Escherichia coli strain which contains a marker of tetracycline resistance gene (TcR) placed by P1 transduction beside the chromosomal deletion of ampC gene (delta ampC) coding for beta-lactamase was constructed. Such introduction of TcR marker permits a fast and simple selection for the transfer of delta ampC by P1 transduction into industrial E. coli strains. This approach was used for constructing an E. coli strain suitable for penicillin acylase production.

• MeSH
• Genes, Bacterial * MeSH
• Bacterial Proteins * MeSH
• Bacteriophage P1 genetics   MeSH
• beta-Lactamases genetics   MeSH
• Gene Deletion MeSH
• Escherichia coli drug effects   enzymology   genetics   MeSH
• Genetic Markers MeSH
• Penicillin Amidase biosynthesis   genetics   MeSH
• Plasmids genetics   MeSH
• Recombinant Proteins biosynthesis   genetics   MeSH
• Tetracycline Resistance genetics   MeSH
• Transduction, Genetic MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• AmpC beta-lactamases MeSH Browser
• Bacterial Proteins * MeSH
• beta-Lactamases MeSH
• Genetic Markers MeSH
• Penicillin Amidase MeSH
• Recombinant Proteins MeSH