Plants regularly face the changing climatic conditions that cause biotic and abiotic stress responses. The abiotic stresses are the primary constraints affecting crop yield and nutritional quality in many crop plants. The advances in genome sequencing and high-throughput approaches have enabled the researchers to use genome editing tools for the functional characterization of many genes useful for crop improvement. The present review focuses on the genome editing tools for improving many traits such as disease resistance, abiotic stress tolerance, yield, quality, and nutritional aspects of tomato. Many candidate genes conferring tolerance to abiotic stresses such as heat, cold, drought, and salinity stress have been successfully manipulated by gene modification and editing techniques such as RNA interference, insertional mutagenesis, and clustered regularly interspaced short palindromic repeat (CRISPR/Cas9). In this regard, the genome editing tools such as CRISPR/Cas9, which is a fast and efficient technology that can be exploited to explore the genetic resources for the improvement of tomato and other crop plants in terms of stress tolerance and nutritional quality. The review presents examples of gene editing responsible for conferring both biotic and abiotic stresses in tomato simultaneously. The literature on using this powerful technology to improve fruit quality, yield, and nutritional aspects in tomato is highlighted. Finally, the prospects and challenges of genome editing, public and political acceptance in tomato are discussed.

• Klíčová slova
• abiotic stress, biotic stress, gene knockout, resistance breeding, trait improvement,
• MeSH
• CRISPR-Cas systémy MeSH
• editace genu * MeSH
• epigenomika metody   MeSH
• geneticky modifikované rostliny MeSH
• genom rostlinný * MeSH
• genomika * metody   MeSH
• genový knockdown MeSH
• mutageneze MeSH
• oxidační stres MeSH
• šlechtění rostlin * MeSH
• Solanum lycopersicum genetika   MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH