The study is based on a longitudinal cephalometric follow-up of X-ray films of patients with a complete unilateral cleft lip and palate during puberty. Carthesian x, y coordinates of the total of 22 landmarks on the lateral films of 25 patients with the same diagnosis and therapy were established, which served as outcome data for shape analysis. The changes in facial shape experienced by patients between their 10th and 15th year were evaluated by the thin-plate splines (TPS) method. We used the TPS method to find a function which transforms the shape of the face at the age of 10 as well as the square TPS grid into the facial shape at the age of 15 years with the deformed TPS grid. With the help of Geometric PCA for Bookstein's coordinates we found individuals with different shapes who develop abnormally during puberty and whose development is not well predictable. During puberty the face becomes relatively elongated. The most pronounced deformation is caused by the shift of the first permanent molar anteriorly, or a relative shortening of the frontal part of the dental arch. In the anterior direction the position of landmarks changed only in the area of nose; the alveolar process shows retrusion. The position of the mandible in relation to the maxilla change mainly in the inferior direction; the mandibular angle shifts posteriorly. The shape of skeletal profile was compared with a group of patients with the same diagnosis who underwent different therapy.
- MeSH
- dítě MeSH
- kefalometrie metody MeSH
- kraniofaciální abnormality diagnostické zobrazování MeSH
- lebka růst a vývoj MeSH
- lidé MeSH
- lineární modely MeSH
- mladiství MeSH
- následné studie MeSH
- nosní přepážka chirurgie MeSH
- radiografie MeSH
- rozštěp patra chirurgie MeSH
- rozštěp rtu chirurgie MeSH
- studie případů a kontrol MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
BACKGROUND: Celiac sprue is considered to be the second most common chronic disease in childhood after allergic diseases. At present, the prevalence of this disease is stated as high as approximately 1% in inhabitants of the North America and Western Europe. Aetiology of celiac sprue is multifactorial as it is in other chronic diseases. Pathogenetically, it is an autoimmune disease whose main autoantigene is the tissue transglutaminase. It affects those individuals carrying HLA-DO2 or HLA-DO8 gene and those who were exposed to wheat gliadine or similar amino acids (prolamines) in rye and barley. The purpose of the present study was to find out whether clinical manifestation of celiac sprue changed in our group of patients in course of 23 years. METHODS AND RESULTS: In forty-eight children celiac sprue was diagnosed according to histopathological and histochemical findings in the small intestine mucosa. In the children examined within 5 years in 1982-1987, main clinical symptom of celiac sprue was diarrhoea. In the control group of children examined within 7 months in 2004-2005, intestinal and extraintestinal symptoms in celiac sprue were equally distributed; the so-called sleeping forms occurred too. CONCLUSIONS: It is discussed what are the trigger mechanisms and the possible danger of celiac sprue manifestations and why celiac sprue is diagnosed in the older children at present time compared with the past years.
- MeSH
- celiakie komplikace diagnóza genetika patologie MeSH
- dítě MeSH
- jehlová biopsie MeSH
- lidé MeSH
- předškolní dítě MeSH
- průjem etiologie MeSH
- střevní sliznice patologie MeSH
- věkové faktory MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
BACKGROUND: There exist only inconsistent results of studies on the relationship between Helicobacter pylori (H. pylori) infection and functional dyspepsia and/or recurrent abdominal pain in children and adolescents. The answer could bring a comparison of the epidemiological features of H. pylori infection between children and adolescents with dyspepsia and/or recurrent abdominal pain (symptomatic) and without these symptoms (asymptomatic), living in the Czech republic. METHODS AND RESULTS: Study includes children and adolescents (2-18 years) with upper gastrointestinal symptoms visiting paediatric gastroenterology department between 1994 and 1999. Age, sex, socioeconomic level matched control (asymptomatic) group consisted of children and adolescents visiting the general paediatric service with symptoms not related to the upper gastrointestinal tract. Demographic and socioeconomic factors, including parent(s) educational level, place of residence, living conditions, type of drinking water and pets in their household were evaluated. Serum of the children and adolescents was tested for H. pylori IgG by enzyme linked immunosorbent assay. In addition, the symptomatic children and adolescents underwent endoscopic examination and biopsies from gastric antrum and corpus were taken for the quick urease test, histology/histoscopy, and H. pylori cultivation. H. pylori infection in this group was stated when at least two tests were positive. Altogether 829 children and adolescents were examined, 624 cases were symptomatic and 205 represented controls (asymptomatic). The prevalence of infection was 33% among symptomatic children vs. 7.5% among controls (OR = 6.2, p.001) and was similar among boys and girls (32% vs. 34.5%, respectively). H. pylori prevalence increased with age among symptomatic children (10% for children below 6 years and 37% between 11 to 16 years) (p.001). In contrary, prevalence tended to fall with age among asymptomatic children (11% in children below 6 years vs. 6% in children over 10 years (OR = 2.0, 95% CI = 0.7-6.2). In both groups, an inverse correlation between H. pylori occurrence and mother's educational level was observed. The H. pylori incidence was 10% among asymptomatic children who drank water from municipal water supply or from a well vs. 3% among those who drank bottled water (OR = 4, 95% CI = 1.1 to 18, p.05). CONCLUSIONS: H. pylori infection was more prevalent among symptomatic children and adolescents vs. asymptomatic children and adolescents within the same population. H. pylori incidence increased with age among symptomatic children and adolescents and tended to fall among controls, showing deep differences between the two groups. With exception of the bottled water drinking, presence of any other factor studied represented significant risk for acquiring the H. pylori infection.
