Alginate lyases have countless potential for application in industries and medicine particularly as an appealing biocatalyst for the production of biofuels and bioactive oligosaccharides. Solid-state fermentation (SSF) allows improved production of enzymes and consumes less energy compared to submerged fermentation. Seaweeds can serve as the most promising biomass for the production of biochemicals. Alginate present in the seaweed can be used by alginate lyase-producing bacteria to support growth and can secrete alginate lyase. In this perspective, the current study was directed on the bioprocessing of brown seaweeds for the production of alginate lyase using marine bacterial isolate. A novel alginate-degrading marine bacterium Enterobacter tabaci RAU2C which was previously isolated in the laboratory was used for the production of alginate lyase using Sargassum swartzii as a low-cost solid substrate. Process parameters such as inoculum incubation period and moisture content were optimized for alginate lyase production. SSF resulted in 33.56 U/mL of alginate lyase under the static condition maintained with 75% moisture after 4 days. Further, the effect of different buffers, pH, and temperature on alginate lyase activity was also analyzed. An increase in alginate lyase activity was observed with an increase in moisture content from 60 to 75%. Maximum enzyme activity was perceived with phosphate buffer at pH 7 and 37 °C. Further, the residual biomass after SSF could be employed as biofertilizer for plant growth promotion based on the preliminary analysis. To our knowledge, this is the first report stating the usage of seaweed biomass as a substrate for the production of alginate lyase using solid-state fermentation.
- Klíčová slova
- Enterobacter tabaci RAU2C, Alginate lyase, Seaweeds, Solid-state fermentation,
- MeSH
- algináty * metabolismus MeSH
- biomasa MeSH
- Enterobacter * metabolismus enzymologie izolace a purifikace růst a vývoj MeSH
- fermentace * MeSH
- koncentrace vodíkových iontů MeSH
- kyselina glukuronová metabolismus MeSH
- mořské řasy * mikrobiologie MeSH
- Phaeophyceae mikrobiologie MeSH
- polysacharid-lyasy * metabolismus MeSH
- Sargassum * mikrobiologie metabolismus MeSH
- teplota MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- algináty * MeSH
- kyselina glukuronová MeSH
- poly(beta-D-mannuronate) lyase MeSH Prohlížeč
- polysacharid-lyasy * MeSH
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální léková rezistence účinky léků genetika MeSH
- bakteriální proteiny genetika metabolismus MeSH
- beta-laktamasy genetika metabolismus MeSH
- DNA bakterií MeSH
- Enterobacter účinky léků enzymologie genetika MeSH
- Enterobacteriaceae účinky léků enzymologie genetika MeSH
- karbapenemy farmakologie MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- dopisy MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- antibakteriální látky MeSH
- bakteriální proteiny MeSH
- beta-laktamasy MeSH
- carbapenemase MeSH Prohlížeč
- DNA bakterií MeSH
- karbapenemy MeSH
Extended-spectrum beta-lactam antibiotics have found great medical importance, but their wide use in clinical practice leads to increasing resistance to them. The more frequent occurrence of infections caused by Bush group 1 beta-lactamase producing organisms, including species of the genus Enterobacter, is a serious problem in this field. Resistance to beta-lactams in this important nosocomial pathogens can be due to 1) reduction in outer membrane permeability to antibiotics caused by alterations in outer membrane lipopolysacharides or proteins (porins); 2) production of beta-lactamases, which inactivate beta-lactams and can also lead to resistance by non-hydrolytic mechanism called trapping. Production of plasmid-mediated extended-spectrum beta-lactamases, but especially chromosomally-mediated inducible cephalosporinase AmpC, which can be synthesized constitutively in large amounts as consequence of spontaneous chromosomal mutations, are of great clinical importance. Fourth-generation cephalosporins and carbapenems are the most effective in the treatment of infections caused by species belonging to the genus Enterobacter, but combination of high level beta-lactamase production and decreased outer membrane permeability, which is not rare in Enterobacter spp., leads to resistance even to these drugs.
Enterobacter sakazakii is the new species name introduced in 1977 for yellow-pigmented strains originally designated as yellow Enterobacter cloacae. All of the six E.sakazakii strains isolated from powdered milk specimens were found to produce Tween 80 esterase after 7 days of incubation at 25 degrees C and 37 degrees C. From E.cloacae it is distinguishable by reactions in four, or even three, biochemical tests, i.e. by production of yellow pigment, positive production of Tween 80 esterase and by non-fermentation of sorbite and mucate; from the Serratia species it can be differentiated by the negative test for lecithinase production.
- MeSH
- Enterobacter enzymologie izolace a purifikace MeSH
- Enterobacteriaceae izolace a purifikace MeSH
- karboxylesterhydrolasy analýza MeSH
- mléko mikrobiologie MeSH
- potrava pro kojence analýza MeSH
- potrava speciální analýza MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- karboxylesterhydrolasy MeSH
- tween 80 esterase MeSH Prohlížeč
- MeSH
- chymotrypsin metabolismus MeSH
- Enterobacter enzymologie MeSH
- Enterococcus faecalis enzymologie MeSH
- Escherichia coli enzymologie MeSH
- kultivační média MeSH
- Proteus enzymologie MeSH
- střeva enzymologie mikrobiologie MeSH
- trypsin metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chymotrypsin MeSH
- kultivační média MeSH
- trypsin MeSH
