Titanium dioxide nanoparticles temporarily influence the sea urchin immunological state suppressing inflammatory-relate gene transcription and boosting antioxidant metabolic activity
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
31639584
DOI
10.1016/j.jhazmat.2019.121389
PII: S0304-3894(19)31343-3
Knihovny.cz E-resources
- Keywords
- Homeostasis restoring, Human gene networks, Innate immunity, Metabolic rewiring, TiO(2)NP-responsive genes,
- MeSH
- Antioxidants metabolism MeSH
- Water Pollutants, Chemical toxicity MeSH
- Phagocytosis drug effects MeSH
- Transcription, Genetic drug effects MeSH
- Cells, Cultured MeSH
- Nanoparticles toxicity MeSH
- Paracentrotus cytology drug effects immunology metabolism MeSH
- Immunity, Innate drug effects genetics MeSH
- Titanium toxicity MeSH
- Cell Survival drug effects immunology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antioxidants MeSH
- Water Pollutants, Chemical MeSH
- Titanium MeSH
- titanium dioxide MeSH Browser
Titanium dioxide nanoparticles (TiO2NPs) are revolutionizing biomedicine due to their potential application as diagnostic and therapeutic agents. However, the TiO2NP immune-compatibility remains an open issue, even for ethical reasons. In this work, we investigated the immunomodulatory effects of TiO2NPs in an emergent proxy to human non-mammalian model for in vitro basic and translational immunology: the sea urchin Paracentrotus lividus. To highlight on the new insights into the evolutionarily conserved intracellular signaling and metabolism pathways involved in immune-TiO2NP recognition/interaction we applied a wide-ranging approach, including electron microscopy, biochemistry, transcriptomics and metabolomics. Findings highlight that TiO2NPs interact with immune cells suppressing the expression of genes encoding for proteins involved in immune response and apoptosis (e.g. NF-κB, FGFR2, JUN, MAPK14, FAS, VEGFR, Casp8), and boosting the immune cell antioxidant metabolic activity (e.g. pentose phosphate, cysteine-methionine, glycine-serine metabolism pathways). TiO2NP uptake was circumscribed to phagosomes/phagolysosomes, depicting harmless vesicular internalization. Our findings underlined that under TiO2NP-exposure sea urchin innate immune system is able to control inflammatory signaling, excite antioxidant metabolic activity and acquire immunological tolerance, providing a new level of understanding of the TiO2NP immune-compatibility that could be useful for the development in Nano medicines.
Institute of Microbiology of The Czech Academy of Sciences Prague Czechia
Istituto per la Ricerca e l'Innovazione Biomedica Consiglio Nazionale delle Ricerche Palermo Italy
SYSBIO IT Centre of Systems Biology University of Milano Bicocca Milano Italy
References provided by Crossref.org
In Vitro Interactions of TiO2 Nanoparticles with Earthworm Coelomocytes: Immunotoxicity Assessment
DNA Methylation Profiles in a Group of Workers Occupationally Exposed to Nanoparticles
