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11 záznamů v PubMed Filtry

Článek

The energetic and metabolic effect of Ringerfundin (B. Braun) infusion and comparison with Plasma-Lyte (Baxter) in healthy volunteers

Acta medica (Hradec Kralove). 2010 ; 53 (3) : 131-7.

Acta Medica (Hradec Kralove)
ISSN 1211-4286
Zdroj

Measurement of parameters of energy requirement, respiratory quotient (RQ), rate of oxygen consumption (VO2) and rate of carbon dioxide production (VCO2) reveal Ringerfundin as an excellent and metabolically stable-acting balanced ionic solution, which does not increase the consumption of O2 or the total energy requirement. In conclusion, Ringerfundin was very well tolerated and in no case were observed undesirable effects.

MeSH
acetáty aplikace a dávkování farmakologie MeSH
chlorid draselný farmakologie MeSH
chlorid hořečnatý farmakologie MeSH
chlorid sodný farmakologie MeSH
dospělí MeSH
dýchání MeSH
elektrolyty aplikace a dávkování farmakologie MeSH
energetický metabolismus účinky léků MeSH
glukonáty farmakologie MeSH
intravenózní infuze MeSH
isotonické roztoky MeSH
lidé středního věku MeSH
lidé MeSH
mladiství MeSH
mladý dospělý MeSH
nepřímá kalorimetrie MeSH
octan sodný farmakologie MeSH
oxid uhličitý metabolismus MeSH
spotřeba kyslíku účinky léků MeSH
tekutinová terapie * MeSH
Check Tag
dospělí MeSH
lidé středního věku MeSH
lidé MeSH
mladiství MeSH
mladý dospělý MeSH
mužské pohlaví MeSH
ženské pohlaví MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH
randomizované kontrolované studie MeSH
srovnávací studie MeSH
Názvy látek
acetáty MeSH
chlorid draselný MeSH
chlorid hořečnatý MeSH
chlorid sodný MeSH
elektrolyty MeSH
glukonáty MeSH
isotonické roztoky MeSH
octan sodný MeSH
oxid uhličitý MeSH
Plasma-lyte 148 MeSH Prohlížeč
Ringerfundin MeSH Prohlížeč

Článek

The anti-inflammatory effects of inhaled corticosteroids versus anti-leukotrienes on the lymphocyte P-glycoprotein (PGP) expression in asthmatic children

The Journal of asthma. 2009 May ; 46 (4) : 366-70.

J Asthma
ISSN 1532-4303 | 0277-0903
Zdroj

BACKGROUND: Inhaled corticosteroids (ICS) are used in asthma therapy for their anti-inflammatory effects. P-glycoprotein (PGP) is a transmembrane efflux pump for many drugs, including corticosteroids. Expression of PGP is associated with therapy resistant disease. OBJECTIVE: The purpose of this study was to compare expression levels of PGP in blood lymphocytes of pediatric asthma patients either on ICS or on the leukotriene inhibitor montelukast medication. PATIENTS AND METHODS: The evaluation of lymphocyte PGP expression was performed on a sample of 99 children (66 boys and 33 girls) aged 2-18 (median 12) years with intermittent or persisting mild asthma, (as defined by GINY 2002). The asthmatic children were divided into 3 groups: (1) treated by ICS budesonide 200-400 microg per day (n = 27) more than 1 year; (2) treated by oral montelukast 4 or 5 mg at an age-based dose (n = 16); and (3) treated by inhaled corticosteroids and montelukast (n = 45). Reference PGP values were obtained from a group of 64 healthy children (23 boys and 29 girls) aged 2-15 years (median, 10 years). The expression of lymphocyte PGP was determined on fixed and permeabilized blood mononuclear cells using indirect immunofluorescence staining technique by flow cytometry modified according to Boer et al., 1997. RESULTS: Based on the weighted medians for PGP expression in peripheral blood lymphocytes, we found a significant difference (p < 10(-3)) between the group of asthma patients (n = 99), (619 +/- 5.3) and healthy controls (n = 64) (446.9 +/- 4.4). Second, there was a lower level of PGP expression in the group treated by ICS (548.6 +/- 9) than the group treated by montelukast and montelukast with budesonide (643.2 +/- 6.3) (p < 10(-6)). CONCLUSIONS: The anti-inflammatory activity of ICS is more effective in decreasing the production of pro-inflammatory mediators and results in reduced multidrug resistence (MDR-1) gene activity and expression of lymphocyte PGP in asthmatic children.

Článek

Percentage of eosinophils with surface CD44 and levels of eosinophil cationic protein in the peripheral blood in children with bronchial asthma and their changes after montelukast therapy

Allergy and asthma proceedings. 2006 Jul-Aug ; 27 (4) : 378-82.

Allergy Asthma Proc
ISSN 1088-5412
Zdroj

Bronchial asthma (BA) is chronic inflammation of the respiratory tract with a role played by a variety of cells, particularly mast cells, eosinophils (Eo), and T lymphocytes. The serum levels of Eo cationic protein (S-ECP) reflect the severity of bronchial inflammation and the level of bronchial hyperreactivity in asthma patients. One of the most important adhesion molecules is CD44. We examined S-ECP, the percentage of Eo with surface CD44 expression (EoCD44), and Eo count in the peripheral blood of newly diagnosed pediatric atopic patients with intermittent and persistent mild BA according to the Global Iniative for Asthma 2002, in a proportion of patients 3 months after initiation of montelukast therapy. Ninety-seven children with BA had their medical history taken, and S-ECP, with the percentage of EoCD44 determined by direct fluorescence from whole blood using flow cytometry with a Coulter EPICS XL cytometer, and Eo count, total serum immunoglobulin E levels (S-IgE) were determined. Therapy with montelukast (5 mg daily) was started in 23 children. Three months after the first collection, a second S-ECP level and EoCD44 count determinations were made. An inverse correlation between S-ECP and EoCD44 (-0.602; p < 0.0001) was found in the 97 children with BA. In the 23 children receiving montelukast we documented inverse correlation of fluctuation on S-ECP and EoCD44 after 3 months. These results were not significant. An inverse correlation between S-ECP and percent of EoCD44 was established in the 97 children with asthma before therapy initiation. The lower percentage of EoCD44 in peripheral blood in asthmatic children is due to Eo inflammation activity and attests to massive Eo invasion into the airways. Determination of the percentage proportion of EoCD44 is another potential indirect marker of the multiple features of Eo inflammation.

MeSH
acetáty aplikace a dávkování terapeutické užití MeSH
antagonisté leukotrienů aplikace a dávkování terapeutické užití MeSH
antigeny CD44 krev MeSH
bronchiální astma krev farmakoterapie MeSH
chinoliny aplikace a dávkování terapeutické užití MeSH
cyklopropany MeSH
dítě MeSH
eozinofilní kationtový protein krev MeSH
eozinofily MeSH
imunoglobulin E krev MeSH
lidé MeSH
mladiství MeSH
následné studie MeSH
počet leukocytů MeSH
předškolní dítě MeSH
rozvrh dávkování léků MeSH
sulfidy MeSH
Check Tag
dítě MeSH
lidé MeSH
mladiství MeSH
mužské pohlaví MeSH
předškolní dítě MeSH
ženské pohlaví MeSH
Publikační typ
časopisecké články MeSH
Názvy látek
acetáty MeSH
antagonisté leukotrienů MeSH
antigeny CD44 MeSH
chinoliny MeSH
cyklopropany MeSH
eozinofilní kationtový protein MeSH
imunoglobulin E MeSH
montelukast MeSH Prohlížeč
RNASE3 protein, human MeSH Prohlížeč
sulfidy MeSH

Článek

Montelukast decreases plasma endothelin-1 and serum eosinophil cationic protein levels in paediatric atopic asthma

Clinical drug investigation. 2006 ; 26 (6) : 351-6.

Clin Drug Investig
ISSN 1173-2563
Zdroj

BACKGROUND AND OBJECTIVE: Endothelin-1 (ET-1) is produced by vascular endothelial cells and epithelial cells, T-lymphocytes and phagocytes. Increased ET-1 levels have been demonstrated in the bronchial epithelium of asthma patients. In vitro, ET-1 stimulates mucus secretion, activates proinflammatory cells - macrophages and mast cells - and serves as a mitogenic stimulus for fibroblasts and smooth muscle. In addition, ET-1 activates phospholipase 2. Compared with healthy individuals, asthma patients have increased ET-1 levels during an attack and following stabilisation. Our study was designed to examine plasma ET-1 (P-ET) levels in paediatric atopic patients newly diagnosed with persistent mild bronchial asthma and 1 month after initiation of montelukast therapy. METHODS: Patients' histories were examined, and their blood eosinophil leucocyte count and levels of total serum immunoglobulin E (S-IgE), serum eosinophil cationic protein (S-ECP) and P-ET were determined. Thirty-six patients with persistent mild bronchial asthma were treated with the leukotriene receptor antagonist montelukast, administered once a day for 4 weeks. Second P-ET and S-ECP level determinations were made 4 weeks later with all the children included in the study. P-ET levels were also determined in a group of 27 healthy children who had no atopy in their medical histories and were taking no drugs (including montelukast), and who served as controls. RESULTS: The mean +/- SD pretreatment P-ET level in the 36 study children was 11.542 +/- 6.408 pg/L, and this decreased to 5.636 +/- 4.419 pg/L after 1 month's therapy with montelukast (statistically significant difference; p < 0.0001). Both of these values were significantly higher (p < 0.0001 and p < 0.031, respectively) than the mean level in the control group of 27 children (3.543 +/- 2.497 pg/L). The mean pretreatment S-ECP level was 35.78 +/- 19.58 microg/L, and this decreased to 19.54 +/- 13.86 microg/L after 1 month's therapy (p < 0.001). CONCLUSIONS: This study demonstrated a decrease in P-ET levels in children with mild asthma receiving montelukast. This indicates a reduction in the severity of the inflammatory response and, hence, provides evidence for the anti-inflammatory effect of montelukast. Monitoring both ET-1 and ECP levels at regular follow-up may be useful in assessing these two facets of activity of chronic inflammation in bronchial asthma.

Článek

Treatment of chronic cough in children with montelukast,a leukotriene receptor antagonist

The Journal of asthma. 2004 Oct ; 41 (7) : 715-20.

J Asthma
ISSN 0277-0903
Zdroj

Twenty-two children (13 boys and 9 girls) with chronic cough were treated with the leukotriene receptor antagonist montelukast (Singulair tbl. 5 mg) administered once daily for four weeks. In 14 children (68%), the cough ceased during the third week of treatment. Children responding to montelukast were found to have higher blood levels of eosinophil cationic protein (S-ECP) in the pretreatment blood sample than children with no response (responders 14.88+/-2.651 microg/l versus nonresponders 6.62+/-0.948 microg/l; p<0.01). Blood S-ECP levels remained higher also in the post-treatment blood sample in responders (10.55+/-1.631 microg/l) compared to nonresponders (6.13+/-0.937 microg/l; p<0.05). The difference is statistically significant. There were also differences in absolute eosinophil blood count and IgE blood levels between the two groups in the pretreatment blood sample. Using 24-hour pH-metry, two children not responding to therapy were subsequently diagnosed to have gastroesophageal reflux. Judging from the results, one might deduct that patients with chronic cough who have increased levels of serum ECP and absolute eosinophil blood counts are likely to benefit from treatment with montelukast.

MeSH
acetáty aplikace a dávkování MeSH
antagonisté leukotrienů aplikace a dávkování MeSH
biologické markery analýza MeSH
chinoliny aplikace a dávkování MeSH
chronická nemoc MeSH
cyklopropany MeSH
eozinofilní kationtový protein analýza účinky léků MeSH
eozinofily účinky léků MeSH
hodnocení rizik MeSH
imunoglobulin E analýza účinky léků MeSH
kašel diagnóza farmakoterapie MeSH
lidé MeSH
následné studie MeSH
pravděpodobnost MeSH
prospektivní studie MeSH
rozvrh dávkování léků MeSH
stupeň závažnosti nemoci MeSH
sulfidy MeSH
výsledek terapie MeSH
vztah mezi dávkou a účinkem léčiva MeSH
Check Tag
lidé MeSH
mužské pohlaví MeSH
ženské pohlaví MeSH
Publikační typ
časopisecké články MeSH
srovnávací studie MeSH
Názvy látek
acetáty MeSH
antagonisté leukotrienů MeSH
biologické markery MeSH
chinoliny MeSH
cyklopropany MeSH
eozinofilní kationtový protein MeSH
imunoglobulin E MeSH
montelukast MeSH Prohlížeč
sulfidy MeSH

