Trypanosoma brucei has a complex life cycle during which its single mitochondrion is subjected to major metabolic and morphological changes. While the procyclic stage (PS) of the insect vector contains a large and reticulated mitochondrion, its counterpart in the bloodstream stage (BS) parasitizing mammals is highly reduced and seems to be devoid of most functions. We show here that key Fe-S cluster assembly proteins are still present and active in this organelle and that produced clusters are incorporated into overexpressed enzymes. Importantly, the cysteine desulfurase Nfs, equipped with the nuclear localization signal, was detected in the nucleolus of both T. brucei life stages. The scaffold protein Isu, an interacting partner of Nfs, was also found to have a dual localization in the mitochondrion and the nucleolus, while frataxin and both ferredoxins are confined to the mitochondrion. Moreover, upon depletion of Isu, cytosolic tRNA thiolation dropped in the PS but not BS parasites.
- MeSH
- aktivní transport - buněčné jádro MeSH
- buněčné jádro metabolismus MeSH
- ferredoxiny metabolismus MeSH
- frataxin MeSH
- jaderné lokalizační signály MeSH
- lyasy štěpící vazby C-S chemie genetika metabolismus MeSH
- mitochondriální proteiny metabolismus MeSH
- mitochondrie metabolismus MeSH
- molekulární sekvence - údaje MeSH
- multimerizace proteinu MeSH
- proteiny asociované s jadernou matrix chemie genetika metabolismus MeSH
- proteiny vázající železo metabolismus MeSH
- protozoální proteiny chemie genetika metabolismus MeSH
- sekvence aminokyselin MeSH
- Trypanosoma brucei brucei enzymologie genetika metabolismus MeSH
- vazba proteinů MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cysteine desulfurase MeSH Prohlížeč
- ferredoxiny MeSH
- jaderné lokalizační signály MeSH
- lyasy štěpící vazby C-S MeSH
- mitochondriální proteiny MeSH
- proteiny asociované s jadernou matrix MeSH
- proteiny vázající železo MeSH
- protozoální proteiny MeSH
