Primers were designed and prepared and conditions were determined for PCR detection and differentiation of enterotoxigenic E. coli bacterial strains isolated from diarrheic pigs. Primers K88/1 and K88/2 are 25 bp oligomers that correspond to a region of genes encoding one of serological variants of the K88 antigen (K88ab(1), K88ab(2), K88ac or K88ad). A positive result of PCR is an amplificate of 792 bp in size for K88ab and K88ad variant or 786 bp for K88ac variant. The individual serological variants of genes of the K88 antigen could be differentiated by cutting the obtained PCR amplificates by restriction endonucleases. The PCR analysis of 674 E. coli strains isolated from diarrheic pigs showed that 184 strains were K88 positive. By using restriction endonucleases the K88-positive strains were in 4 cases classified as K88ab variant, 180 as K88ac variant and none contained gene for the K88ad variant. Ninety-five % coincidence with serological examination using K88ab, K88ac and K88ad specific antibodies was shown.

• MeSH
• antigeny bakteriální analýza   klasifikace   genetika   MeSH
• DNA primery MeSH
• Escherichia coli klasifikace   genetika   izolace a purifikace   MeSH
• genetická variace MeSH
• infekce vyvolané Escherichia coli mikrobiologie   veterinární   MeSH
• nemoci prasat mikrobiologie   MeSH
• polymerázová řetězová reakce metody   MeSH
• prasata MeSH
• proteiny fimbrií analýza   klasifikace   genetika   MeSH
• proteiny z Escherichia coli analýza   klasifikace   genetika   MeSH
• průjem mikrobiologie   veterinární   MeSH
• restrikční enzymy metabolismus   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Slovenská republika MeSH
• Názvy látek
• antigeny bakteriální MeSH
• DNA primery MeSH
• K88 antigen, E coli MeSH Prohlížeč
• proteiny fimbrií MeSH
• proteiny z Escherichia coli MeSH
• restrikční enzymy MeSH

Exoproteinase production was demonstrated in 64 clinical isolates of S. marcescens. A significant relationship was found between the site of origin (autopsy material, hemocultures, various other sources), proteinase activity, and LD50 of the analyzed isolates. The number of exoproteinases varied during a 14-h incubation in batch cultures; the most frequently found was a 57.5-kDa proteinase which was observed in all analyzed strains. The exoproteinase production was shown to be related to strain virulence.

• MeSH
• exopeptidasy metabolismus   MeSH
• lidé MeSH
• Serratia marcescens enzymologie   patogenita   fyziologie   MeSH
• virulence MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• exopeptidasy MeSH

A total of 5 Bifidobacterium spp. isolated from pig and children' feces and 6 Lactobacillus spp. from chicken feces were examined for expression of aggregation, cell surface hydrophobicity (CSH) and adherence to intestinal mucin. Co-aggregation activity was seen in 3 strains of auto-aggregative bifidobacteria and 4 auto-aggregative strains of Lactobacillus spp. with 2 enterohemorrhagic E. coli (O157). CSH correlated with Lactobacillus auto-aggregating activity and adherence to mucin but the correlation between Bifidobacterium adherence to mucin and CSH was not confirmed.

• MeSH
• bakteriální adheze * MeSH
• Bifidobacterium izolace a purifikace   fyziologie   MeSH
• Escherichia coli O157 fyziologie   MeSH
• feces mikrobiologie   MeSH
• hydrofobní a hydrofilní interakce MeSH
• kur domácí mikrobiologie   MeSH
• Lactobacillus izolace a purifikace   fyziologie   MeSH
• lidé MeSH
• muciny metabolismus   MeSH
• prasata mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• muciny MeSH

To determine the association of enteroaggregative (EAEC) and cell-detaching (CDEC) Escherichia coli with diarrhea of unknown origin among children from Wrocław (Poland), E. coli strains isolated from stool specimens of children with diarrhea were examined for mannose-resistant adherence to HEp-2 cells. EAEC were isolated from 10 of 39 (26%) children examined with diarrhea and 4 of 20 (20%) age-matched controls. CDEC were present in 14 (36%) cases of diarrhea and 7 (35%) healthy subjects. Cell-detaching activity was distinctly associated with hemolysin production. Among hemolytic CDEC strains cytotoxic necrotizing factor 1 (CNF1) synthesis prevailed among isolates obtained from cases of diarrhea (57%) in comparison with isolates obtained from healthy controls (14.3%). Although neither EAEC nor CDEC E. coli strains were associated with diarrhea of children in this setting, there were differences among EAEC and CDEC strains isolated from children with and without diarrhea.

