The red yeast Rhodotorula mucilaginosa strain CRUB 0138 (previously identified as R. lactosa) was isolated from a high-altitude Patagonian Lake Toncek (1700 m a.s.l.), and assigned with mucilaginosa species. Its biochemical, physiological and molecular features were assessed and compared to R. mucilaginosa PYCC 5166 type strain using a polyphasic approach; in addition, biomass and carotenoid pigment production at different C/N ratios were determined in an incubator shaker. Phenetic characterization by means of 70 current physiological tests including assimilation of aldaric acids and aromatic compounds, and also the ability to grow with amino acids as sole carbon sources, was carried out. According to numerical taxonomy calculations, similarity indexes between R. mucilaginosa CRUB 0138 and PYCC 5166 type strain were 0.86 and 0.77, corresponding to a complete set of physiological tests and MSP-PCR (Mini/Micro Satellite Primed PCR; (GTG)5, M13 and (GAC)5 primers were employed) fingerprinting. Killer activity against 2 native strains, Rhodosporidium kratochvilovae and R. mucilaginosa was detected. Maximum biomass-glucose conversion efficiency (87%) and maximum carotenoid yield (2.32 mg/L) were obtained at C/N = 5 in culture medium containing 10 and 40 g/L glucose, respectively. Different C/N ratios did not influence carotenoid pigment production but low C/N enhanced biomass yield.

• MeSH
• antibióza MeSH
• Basidiomycota růst a vývoj   MeSH
• biologické pigmenty biosyntéza   MeSH
• biomasa MeSH
• DNA fingerprinting MeSH
• DNA fungální analýza   izolace a purifikace   MeSH
• dusík metabolismus   MeSH
• glukosa metabolismus   MeSH
• karotenoidy biosyntéza   MeSH
• mikrobiologie vody * MeSH
• mikrosatelitní repetice genetika   MeSH
• mykologické určovací techniky MeSH
• polymerázová řetězová reakce MeSH
• Rhodotorula klasifikace   izolace a purifikace   metabolismus   fyziologie   MeSH
• sladká voda mikrobiologie   MeSH
• uhlík metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Geografické názvy
• Argentina MeSH
• Názvy látek
• biologické pigmenty MeSH
• DNA fungální MeSH
• dusík MeSH
• glukosa MeSH
• karotenoidy MeSH
• uhlík MeSH

Eighty-one strains of viable microorganisms were recovered from 23 samples collected from Ice Core 3 of Malan Glacier (China, 91 degrees 45.3' E, 35 degrees 48.4' N) drilled at high altitude (5620 m). All the strains were prokaryotes--75 of bacteria (including spore-forming ones) and 6 of actinomycetes. The characteristic genera differ from those of Arctic and Antarctic ice, in which many fungi and algae are widely distributed; this shows an difference of environmental conditions between Tibet and polar regions. The variation in number and species of Bacillus in different ice core layers implied changes of environmental conditions in the past.

• MeSH
• Bacteria chemie   klasifikace   izolace a purifikace   MeSH
• Eukaryota klasifikace   izolace a purifikace   MeSH
• houby klasifikace   izolace a purifikace   MeSH
• led MeSH
• mikrobiologie vody * MeSH
• studené klima * MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Tibet MeSH
• Názvy látek
• led MeSH

Yeast strains Cryptococcus albidus, Cryptococcus laurentii, Rhodotorula minuta were isolated from a moss sample. Candida oleophila and Rhodotorula mucilaginosa were isolated from a soil sample taken from Livingston Island. Antarctica. Fatty acid, phospholipid, sterol and tocopherol composition was determined in separated lipid fraction after fermentation in a medium containing glucose, peptone and yeast extract. Unsaturated fatty acids, mainly oleic (51-65%) and linoleic (9.5-16.8%), predominated in triacylglycerols. Sterols represent ca. 120-930 mg per kg dry biomass. The content of major phospholipids (phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine) was ca. 100-800 mg/kg. The amount of tocopherols (mainly gamma- and delta-tocopherol) was 2.1-6.3 mg/kg.

• MeSH
• fosfolipidy analýza   MeSH
• kvasinky chemie   MeSH
• lipidy analýza   chemie   MeSH
• mastné kyseliny analýza   MeSH
• půdní mikrobiologie MeSH
• steroly analýza   MeSH
• tokoferoly analýza   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Antarktida MeSH
• Názvy látek
• fosfolipidy MeSH
• lipidy MeSH
• mastné kyseliny MeSH
• steroly MeSH
• tokoferoly MeSH