma exosomes, whereas bisecting and branched glycans are more abundant in the control exosomes. The SPR results also show that a sandwich antibody/exosomes/lectins configuration could be constructed for effective glycoprofiling of exosomes as a novel liquid biopsy marker.
- Klíčová slova
- exosomes, microscopy techniques, nanoparticle tracking analysis, prostate cancer, self-assembled monolayer, surface plasmon resonance,
- MeSH
- exozómy * chemie MeSH
- karcinom * metabolismus patologie MeSH
- lektiny analýza metabolismus MeSH
- lidé MeSH
- polysacharidy analýza metabolismus MeSH
- tekutá biopsie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- lektiny MeSH
- polysacharidy MeSH
In this study, fibrous membranes from recycled-poly(ethylene terephthalate)/silk fibroin (r-PSF) were prepared by electrospinning for filtration applications. The effect of silk fibroin on morphology, fibers diameters, pores size, wettability, chemical structure, thermo-mechanical properties, filtration efficiency, filtration performance, and comfort properties such as air and water vapor permeability was investigated. The filtration efficiency (FE) and quality factor (Qf), which represents filtration performance, were calculated from penetration through the membranes using aerosol particles ranging from 120 nm to 2.46 μm. The fiber diameter influenced both FE and Qf. However, the basis weight of the membranes has an effect, especially on the FE. The prepared membranes were classified according to EN149, and the most effective was assigned to the class FFP1 and according to EN1822 to the class H13. The impact of silk fibroin on the air permeability was assessed. Furthermore, the antibacterial activity against bacteria S. aureus and E. coli and biocompatibility were evaluated. It is discussed that antibacterial activity depends not only on the type of used materials but also on fibrous membranes' surface wettability. In vitro biocompatibility of the selected samples was studied, and it was proven to be of the non-cytotoxic effect of the keratinocytes (HaCaT) after 48 h of incubation.
- Klíčová slova
- air filtration, antibacterial activity, comfort properties, electrospun membrane, poly(ethylene terephthalate), silk fibroin,
- Publikační typ
- časopisecké články MeSH
The first-line chemotherapy of colorectal cancer (CRC), besides surgery, comprises administration of 5-Fluorouracil (5FU). Apart from cytotoxic effect on cancer cells, 5FU may also cause adverse side effects. Ganoderma Lucidum (GLC) is a mushroom used in Traditional Eastern Medicine. We propose that natural compounds, particularly GLC extracts, may sensitize cancer cells to conventional chemotherapeutics. This combination therapy could lead to more selective cancer cell death and may improve the response to the therapy and diminish the adverse effects of anticancer drugs. Here we demonstrate that GLC induced oxidative DNA damage selectively in colorectal cancer cell lines, whereas it protected non-malignant cells from the accumulation of reactive oxygen species. Accumulation of DNA damage caused sensitization of cancer cells to 5FU resulting in improved anticancer effect of 5FU. The results obtained in colorectal cell lines were confirmed in in vivo study: GLC co-treatment with 5FU increased the survival of treated mice and reduced the tumor volume in comparison with group treated with 5FU alone. Combination of conventional chemotherapeutics and natural compounds is a promising approach, which may reduce the effective curative dose of anticancer drugs, suppress their adverse effects and ultimately lead to better quality of life of CRC patients.
- Klíčová slova
- 5-Fluorouracil, Colorectal cancer, Combination therapy, DNA damage, Ganoderma Lucidum,
- MeSH
- adenokarcinom farmakoterapie patologie MeSH
- buněčné dělení účinky léků MeSH
- DNA nádorová účinky léků MeSH
- fluoruracil farmakologie terapeutické užití MeSH
- invazivní růst nádoru MeSH
- kolorektální nádory farmakoterapie patologie MeSH
- kometový test MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- nádorové buněčné linie MeSH
- oxidační stres MeSH
- poškození DNA * MeSH
- protinádorové antimetabolity farmakologie terapeutické užití MeSH
- reaktivní formy kyslíku metabolismus MeSH
- reishi chemie MeSH
- rostlinné extrakty izolace a purifikace farmakologie MeSH
- screeningové testy protinádorových léčiv MeSH
- synergismus léků MeSH
- testy nádorových kmenových buněk MeSH
- tumor burden účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA nádorová MeSH
- fluoruracil MeSH
- protinádorové antimetabolity MeSH
- reaktivní formy kyslíku MeSH
- rostlinné extrakty MeSH
