BACKGROUND: S100(+) dendritic cells and CD57(+) lymphocytes are factors reflecting the immune system's ability to suppress the progress of tumor growth. CD57(+) cells include natural killer cells and late stages of T-effector lymphocytes. We evaluated the relationship between the known clinical and histological factors and tumor markers as well as the presence of S100(+) and CD57(+) cells in the tissue of colorectal carcinoma with the aim of detecting patients at high risk of short overall survival (OS) or short disease-free interval (DFI) after radical surgical treatment and we further analyzed whether S100(+) and CD57(+) positivity could bring on new information regarding the treatment regimen. MATERIALS AND METHODS: Data of 150 patients (97 males and 53 females) that underwent an elective radical surgical procedure for colorectal cancer were studied. The influence on DFI and on OS of the following parameters was evaluated: grading, staging and positivity for S100 and CD57 by immunohistochemical staining. We also analyzed the relation of preoperative serum levels of the tumor markers Carcinoembryonic Antigen (CEA), Cancer Antigen 19-9 (CA19-9), Cancer Antigen 72-4 (CA72-4), Thymidine kinase (TK), Tissue-Specific Polypeptide Antigen (TPS) and Tissue Polypeptide Antigen (TPA) in relation to S100 and CD57 positivity/negativity for the same patients. RESULTS: OS at 1, 3 and 5 years was 92.2%, 76.5% and 70.2%; the DFI at 1, 3 and 5 years was 85.3%, 64.3% and 49.4%. CD57 positivity in the tumor mass was proven as a positive prognostic factor for OS. Risk of short OS was 2.5-fold higher in patients with low tumor infiltration by CD57(+) lymphocytes. The combination of N2 stage for lymph nodes and the absence of CD57(+) cells was proven to be the strongest negative prognostic factor for OS. No significant influence of CD57 positivity on DFI appeared. There was no significant influence of S100 positivity on OS or DFI; nor was there any statistical dependence of CD57 and S100 positivity or negativity on preoperative serum levels of CEA, CA19-9, CA72-4, TK, TPS or TPA. Both studied factors were shown to be statistically independent factors. CONCLUSION: The present study showed infiltration of colorectal cancer tissue by CD57(+) cells as being an important independent positive prognostic factor for OS.

• MeSH
• antigeny CD57 analýza   MeSH
• dendritické buňky imunologie   MeSH
• kolorektální nádory chemie   imunologie   patologie   chirurgie   MeSH
• lidé MeSH
• nádorové biomarkery krev   MeSH
• prognóza MeSH
• proteiny S100 analýza   MeSH
• senioři MeSH
• staging nádorů MeSH
• T-lymfocyty imunologie   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny CD57 MeSH
• nádorové biomarkery MeSH
• proteiny S100 MeSH

Dendritic cell neurofibroma with pseudorosettes (DCNWPR) is a recently proposed variant of neurofibroma. However, its peripheral nerve sheath origin has subsequently been questioned, and it has been suggested that the neoplasm could represent a hitherto undescribed variant of melanocytic nevus with neural differentiation. Here we report a case of DCNWPR that arose almost exclusively within the confinement of the perineurium in the skin. This observation gives further evidence that this entity is a peripheral nerve sheath tumor and is unrelated to melanocytic neoplasms.

• MeSH
• antigeny CD57 analýza   MeSH
• dendritické buňky chemie   patologie   MeSH
• imunohistochemie MeSH
• lidé středního věku MeSH
• lidé MeSH
• nádorové biomarkery analýza   MeSH
• nádory nervové pochvy chemie   patologie   MeSH
• neurofibrom chemie   patologie   MeSH
• periferní nervy chemie   patologie   MeSH
• proteiny S100 analýza   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• Názvy látek
• antigeny CD57 MeSH
• nádorové biomarkery MeSH
• proteiny S100 MeSH

Thirty-seven carcinoids of the gastrointestinal tract were studied with immunohistochemical staining for chromogranin A (CgA) and Leu-7 (CD57). The aim of this study was to distinguish and describe the differences in patterns of distribution of immunostaining of these two non-specific neuroendocrine markers in neuroendocrine tumors of different degree of differentiation (typical, vs. atypical carcinoids) at different gastrointestinal sites. Selected 5 tumors from this group were studied in detail using confocal laser scanning microscopy (CLSM) and double immunofluorescence staining to disclose the patterns of distribution of CgA and CD57 positive granules within the individual tumor cells. Prominent differences in the patterns of immunohistochemical staining for both studied markers related to the degree of differentiation of the tumors were observed in studied neoplasms. Regular (diffuse) strongly positive immunoreaction for CgA predominated in typical carcinoids, whereas atypical tumors were characterized by irregular patchy staining. Both typical and atypical tumors displayed predominantly irregular patchy staining for CD57. The results of CLSM study indicate that different modes of CgA and CD57 expression and/or co-expression can occur in neuroendocrine tumors. Neoplastic cells that contained either CgA positive neuroendocrine granules (NEG), or Leu-7 positive NEG, were frequently observed in different areas of the tumor samples, especially in atypical carcinoids. Varying number of cells revealed co-localisation of both CgA and Leu-7 within the NEG. Similar co-localisation of CgA and CD57 was found in non-neoplastic Kultschitski cells of the mucosa of small intestine. In conclusion, our results suggest that the differences in CgA and CD57 expression in human neuroendocrine tumors are related to the degree of differentiation of the neoplasms and probably reflect the degree of maturation (functional state) of neuroendocrine granules within the neoplastic cells.