- MeSH
- dítě MeSH
- Helicobacter pylori * MeSH
- incidence MeSH
- infekce vyvolané Helicobacter pylori diagnóza epidemiologie MeSH
- lidé MeSH
- mladiství MeSH
- předškolní dítě MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
OBJECTIVE: To determine the structure (disease) modifying effect of a glycosaminoglycan polypeptide association complex (GP-C; Rumalon) in patients with knee and hip osteoarthritis (OA). METHODS: Double-blind, randomized, placebo-controlled five-year study. Primary assessment criterion was change in radiographic joint space width between baseline and follow-up at 5 years. Secondary outcome criteria included Lequesne algofunctional index (LAI), pain on passive motion and consumption of non-steroidal antiinflammatory drugs (NSAIDs). The patients received 10 courses of injections of placebo or GP-C 2 ml intramuscularly in 5 years (two courses each year). Each course included 15 injections administered twice weekly. RESULTS: There were 277 patients with knee OA and 117 patients with hip OA. Control and GP-C treated groups were comparable as to sex, age, duration of disease, body weight, X-ray stage and value of LAI at the baseline. Knee joint space at 5 years decreased 0.37+/-0.08 (mean+/-standard deviation) mm for GP-C and 0.42+/-0.08 mm for placebo groups (P=0.68). Hip joint space at 5 years decreased 0.21+/-0.08 mm for GP-C and 0.22+/-0.08 mm for placebo groups (P=0.53). In a subset of patients with hip OA, Kellgren-Lawrence> or =2 and JSW> or =1 mm, there was a trend in favor of GPC for lower joint space narrowing in 5 years (P=0.11). In addition, there were no statistical differences between the treatment groups in LAI, pain on passive motion and consumption of NSAIDs. Side-effects after GP-C (14.5%) were rare, mild and not more frequent than in the placebo group (15%). CONCLUSION: We were not able to demonstrate a structure modifying effect of GP-C in OA of the hip or knee. Radiographic progression of OA in both knee and hip OA was lower than expected in both study groups.
- MeSH
- antirevmatika škodlivé účinky terapeutické užití MeSH
- artróza kolenních kloubů farmakoterapie patologie MeSH
- artróza kyčelních kloubů farmakoterapie patologie MeSH
- dospělí MeSH
- dvojitá slepá metoda MeSH
- glykosaminoglykany škodlivé účinky terapeutické užití MeSH
- lidé středního věku MeSH
- lidé MeSH
- následné studie MeSH
- progrese nemoci MeSH
- prospektivní studie MeSH
- senioři MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- klinické zkoušky MeSH
- multicentrická studie MeSH
- práce podpořená grantem MeSH
- randomizované kontrolované studie MeSH
- Názvy látek
- A73025 MeSH Prohlížeč
- antirevmatika MeSH
- glykosaminoglykany MeSH
Silver stained proteins (SSPs) characteristic for interphasic nucleolus organizer regions (NORs) associated with fibrillar centers (FCs) and adjacent nucleolar regions of ring shaped nucleoli in leukemic lymphocytes exhibit a different sensitivity to the mild acid extraction including that with HCl. Such extractions permit a preferential visualization of fibrillar centers adjacent regions (FCARs) which are believed to represent sites of the ribosomal RNA (rRNA) transcription. The resistance of SSPs in FCARs to the extraction with HCl seems to be due to their binding to other components present in these regions. The extractibility of SSPs with HCl was influenced by the fixatives used. The largest resistance of SSPs to the extraction with HCl was noted after fixation with glutaraldehyde. In contrast, the largest extractibility of these proteins was observed after fixation with unbuffered formaldehyde.
