The bacterial strain KDF8 capable of growth in the presence of diclofenac and codeine analgesics was obtained after chemical mutagenesis of nature isolates from polluted soils. The strain KDF8 was identified as Raoultella sp. based on its morphology, biochemical properties, and 16S rRNA gene sequence. It was deposited in the Czech Collection of Microorganisms under the number CCM 8678. A growing culture efficiently removed diclofenac (92% removal) and partially also codeine (about 30% degradation) from culture supernatants within 72 h at 28 °C. The degradation of six analgesics by the whole cell catalyst was investigated in detail. The maximum degradation of diclofenac (91%) by the catalyst was achieved at pHINI of 7 (1 g/L diclofenac). The specific removal rate at high concentrations of diclofenac and codeine increased up to 16.5 mg/gCDW per h and 5.1 mg/gCDW per h, respectively. HPLC analysis identified 4'-hydroxydiclofenac as a major metabolite of diclofenac transformation and 14-hydroxycodeinone as codeine transformation product. The analgesics ibuprofen and ketoprofen were also removed, albeit to a lower extent of 3.2 and 2.0 mg/gCDW per h, respectively. Naproxen and mefenamic acid were not degraded.
- Klíčová slova
- Analgesics, Co-metabolism, Microbial degradation, Raoultella sp., Whole cell catalyst,
- MeSH
- analgetika metabolismus toxicita MeSH
- chemické látky znečišťující vodu metabolismus MeSH
- diklofenak metabolismus toxicita MeSH
- DNA bakterií genetika MeSH
- Enterobacteriaceae klasifikace účinky léků metabolismus MeSH
- fylogeneze MeSH
- kodein metabolismus toxicita MeSH
- koncentrace vodíkových iontů MeSH
- mikrobiální viabilita účinky léků MeSH
- půdní mikrobiologie MeSH
- RNA ribozomální 16S genetika MeSH
- teplota MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- analgetika MeSH
- chemické látky znečišťující vodu MeSH
- diklofenak MeSH
- DNA bakterií MeSH
- kodein MeSH
- RNA ribozomální 16S MeSH
- MeSH
- kodein analogy a deriváty metabolismus účinky záření MeSH
- krysa rodu Rattus MeSH
- morfin metabolismus účinky záření MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kodein MeSH
- morfin MeSH