Článek

The effect of ammonia and pH on brain gamma-glutamyl transpeptidase in young rats

FEBS letters. 1992 Apr 06 ; 300 (3) : 247-50.

FEBS Lett
ISSN 0014-5793
Zdroj

Acute hyperammonemia, induced by two consecutive injections of ammonium acetate (550 and 450 mg per kg b.wt.), decreased the activity of gamma-glutamyl transpeptidase (GGT) in most brain regions of 18- and 30-day-old rats. This decrease in the brain GGT activity was more pronounced in younger than in older rats. After the addition of NH4Cl to the incubation medium, the inhibitory action of NH4+ on this enzyme activity was also demonstrated in crude synaptosomal membranes at pH 7.4, but in a range of NH4+ concentrations many-times higher than those found in the plasma or brains of young hyperammonemic rats. Because similar concentrations of NH4+ stimulated the activity of the purified enzyme from rat kidney (mainly at pH 9.0), the inhibition of GGT activity in the young rat brain is probably mediated indirectly and not by a direct interaction of ammonia with the enzyme molecules.

MeSH
acetáty aplikace a dávkování MeSH
amoniak krev farmakologie MeSH
gama-glutamyltransferasa antagonisté a inhibitory metabolismus MeSH
inbrední kmeny potkanů MeSH
koncentrace vodíkových iontů MeSH
krysa rodu Rattus MeSH
ledviny enzymologie MeSH
molekulární sekvence - údaje MeSH
mozek účinky léků enzymologie růst a vývoj MeSH
novorozená zvířata MeSH
rozvrh dávkování léků MeSH
synaptozomy enzymologie MeSH
zvířata MeSH
Check Tag
krysa rodu Rattus MeSH
ženské pohlaví MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH
srovnávací studie MeSH
Názvy látek
acetáty MeSH
ammonium acetate MeSH Prohlížeč
amoniak MeSH
gama-glutamyltransferasa MeSH

Článek

Pouzití octanu sodného v krmných dávkách ketózních krav
[Use of sodium acetate in feed rations in ketotic cows]

Veterinarni medicina. 1989 Oct ; 34 (10) : 585-92.

Vet Med (Praha)
ISSN 0375-8427
Zdroj

On a farm where the ration of cows contained 88.0 g of butyric acid, an experimental group of cows (n = 8), producing milk containing 7.9 mg or more acetone per litre, was studied for 14 days for the effect of a 250 g supplement of sodium acetate to the ration (combined with single i.m. administration of vitamins A, D2 and E) on selected metabolism parameters and on milk production. As distinct from the control group of cows (n = 8) from the same farm which produced milk containing 3.9 mg or less acetone per litre and which were fed without sodium acetone supplements, a tendency of increased alkaemia of the organism was suggested in the experimental cows. This tendency manifested itself during the trial in increased pH values, increased base excess (BE) and standard bicarbonate (SB) in the blood, and in an increase in the pH value and net acido basic secretion in urine. A decrease was recorded in the concentration of the acetone + acetacetic acid sum, the same as beta-hydroxybutyric acid in blood and the sum of acetone and acetacetic acid in milk (P less than 0.01). An insignificant increase of the activity of gammaglutamyl transpeptidase (GMT) was recorded in the blood serum of the experimental cows and a significant increase occurred in the content of potassium (up to P less than 0.01) and urea (up to P less than 0.01) in urine. The supplement of sodium acetate to the feed ration did not influence the degree of ketonuria and the finding of urobilinogen in urine.(ABSTRACT TRUNCATED AT 250 WORDS)