Titanium dioxide nanoparticles (TiO2NPs) are revolutionizing biomedicine due to their potential application as diagnostic and therapeutic agents. However, the TiO2NP immune-compatibility remains an open issue, even for ethical reasons. In this work, we investigated the immunomodulatory effects of TiO2NPs in an emergent proxy to human non-mammalian model for in vitro basic and translational immunology: the sea urchin Paracentrotus lividus. To highlight on the new insights into the evolutionarily conserved intracellular signaling and metabolism pathways involved in immune-TiO2NP recognition/interaction we applied a wide-ranging approach, including electron microscopy, biochemistry, transcriptomics and metabolomics. Findings highlight that TiO2NPs interact with immune cells suppressing the expression of genes encoding for proteins involved in immune response and apoptosis (e.g. NF-κB, FGFR2, JUN, MAPK14, FAS, VEGFR, Casp8), and boosting the immune cell antioxidant metabolic activity (e.g. pentose phosphate, cysteine-methionine, glycine-serine metabolism pathways). TiO2NP uptake was circumscribed to phagosomes/phagolysosomes, depicting harmless vesicular internalization. Our findings underlined that under TiO2NP-exposure sea urchin innate immune system is able to control inflammatory signaling, excite antioxidant metabolic activity and acquire immunological tolerance, providing a new level of understanding of the TiO2NP immune-compatibility that could be useful for the development in Nano medicines.

• Klíčová slova
• Homeostasis restoring, Human gene networks, Innate immunity, Metabolic rewiring, TiO(2)NP-responsive genes,
• MeSH
• antioxidancia metabolismus   MeSH
• chemické látky znečišťující vodu toxicita   MeSH
• fagocytóza účinky léků   MeSH
• genetická transkripce účinky léků   MeSH
• kultivované buňky MeSH
• nanočástice toxicita   MeSH
• Paracentrotus cytologie   účinky léků   imunologie   metabolismus   MeSH
• přirozená imunita účinky léků   genetika   MeSH
• titan toxicita   MeSH
• viabilita buněk účinky léků   imunologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• chemické látky znečišťující vodu MeSH
• titan MeSH
• titanium dioxide MeSH Prohlížeč