The termination of different subsets in apparently homogeneous cell population of T lymphocytes seems to be extremely important in the investigation of allergic diseases and can be of diagnostical use. It has been proved that the dysfunction of those subsets is very important pathogenetic mechanism of atopy. The existing technique using monoclonal antibodies to cell surface markers is rather expensive and has even some disadvantages. It has been published recently that some strains of bacteria were capable to bind spontaneously to human lymphocytes. Simultaneous use of more bacterial strains permitted to identify two B-cells and four T-cells subsets. The interaction of lectins on the lymphocyte surface and polysaccharide substances of the bacterial cell wall is supposed to be the mechanism of binding. In the present study an attempt was made to find out whether there is a difference in the binding of bacteria to lymphocytes between normal subjects and atopics and whether changes occur in this phenomenon after application of immunotherapy. It has been found that this qualitatively novel way of binding cannot be used for laboratory characterization of atopic subjects. No differences were observed in T-lymphocyte subsets identified by bacterial adherence between allergic and normal subjects. Immunotherapy failed to influence the binding. No correlation has been found with the method using monoclonal antibodies. Statistical evaluation revealed considerable dispersion of results obtained.
- MeSH
- B-lymfocyty klasifikace MeSH
- bakteriální adheze * MeSH
- časná přecitlivělost krev diagnóza terapie MeSH
- desenzibilizace imunologická MeSH
- lidé MeSH
- lymfocyty klasifikace imunologie MeSH
- monoklonální protilátky MeSH
- T-lymfocyty klasifikace MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- monoklonální protilátky MeSH
- MeSH
- anestezie * MeSH
- B-lymfocyty klasifikace imunologie MeSH
- dítě MeSH
- kardiochirurgické výkony * MeSH
- lidé MeSH
- mimotělní oběh * MeSH
- předškolní dítě MeSH
- T-lymfocyty klasifikace imunologie MeSH
- vrozené srdeční vady chirurgie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- předškolní dítě MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
B lymphocytes with receptors specific for a particular hapten have been prepared through an antigen-affinity procedure. Methods have been developed for the clonal stimulation of these cells in vitro, with a single, hapten-specific B cell as the unequivocal target. Many stimulatory combinations involve multivalent antigen and one or more antigen non-specific, non-MHC restricted T lymphocyte-derived growth and differentiation factors. These factors, of which there are at least 4 or 5, are progressively being defined and should soon become available through recombinant DNA technology. Judicious use of factor combinations and selected antigens should soon answer whether "T-independent" and "T-dependent" B cells are truly separate subsets. A contact between multivalent antigen and immature B cell in the absence of these co-stimulatory factors can lead to the receipt and storage of a negative signal by the B cell. The B cell is not killed, but rather rendered anergic. Whether clonal anergy among B cells is an important mechanism in physiologic self-tolerance remains to be determined.
- MeSH
- aktivace lymfocytů MeSH
- antigeny imunologie MeSH
- B-lymfocyty klasifikace cytologie imunologie MeSH
- buněčná diferenciace MeSH
- imunologická tolerance MeSH
- lidé MeSH
- lymfokiny imunologie MeSH
- tvorba protilátek * MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Názvy látek
- antigeny MeSH
- lymfokiny MeSH
- MeSH
- B-lymfocyty klasifikace MeSH
- buněčná adheze MeSH
- lidé MeSH
- separace buněk metody MeSH
- tvorba rozet metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- srovnávací studie MeSH
