Rous sarcoma virus-33 (RSV-33) was obtained from a sample of chicken Rous sarcoma which had been dried and stored in 1933. RSV-33, like the RSV-29, has the minimal number of passages beyond its isolation from chicken tumour No. 1. Our experiments demonstrated that the Rous sarcoma virus-33 was replication non-defective and was pathogenic for rats. Established rat tumorigenic cell lines express the viral genome. All three species of viral RNA were detected and v-src proteins and gag polyproteins were identified as well in cells of R9 and R74 lines. The virus can be rescued from cells of R9 and R74 lines, thus indicating that the cells are virogenic. The cells of a permanent tumorigenic line RT1 are infected but not transformed by RSV-33. Although they contain a complete proviral genome, they do not express detectable virus-specific RNA. The virus is not rescuable from RT1 cells under in vivo conditions. Proviral DNA analysis showed that the RSV-33 contained a full-length genome, including the env gene, in contrast to the RSV-29 which was found replication defective.

• MeSH
• buněčné linie MeSH
• chromozomy MeSH
• experimentální sarkom chemie   mikrobiologie   patologie   MeSH
• genom virový MeSH
• krysa rodu Rattus MeSH
• kur domácí MeSH
• nádorové buňky kultivované MeSH
• potkani inbrední LEW MeSH
• proviry genetika   MeSH
• virové proteiny metabolismus   MeSH
• viry ptačího sarkomu růst a vývoj   patogenita   fyziologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• virové proteiny MeSH

The cryptovirogenic PR-RSH-19 (H-19) hamster cell line, derived originally from a tumour induced by the virus rescued from XC cells by transfection, was studied. The pp60v-src protein was detected by immunoprecipitation with sera from tumour-bearing rabbits (TBR sera) from lysates of H-19 cells. A closely related protein p60 was precipitated by antiserum obtained from hamsters with tumours induced by H-19 cells. The pp60v-src of H-19 cells possesses an associated kinase activity. These results are consistent with the notion that the detected pp60v-src protein is a product of the cryptic sequences in H-19 cells.

• MeSH
• buněčné linie MeSH
• experimentální sarkom analýza   mikrobiologie   MeSH
• králíci MeSH
• křečci praví MeSH
• křeček rodu Mesocricetus MeSH
• kuřecí embryo MeSH
• nádorové proteiny imunologie   izolace a purifikace   MeSH
• onkogenní protein pp60(v-src) MeSH
• proteinkinasy izolace a purifikace   MeSH
• protilátky nádorové imunologie   MeSH
• virová transformace buněk * MeSH
• virové proteiny imunologie   izolace a purifikace   MeSH
• viry ptačího sarkomu MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• křečci praví MeSH
• kuřecí embryo MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• nádorové proteiny MeSH
• onkogenní protein pp60(v-src) MeSH
• proteinkinasy MeSH
• protilátky nádorové MeSH
• virové proteiny MeSH

• MeSH
• cysty mikrobiologie   MeSH
• druhová specificita MeSH
• experimentální leukemie mikrobiologie   MeSH
• experimentální sarkom mikrobiologie   MeSH
• hadi * MeSH
• injekce MeSH
• ještěři * MeSH
• kur domácí MeSH
• lidé MeSH
• transfuze leukocytů MeSH
• transplantace heterologní MeSH
• Urodela * MeSH
• viry ptačího sarkomu patogenita   MeSH
• želvy * MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH