Nejvíce citovaný článek - PubMed ID 10664888
Isolation and characterization of bacteria degrading polychlorinated biphenyls from transformer oil
Biphenyl-utilizing polychlorinated biphenyls (PCB)-degrading bacteria were isolated from sites highly contaminated by PCBs, and their degradation abilities were determined using GC for typical commercial PCB mixtures (Delor 103 and Delor 106). Out of twelve strains which utilized biphenyl as a sole source of carbon and energy, strains Pseudomonas alcaligenes KP2 and P. fluorescens KP12, characterized by the BIOLOG identification system and the NEFERM test, were shown to significantly co-metabolize the PCB mixture Delor 103. DNA-DNA hybridization was used to compare both strains with well-known PCB-degraders Burkholderia cepacia strain LB400 and Ralstonia eutropha strain H850. The strain KP12 employs the same meta-fission route for degradation of chlorobenzoates as a chlorobiphenyl degrader Pseudomonas cepacia P166. Both isolates KP2 and KP12 belong to different phylogenetic groups, which indicates that the same geographical location does not ensure the same ancestor of degradative enzymes. We confirmed that also highly chlorinated and the most toxic congeners, which are contained in commercial PCB mixtures, can be biotransformed by members of indigenous bacterial-soil community under aerobic conditions.
- MeSH
- biodegradace MeSH
- Burkholderia cepacia izolace a purifikace metabolismus MeSH
- Cupriavidus necator izolace a purifikace metabolismus MeSH
- DNA bakterií chemie izolace a purifikace MeSH
- látky znečišťující půdu metabolismus toxicita MeSH
- polychlorované bifenyly metabolismus MeSH
- Pseudomonas genetika izolace a purifikace metabolismus MeSH
- půdní mikrobiologie * MeSH
- Southernův blotting MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- DNA bakterií MeSH
- látky znečišťující půdu MeSH
- polychlorované bifenyly MeSH
Moderately halophilic bacteria utilizing phenol as the sole carbon source were isolated by selective enrichment from sea water. The isolate (Gram-negative motile rods) was identified as Deleya venusta. It grew well in the presence of up to 1600 mg/L of phenol and 8% NaCl under aerobic conditions. When the cells were treated with chloramphenicol prior to the addition of phenol they did not utilize added phenol, even after prolonged incubation. Thus, the enzymes necessary for phenol metabolism appeared to be inducible.
- MeSH
- Arthrobacter klasifikace růst a vývoj izolace a purifikace MeSH
- Bacteria klasifikace růst a vývoj izolace a purifikace MeSH
- bakteriologické techniky MeSH
- biodegradace MeSH
- chemické látky znečišťující vodu metabolismus MeSH
- chlorid sodný MeSH
- fenol metabolismus MeSH
- Halomonas klasifikace růst a vývoj izolace a purifikace MeSH
- kultivační média MeSH
- mořská voda mikrobiologie MeSH
- Vibrio klasifikace růst a vývoj izolace a purifikace MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- Názvy látek
- chemické látky znečišťující vodu MeSH
- chlorid sodný MeSH
- fenol MeSH
- kultivační média MeSH
Two oil-degrading bacteria identified as Pseudomonas aeruginosa and Micrococcus luteus were isolated from crude-oil-polluted soils in Nigeria. The organisms were grown on n-hexadecane and sodium succinate and then examined for the presence of hydrocarbon inclusions. Inclusion bodies were found in n-hexadecane-grown cells and were absent in succinate-grown cells. Formation of hydrocarbon inclusion bodies appears to be a general phenomenon among hydrocarbon utilizers.
- MeSH
- alkany metabolismus MeSH
- buněčná inkluze ultrastruktura MeSH
- kultivační média MeSH
- látky znečišťující půdu * MeSH
- Micrococcus luteus izolace a purifikace metabolismus ultrastruktura MeSH
- Pseudomonas aeruginosa izolace a purifikace metabolismus ultrastruktura MeSH
- půdní mikrobiologie * MeSH
- ropa metabolismus MeSH
- sukcináty MeSH
- tropické klima MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alkany MeSH
- kultivační média MeSH
- látky znečišťující půdu * MeSH
- n-hexadecane MeSH Prohlížeč
- ropa MeSH
- sukcináty MeSH
A strain of bacteria able to detoxicate the herbicide diuron in pure culture was isolated from sites contaminated with different urea herbicides. Diuron was used as a sole source of carbon and energy by this isolate which is a Gram-negative, aerobic, rod-shaped bacterium with a single polar flagellum, and grows at 40 degrees C. The strain has been identified as Pseudomonas sp.
- MeSH
- biodegradace MeSH
- diuron metabolismus MeSH
- herbicidy metabolismus MeSH
- kultivační média MeSH
- látky znečišťující půdu metabolismus MeSH
- Pseudomonas růst a vývoj izolace a purifikace metabolismus MeSH
- půdní mikrobiologie * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- diuron MeSH
- herbicidy MeSH
- kultivační média MeSH
- látky znečišťující půdu MeSH
Spread-plating belongs to traditional microbiological methods employed for quantification of subsurface microflora during bioremediation projects in the Czechia. Concentration of degrading organisms is estimated from the number of colonies grown on agar plates supplied with contaminant as the sole carbon source. The data obtained during in situ bioremediation of the Dacice site contaminated by cutting oil suggests that changes in the composition of the carbon source in the subsurface may cause a discrepancy between laboratory data and situation in subsurface.
- MeSH
- Bacteria izolace a purifikace metabolismus MeSH
- bakteriologické techniky * MeSH
- biodegradace MeSH
- kultivační média MeSH
- mikrobiologie životního prostředí * MeSH
- oleje metabolismus MeSH
- uhlík MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- kultivační média MeSH
- oleje MeSH
- uhlík MeSH