We report that the immunogenicity of colloidal gold nanoparticles coated with polyvinylpyrrolidone (PVP-AuNPs) in a model organism, the sea urchin Paracentrotus lividus, can function as a proxy for humans for in vitro immunological studies. To profile the immune recognition and interaction from exposure to PVP-AuNPs (1 and 10 μg mL-1), we applied an extensive nano-scale approach, including particle physicochemical characterisation involving immunology, cellular biology, and metabolomics. The interaction between PVP-AuNPs and soluble proteins of the sea urchin physiological coelomic fluid (blood equivalent) results in the formation of a protein "corona" surrounding the NPs from three major proteins that influence the hydrodynamic size and colloidal stability of the particle. At the lower concentration of PVP-AuNPs, the P. lividus phagocytes show a broad metabolic plasticity based on the biosynthesis of metabolites mediating inflammation and phagocytosis. At the higher concentration of PVP-AuNPs, phagocytes activate an immunological response involving Toll-like receptor 4 (TLR4) signalling pathway at 24 hours of exposure. These results emphasise that exposure to PVP-AuNPs drives inflammatory signalling by the phagocytes and the resolution at both the low and high concentrations of the PVP-AuNPs and provides more details regarding the immunogenicity of these NPs.
- Klíčová slova
- Immune metabolic rewiring, Immunoreactivity, Innate defence response, Nano-recognition, Sea urchin immune cells,
- MeSH
- fagocyty MeSH
- kovové nanočástice * toxicita MeSH
- lidé MeSH
- Paracentrotus * MeSH
- povidon MeSH
- zlato MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- povidon MeSH
- zlato MeSH
Titanium dioxide nanoparticles (TiO2NPs) are revolutionizing biomedicine due to their potential application as diagnostic and therapeutic agents. However, the TiO2NP immune-compatibility remains an open issue, even for ethical reasons. In this work, we investigated the immunomodulatory effects of TiO2NPs in an emergent proxy to human non-mammalian model for in vitro basic and translational immunology: the sea urchin Paracentrotus lividus. To highlight on the new insights into the evolutionarily conserved intracellular signaling and metabolism pathways involved in immune-TiO2NP recognition/interaction we applied a wide-ranging approach, including electron microscopy, biochemistry, transcriptomics and metabolomics. Findings highlight that TiO2NPs interact with immune cells suppressing the expression of genes encoding for proteins involved in immune response and apoptosis (e.g. NF-κB, FGFR2, JUN, MAPK14, FAS, VEGFR, Casp8), and boosting the immune cell antioxidant metabolic activity (e.g. pentose phosphate, cysteine-methionine, glycine-serine metabolism pathways). TiO2NP uptake was circumscribed to phagosomes/phagolysosomes, depicting harmless vesicular internalization. Our findings underlined that under TiO2NP-exposure sea urchin innate immune system is able to control inflammatory signaling, excite antioxidant metabolic activity and acquire immunological tolerance, providing a new level of understanding of the TiO2NP immune-compatibility that could be useful for the development in Nano medicines.
- Klíčová slova
- Homeostasis restoring, Human gene networks, Innate immunity, Metabolic rewiring, TiO(2)NP-responsive genes,
- MeSH
- antioxidancia metabolismus MeSH
- chemické látky znečišťující vodu toxicita MeSH
- fagocytóza účinky léků MeSH
- genetická transkripce účinky léků MeSH
- kultivované buňky MeSH
- nanočástice toxicita MeSH
- Paracentrotus cytologie účinky léků imunologie metabolismus MeSH
- přirozená imunita účinky léků genetika MeSH
- titan toxicita MeSH
- viabilita buněk účinky léků imunologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antioxidancia MeSH
- chemické látky znečišťující vodu MeSH
- titan MeSH
- titanium dioxide MeSH Prohlížeč
Extensive exploitation of titanium dioxide nanoparticles (TiO2NPs) augments rapid release into the marine environment. When in contact with the body fluids of marine invertebrates, TiO2NPs undergo a transformation and adhere various organic molecules that shape a complex protein corona prior to contacting cells and tissues. To elucidate the potential extracellular signals that may be involved in the particle recognition by immune cells of the sea urchin Paracentrotus lividus, we investigated the behavior of TiO2NPs in contact with extracellular proteins in vitro. Our findings indicate that TiO2NPs are able to interact with sea urchin proteins in both cell-free and cell-conditioned media. The two-dimensional proteome analysis of the protein corona bound to TiO2NP revealed that negatively charged proteins bound preferentially to the particles. The main constituents shaping the sea urchin cell-conditioned TiO2NP protein corona were proteins involved in cellular adhesion (Pl-toposome, Pl-galectin-8, Pl-nectin) and cytoskeletal organization (actin and tubulin). Immune cells (phagocytes) aggregated TiO2NPs on the outer cell surface and within well-organized vesicles without eliciting harmful effects on the biological activities of the cells. Cells showed an active metabolism, no oxidative stress or caspase activation. These results provide a new level of understanding of the extracellular proteins involved in the immune-TiO2NP recognition and interaction in vitro, confirming that primary immune cell cultures from P. lividus can be an optional model for swift and efficient immune-toxicological investigations.
- Klíčová slova
- biocorona, echinoderm, extracellular signaling, immune-adhesome, in vitro-ex vivo model, proxy to human,
- MeSH
- buněčná adheze imunologie MeSH
- fagocyty imunologie MeSH
- galektiny imunologie MeSH
- glykoproteiny imunologie MeSH
- ježovky imunologie MeSH
- nanočástice aplikace a dávkování MeSH
- nektiny imunologie MeSH
- Paracentrotus imunologie MeSH
- proteinová korona imunologie MeSH
- proteom imunologie MeSH
- titan imunologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- galektiny MeSH
- glykoproteiny MeSH
- nektiny MeSH
- proteinová korona MeSH
- proteom MeSH
- titan MeSH
- titanium dioxide MeSH Prohlížeč
- toposome glycoprotein complex MeSH Prohlížeč
Specimens of Syndesmis aethopharynx Westervelt & Kozloff, 1990 (Umagillidae, Rhabdocoela, Platyhelminthes) were collected from the intestine of several specimens of the sea urchin Paracentrotus lividus (Lamarck, 1816) [2], Hansson, 2001 at the Greek coast. This represents the first report of a species of Syndesmis from Greece. Our study has revealed several previously-unreported morphological details and intraspecific variation, which are added to the species description. The position of S. aethopharynx within Umagillidae is confirmed for the first time through molecular data (based on nuclear 18S rDNA), using both Bayesian and maximum likelihood analyses.
- Klíčová slova
- Echinoidea, Marine microturbellarians, Phylogeny, Taxonomy,
- MeSH
- DNA helmintů genetika MeSH
- fylogeneze MeSH
- interakce hostitele a parazita * MeSH
- Paracentrotus parazitologie MeSH
- RNA ribozomální 18S genetika MeSH
- rozšíření zvířat * MeSH
- Turbellaria anatomie a histologie genetika fyziologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Řecko MeSH
- Středozemní moře MeSH
- Názvy látek
- DNA helmintů MeSH
- RNA ribozomální 18S MeSH
