Biphenyl-utilizing polychlorinated biphenyls (PCB)-degrading bacteria were isolated from sites highly contaminated by PCBs, and their degradation abilities were determined using GC for typical commercial PCB mixtures (Delor 103 and Delor 106). Out of twelve strains which utilized biphenyl as a sole source of carbon and energy, strains Pseudomonas alcaligenes KP2 and P. fluorescens KP12, characterized by the BIOLOG identification system and the NEFERM test, were shown to significantly co-metabolize the PCB mixture Delor 103. DNA-DNA hybridization was used to compare both strains with well-known PCB-degraders Burkholderia cepacia strain LB400 and Ralstonia eutropha strain H850. The strain KP12 employs the same meta-fission route for degradation of chlorobenzoates as a chlorobiphenyl degrader Pseudomonas cepacia P166. Both isolates KP2 and KP12 belong to different phylogenetic groups, which indicates that the same geographical location does not ensure the same ancestor of degradative enzymes. We confirmed that also highly chlorinated and the most toxic congeners, which are contained in commercial PCB mixtures, can be biotransformed by members of indigenous bacterial-soil community under aerobic conditions.

• MeSH
• Biodegradation, Environmental MeSH
• Burkholderia cepacia isolation & purification   metabolism   MeSH
• Cupriavidus necator isolation & purification   metabolism   MeSH
• DNA, Bacterial chemistry   isolation & purification   MeSH
• Soil Pollutants metabolism   toxicity   MeSH
• Polychlorinated Biphenyls metabolism   MeSH
• Pseudomonas genetics   isolation & purification   metabolism   MeSH
• Soil Microbiology * MeSH
• Blotting, Southern MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Czech Republic MeSH
• Names of Substances
• DNA, Bacterial MeSH
• Soil Pollutants MeSH
• Polychlorinated Biphenyls MeSH

Moderately halophilic bacteria utilizing phenol as the sole carbon source were isolated by selective enrichment from sea water. The isolate (Gram-negative motile rods) was identified as Deleya venusta. It grew well in the presence of up to 1600 mg/L of phenol and 8% NaCl under aerobic conditions. When the cells were treated with chloramphenicol prior to the addition of phenol they did not utilize added phenol, even after prolonged incubation. Thus, the enzymes necessary for phenol metabolism appeared to be inducible.

• MeSH
• Arthrobacter classification   growth & development   isolation & purification   MeSH
• Bacteria classification   growth & development   isolation & purification   MeSH
• Bacteriological Techniques MeSH
• Biodegradation, Environmental MeSH
• Water Pollutants, Chemical metabolism   MeSH
• Sodium Chloride MeSH
• Phenol metabolism   MeSH
• Halomonas classification   growth & development   isolation & purification   MeSH
• Culture Media MeSH
• Seawater microbiology   MeSH
• Vibrio classification   growth & development   isolation & purification   MeSH
• Publication type
• Journal Article MeSH
• Evaluation Study MeSH
• Names of Substances
• Water Pollutants, Chemical MeSH
• Sodium Chloride MeSH
• Phenol MeSH
• Culture Media MeSH

Two oil-degrading bacteria identified as Pseudomonas aeruginosa and Micrococcus luteus were isolated from crude-oil-polluted soils in Nigeria. The organisms were grown on n-hexadecane and sodium succinate and then examined for the presence of hydrocarbon inclusions. Inclusion bodies were found in n-hexadecane-grown cells and were absent in succinate-grown cells. Formation of hydrocarbon inclusion bodies appears to be a general phenomenon among hydrocarbon utilizers.

• MeSH
• Alkanes metabolism   MeSH
• Inclusion Bodies ultrastructure   MeSH
• Culture Media MeSH
• Soil Pollutants * MeSH
• Micrococcus luteus isolation & purification   metabolism   ultrastructure   MeSH
• Pseudomonas aeruginosa isolation & purification   metabolism   ultrastructure   MeSH
• Soil Microbiology * MeSH
• Petroleum metabolism   MeSH
• Succinates MeSH
• Tropical Climate MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Alkanes MeSH
• Culture Media MeSH
• Soil Pollutants * MeSH
• n-hexadecane MeSH Browser
• Petroleum MeSH
• Succinates MeSH

A strain of bacteria able to detoxicate the herbicide diuron in pure culture was isolated from sites contaminated with different urea herbicides. Diuron was used as a sole source of carbon and energy by this isolate which is a Gram-negative, aerobic, rod-shaped bacterium with a single polar flagellum, and grows at 40 degrees C. The strain has been identified as Pseudomonas sp.

• MeSH
• Biodegradation, Environmental MeSH
• Diuron metabolism   MeSH
• Herbicides metabolism   MeSH
• Culture Media MeSH
• Soil Pollutants metabolism   MeSH
• Pseudomonas growth & development   isolation & purification   metabolism   MeSH
• Soil Microbiology * MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Diuron MeSH
• Herbicides MeSH
• Culture Media MeSH
• Soil Pollutants MeSH

Spread-plating belongs to traditional microbiological methods employed for quantification of subsurface microflora during bioremediation projects in the Czechia. Concentration of degrading organisms is estimated from the number of colonies grown on agar plates supplied with contaminant as the sole carbon source. The data obtained during in situ bioremediation of the Dacice site contaminated by cutting oil suggests that changes in the composition of the carbon source in the subsurface may cause a discrepancy between laboratory data and situation in subsurface.

• MeSH
• Bacteria isolation & purification   metabolism   MeSH
• Bacteriological Techniques * MeSH
• Biodegradation, Environmental MeSH
• Culture Media MeSH
• Environmental Microbiology * MeSH
• Oils metabolism   MeSH
• Carbon MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Culture Media MeSH
• Oils MeSH
• Carbon MeSH