- MeSH
- B-lymfocyty chemie ultrastruktura MeSH
- barvení stříbrem metody MeSH
- buněčné jadérko chemie ultrastruktura MeSH
- chronická lymfatická leukemie patologie MeSH
- fixace tkání MeSH
- HeLa buňky chemie ultrastruktura MeSH
- jaderné proteiny analýza MeSH
- kyselina chlorovodíková chemie MeSH
- lidé MeSH
- organizátor jadérka chemie ultrastruktura MeSH
- stříbro chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- jaderné proteiny MeSH
- kyselina chlorovodíková MeSH
- stříbro MeSH
UNLABELLED: The ultrastructure of sperm cells in cumulus oophorus of the human oocyte 20 hrs after in vitro fertilization (IVF) was studied. MATERIALS AND METHODS: Mechanically removed cumular cells were processed for electron microscopy and the spermatozoa deposited in the intracellular spaces as well as those penetrating into follicular cells were evaluated. The samples were divided into three groups. Group I contained cumuli from unfertilized oocytes, group II involved those from fertilized oocytes, but not pregnancy after embryo transfer. Group III was formed by cumuli from fertilized oocytes of patients, where pregnancy had been achieved. RESULTS: In group I numerous anomalies of spermatozoal ultrastructure were found, such as head malformations, incompleted chromatin condensation, large cytoplasmatic droplets, etc. In groups II and III, about 70% normal sperm cells were found. CONCLUSIONS: These findings provide evidence of the important role of the morphological quality of spermatozoa in the failure of in vitro fertilization, also in cases where anomalies could not be detected by the usual methods of sperm examination.
Gastric mucosa ultrastructure was studied in 32 children and adolescents with chronic superficial active and inactive gastritis. Helicobacter pylori was found most frequently free in mucous layer without direct contact with the mucosa. But in 7 children (21.9%), it occurred quite close to superficial epithelial cells and influenced their outlook. In two cases, it was registered in intracytoplasmic channels of intact parietal cells. Another two children with massive helicobacterial infection showed superficial epithelial cells with big mucus vacuoles and sequestration of apical cytoplasm.
- MeSH
- chronická nemoc MeSH
- dítě MeSH
- gastritida mikrobiologie patologie MeSH
- Helicobacter pylori * MeSH
- infekce vyvolané Helicobacter pylori patologie MeSH
- lidé MeSH
- mladiství MeSH
- žaludeční sliznice mikrobiologie ultrastruktura MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND: The importance of Helicobacter pylori for the development of gastritis and gastro-duodenal ulceration is generally accepted. The objective of the presented work was to find out whether its transmission is involved in families of children examined on account of upper dyspeptic syndrome. Aggregation and transmission of Helicobacter pylori infection were not described so far in the Czech Republic. METHODS AND RESULTS: The authors examined IgG antibodies (ELISA) against Helicobacter pylori in 411 subjects incl. 102 children with non-ulcerative or ulcerative dyspepsia and in 115 children of similar age but without gastrointestinal complaints and in 194 family members of children with dyspepsia. The specificity of the ELISA test is 97.1% and the sensitivity 92.1% (discrimination analysis). Children with dyspepsia had more frequently a positive finding of IgG antibodies against H. pylori (p = 0.0001), as compared with the control group of children of the same age but without dyspepsia. A statistically significant source of infection with H. pylori for dyspeptic children was the mother (p = 0.0006) and both parents (p = 0.0069), who were also a source of infection for other family members (p = 0.0003 and 0.0084). The most frequent positive IgG finding was in fathers (76.7%), but the father was not a statistically significant source of infection for the examined children (p = 0.6120) nor for other members of the family (p = 0.2330). IgG positive mothers had 85.2% positive husbands, IgG negative mothers had 68.4% positive husbands (p = 0.1601). The frequency of IgG antibodies in the mother and father (in couples) was thus statistically independent. However, there was a significant correlation (p = 0.01) of (P/N) IgG antibodies in couples--i.e. father and mother of the examined children. Siblings of IgG positive children had a significantly higher mean value of antibodies (p = 0.05) and were older. CONCLUSIONS: The presented facts provide evidence that in the investigated group the Helicobacter pylori infection is more frequent in children with the upper dyspeptic syndrome than in children of similar age without gastrointestinal complaints. In families of children with dyspepsia the infection aggregates and is transmitted. The mother and both parents are involved in the transmission to children. Nevertheless it is probable that there is also mutual transmission in children and from adults to children outside the family and in another environment.
