The aim of this study was to determine possible differences in the faecal microbiota of dairy cows infected with Mycobacterium avium subsp. paratuberculosis (Johne's disease) in comparison with noninfected cows from the same herds. Faecal samples from cows in 4 herds were tested for M. avium subsp. paratuberculosis by real-time PCR, and faecal bacterial populations were analysed by 454 pyrosequencing of the 16S rRNA gene. The most notable differences between shedding and nonshedding cows were an increase in the genus Psychrobacter and a decrease in the genera Oscillospira, Ruminococcus, and Bifidobacterium in cows infected with M. avium subsp. paratuberculosis. The present study is the first to report the faecal microbial composition in dairy cows infected with M. avium subsp. paratuberculosis.
- Klíčová slova
- MAP, Mycobacterium avium sous-espèce paratuberculosis, Mycobacterium avium subsp. paratuberculosis, bovin, cattle, composition microbienne fécale, faecal microbial composition, pyrosequencing, pyroséquençage, « MAP »,
- MeSH
- feces mikrobiologie MeSH
- mlékárenství MeSH
- Mycobacterium avium subsp. paratuberculosis genetika izolace a purifikace fyziologie MeSH
- nemoci skotu mikrobiologie MeSH
- paratuberkulóza mikrobiologie MeSH
- sekvenční analýza DNA veterinární MeSH
- skot mikrobiologie MeSH
- vylučování bakterií z těla MeSH
- zvířata MeSH
- Check Tag
- skot mikrobiologie MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
The aims of this study were to describe spatial contamination of the environment on a mouflon pasture, as well as to assess the contamination of grass and roots after surface contamination and in depth contamination with feces and buried tissues from animals infected with Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis). Samples of soil, roots, and aerial parts of plants were collected from different locations inside the mouflon pasture, and one control sample site was chosen outside the area where the animals are living. M. a. paratuberculosis DNA was present in all the examined sites and was more often detected in roots than in soil. DNA was detected at up to 80 cm of depth and was spatially more widespread than the initial hypothesis of M. a. paratuberculosis leaching vertically into deeper layers of soil. This study broadens our knowledge of the spread and persistence of M. a. paratuberculosis in an environment with highly infected animals.
- MeSH
- krmivo pro zvířata analýza mikrobiologie MeSH
- lipnicovité mikrobiologie MeSH
- Mycobacterium avium subsp. paratuberculosis genetika izolace a purifikace fyziologie MeSH
- nemoci skotu mikrobiologie přenos MeSH
- paratuberkulóza mikrobiologie přenos MeSH
- půda chemie MeSH
- půdní mikrobiologie * MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- půda MeSH
The syrphid Eristalis tenax Linnaeus (Diptera: Syrphidae) may be found in and around dung storage pits at cattle farms at various developmental stages of their life cycle. The purpose of this study was to investigate the occurrence of Mycobacterium avium ssp. paratuberculosis in 1044 E. tenax samples at various developmental stages, as well as fresh and stored dung originating from nine cattle farms. Mycobacterium fortuitum was isolated from one (1.5%) larva from the vicinity of three paratuberculosis-free herds of cattle. Mycobacterium a. paratuberculosis was isolated from 111 (21.4%) of E. tenax larvae collected from two of seven farms known to be infected with the causal agent of paratuberculosis. Mycobacteria were not isolated from any of the 340 pupae, 41 adults of 78 samples of exoskeletal exuviae. Mycobacterium a. paratuberculosis isolates from E. tenax larvae were of the IS900 restriction fragment length polymorphism (RFLP) type B-C1, identical to that detected in faecal samples from cattle herds infected with paratuberculosis. Larvae artificially infected with mycobacteria of IS900 RFLP type B-C9 did not contain statistically more CFU of identical IS900 RFLP type B-C9 in the intestinal tract and internal organs than on the body surface. These results show that M. a. paratuberculosis can survive in the intestinal tract and internal organs of E. tenax.
- MeSH
- Diptera mikrobiologie MeSH
- gastrointestinální trakt mikrobiologie MeSH
- hmyz - vektory mikrobiologie MeSH
- hnůj mikrobiologie MeSH
- hodnocení rizik MeSH
- larva mikrobiologie MeSH
- Mycobacterium avium subsp. paratuberculosis genetika fyziologie MeSH
- počet mikrobiálních kolonií MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- skot MeSH
- zemědělství MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Geografické názvy
- Česká republika MeSH
- Slovenská republika MeSH
- Názvy látek
- hnůj MeSH