• MeSH
• antigeny CD57 analýza   MeSH
• chromogranin A MeSH
• chromograniny analýza   MeSH
• gastrointestinální nádory metabolismus   patologie   MeSH
• imunohistochemie MeSH
• karcinoid metabolismus   patologie   MeSH
• konfokální mikroskopie MeSH
• lidé MeSH
• nádorové biomarkery MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• antigeny CD57 MeSH
• CHGA protein, human MeSH Prohlížeč
• chromogranin A MeSH
• chromograniny MeSH
• nádorové biomarkery MeSH

The group of 35 carcinoid tumours obtained from 34 patients was reviewed according to recent histopathological criteria. Consequently, evaluation of the Grimelius staining and immunohistochemical detection of chromogranin A (CgA), Leu-7 (CD-57), synaptophysin, neuron-specific enolase (NSE), (beta-III tubulin, Ki-67 and proliferating cell nuclear antigen (PCNA) was performed. The majority of tumours (29, i.e. 83%) were classified as typical carcinoids composed predominantly of mixed solid and trabecular or solid and tubular growth patterns. Six tumours (17%) revealed more prominent cytological abnormalities corresponding with the diagnosis of atypical carcinoid. The majority of tumours (31, i.e. 93.9%) showed granular cytoplasmic positivity in Grimelius staining and diffuse cytoplasmic positivity of NSE (34, i.e. 97.1%). All of the 32 stained tumour samples showed positive immunoreactivity for synaptophysin. A high percentage of tumours (32, i.e. 91.4%) revealed also a positive reaction with antibody TU-20 detecting (beta-III tubulin, a marker of an early stage of neuronal differentiation. Thirty-four tumours (97.1%) showed granular cytoplasmic positivity for both markers of neuroendocrine granules (CgA and Leu-7). One tumour (2.9%) was positive only for Leu-7. Tumour cells revealed predominantly low proliferative activity evaluated by PCNA and Ki-67 immunodetection. Higher degree of proliferation was observed especially in atypical carcinoids.

• MeSH
• antigen Ki-67 analýza   MeSH
• antigeny CD57 analýza   MeSH
• chromogranin A MeSH
• chromograniny analýza   MeSH
• dospělí MeSH
• fosfopyruváthydratasa analýza   MeSH
• gastrointestinální nádory chemie   diagnóza   MeSH
• imunohistochemie MeSH
• karcinoid chemie   diagnóza   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• nádorové biomarkery analýza   MeSH
• proliferační antigen buněčného jádra analýza   MeSH
• senioři MeSH
• synaptofysin analýza   MeSH
• tubulin analýza   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigen Ki-67 MeSH
• antigeny CD57 MeSH
• chromogranin A MeSH
• chromograniny MeSH
• fosfopyruváthydratasa MeSH
• nádorové biomarkery MeSH
• proliferační antigen buněčného jádra MeSH
• synaptofysin MeSH
• tubulin MeSH

Alcian blue pH 2.5 and Leu 7 reactivities were tested in biopsy samples of thyroid neoplastic and non-neoplastic diseases (n = 30). Typical papillary carcinomas exhibited marked positivity in the apical zone of thyrocytes, but the negativity of most cases of follicular variants of papillary carcinoma and positivity found in the toxic goitres and exceptionally also in the follicular adenoma makes alcian blue of nearly no value for the differential diagnosis on cytodiagnostic level. The Leu 7 immunohistochemistry was specific for papillary carcinomas but with a very low sensitivity. Thus, its cytodiagnostic use would be not cost effective.

• MeSH
• alciánová modř * MeSH
• antigeny CD57 analýza   MeSH
• barvení a značení * MeSH
• diferenciální diagnóza MeSH
• imunohistochemie MeSH
• lidé MeSH
• nádorové biomarkery analýza   MeSH
• nádory štítné žlázy diagnóza   MeSH
• papilární karcinom diagnóza   MeSH
• senzitivita a specificita MeSH
• struma diagnóza   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• alciánová modř * MeSH
• antigeny CD57 MeSH
• nádorové biomarkery MeSH