- MeSH
- dítě MeSH
- dospělí MeSH
- dyspepsie mikrobiologie MeSH
- Helicobacter pylori * imunologie MeSH
- imunoglobulin G analýza MeSH
- infekce vyvolané Helicobacter pylori přenos MeSH
- lidé MeSH
- mladiství MeSH
- peptický vřed mikrobiologie MeSH
- protilátky bakteriální analýza MeSH
- zdraví rodiny * MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- imunoglobulin G MeSH
- protilátky bakteriální MeSH
The distribution of silver stained proteins (SSPs) was investigated in ring shaped nucleoli of lymphocytes of the peripheral blood of patients suffering from chronic lymphocytic leukemia (B type). As expected, SSPs were present in fibrillar centers and adjacent nucleolar regions which appeared as bud and protrusion-like structures. The inhibition of the nucleolar RNA transcription by cultivation of leukemic lymphocytes at low temperature (4 degrees C) resulted in a significant reduction of these structures at fibrillar centers of ring-shaped nucleoli and decreased the number of cells which contained a large number of such structures at fibrillar centers of their ring shaped nucleoli. There was no substantial difference in the visualization and distribution of SSPs after fixation with phosphate-buffered glutaraldehyde or formaldehyde in distilled water and postfixation in ethanol or methanol-glacial acetic acid mixture.
- MeSH
- barvení a značení MeSH
- buněčné jadérko patologie ultrastruktura MeSH
- chronická lymfatická leukemie krev patologie MeSH
- elektronová mikroskopie MeSH
- exprese genu MeSH
- formaldehyd MeSH
- genetická transkripce MeSH
- glutaraldehyd MeSH
- histologické techniky MeSH
- jaderné proteiny analýza MeSH
- kultivované buňky MeSH
- lidé MeSH
- lymfocyty cytologie patologie ultrastruktura MeSH
- nízká teplota MeSH
- referenční hodnoty MeSH
- RNA nádorová biosyntéza MeSH
- stříbro MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- formaldehyd MeSH
- glutaraldehyd MeSH
- jaderné proteiny MeSH
- RNA nádorová MeSH
- stříbro MeSH
We studied the efficacy and tolerability of glycosaminoglycan polysulfuric acid (GAGPS) in 80 patients with osteoarthritis (OA) of the knee. Patients received two series of five intra-articular injections, at 1-week intervals, of 25 mg (0.5 ml) GAGPS into the knee in a double-blind, parallel, randomized, placebo-controlled trial. There was an immediate decrease in pain after the injections of 43% with GAGPS and 33% with placebo (P = 0.047) (Jezek pain index). Pain relief of GAGPS vs placebo was not different at other intervals (10, 14, 22, 26 weeks after start of treatment). At 6 weeks the Lequesne index decreased 20% after GAGPS and 9% after placebo (P = 0.17). At 10 weeks the Lequesne index decreased 24% after GAGPS and 13% after placebo (P = 0.20). The decrease in Lequesne index at 14 weeks was 31% after GAGPS and 15% after placebo (P = 0.06). The other measured parameters tended to be more favorably influenced by GAGPS than placebo. GAGPS was well tolerated, with associated mild adverse reactions in 8% of cases. GAGPS may have a role as a symptomatic slow acting drug for OA. Further study appears appropriate.
- MeSH
- antiflogistika nesteroidní terapeutické užití MeSH
- dvojitá slepá metoda MeSH
- glykosaminoglykany aplikace a dávkování škodlivé účinky terapeutické užití MeSH
- ibuprofen terapeutické užití MeSH
- injekce intraartikulární MeSH
- kolenní kloub * MeSH
- lidé středního věku MeSH
- lidé MeSH
- měření bolesti MeSH
- osteoartróza farmakoterapie patofyziologie MeSH
- paliativní péče MeSH
- senioři MeSH
- spokojenost pacientů MeSH
- stupeň závažnosti nemoci MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- klinické zkoušky MeSH
- randomizované kontrolované studie MeSH
- Názvy látek
- antiflogistika nesteroidní MeSH
- Arteparon MeSH Prohlížeč
- glykosaminoglykany MeSH
- ibuprofen MeSH