• MeSH
• bakteriální adheze MeSH
• bakteriální toxiny metabolismus   MeSH
• buněčná adheze MeSH
• cytotoxiny metabolismus   MeSH
• Escherichia coli klasifikace   metabolismus   patogenita   MeSH
• feces mikrobiologie   MeSH
• HeLa buňky MeSH
• hemolyziny metabolismus   MeSH
• hepatocelulární karcinom MeSH
• infekce vyvolané Escherichia coli diagnóza   mikrobiologie   MeSH
• kojenec MeSH
• lidé MeSH
• proteiny z Escherichia coli * MeSH
• průjem diagnóza   mikrobiologie   MeSH
• virulence MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Polsko MeSH
• Názvy látek
• bakteriální toxiny MeSH
• cytotoxic necrotizing factor type 1 MeSH Prohlížeč
• cytotoxiny MeSH
• hemolyziny MeSH
• proteiny z Escherichia coli * MeSH

The prevalence of extended-spectrum beta-lactamases (ESBL) was determined among isolates of Escherichia coli (n = 63) isolated from hospitalized (43) and healthy (20) children. Ten isolates (21%) were ESBL-positive for two screening tests, the double disk-synergy test and the Oxoid Combination Disk method. One ESBL-positive isolate came from a healthy child. The transfer frequency of oxyimino-beta-lactam resistance from ESBL-producing isolates to E. coli K12 C600 recipient strain ranged from 10(-8) to 10(-5) per donor cell. Donor strains and transconjugants displayed susceptibility patterns typical of ESBL producers. They were resistant to oxyimino-beta-lactams but susceptible to clavulanic acid and carbapenems. Seven out of the 10 ESBL-positive isolates were found to produce MR/MS fimbria, which may play an important role in the colonization of the human intestinal mucosa.

• MeSH
• beta-laktamasy metabolismus   MeSH
• beta-laktamová rezistence MeSH
• beta-laktamy farmakologie   MeSH
• dítě MeSH
• Escherichia coli klasifikace   enzymologie   izolace a purifikace   MeSH
• hospitalizace * MeSH
• infekce vyvolané Escherichia coli epidemiologie   mikrobiologie   MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• předškolní dítě MeSH
• průjem epidemiologie   mikrobiologie   MeSH
• střevní sliznice mikrobiologie   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• předškolní dítě MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• beta-laktamasy MeSH
• beta-laktamy MeSH

Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli virulence factors (shiga toxin 1 and 2, intimin and enterohemolysin) and their genes. Vero-cell assay and latex agglutination were used for detection of Shiga toxin 1 and 2, TSB agar with washed erythrocytes was used for detection of enterohemolysin; genes encoding shiga toxin 1 and 2, intimin and enterohemolysin were detected using multiplex PCR. The presence of E. coli strains harboring genes encoding shiga toxin 1 and 2 (12 strains), intimin (34 strains) and enterohemolysin (12 strains) was demonstrated.

• MeSH
• bakteriální adheziny analýza   MeSH
• dítě MeSH
• Escherichia coli chemie   izolace a purifikace   MeSH
• feces mikrobiologie   MeSH
• hemolyziny analýza   MeSH
• infekce vyvolané Escherichia coli mikrobiologie   MeSH
• lidé MeSH
• polymerázová řetězová reakce MeSH
• předškolní dítě MeSH
• proteiny z Escherichia coli * MeSH
• shiga toxiny analýza   MeSH
• transportní proteiny analýza   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• předškolní dítě MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Slovenská republika MeSH
• Názvy látek
• bakteriální adheziny MeSH
• eaeA protein, E coli MeSH Prohlížeč
• enterohemolysin MeSH Prohlížeč
• hemolyziny MeSH
• proteiny z Escherichia coli * MeSH
• shiga toxiny MeSH
• transportní proteiny MeSH

P-fimbriae, S-fimbriae and AFA-adhesins are virulence factors responsible for adherence of Escherichia coli strains to extraintestinal host-cell surface. Detection of pap-, sfa- and afa-specific sequences performed by PCR revealed 74% pap+, 65% sfa+, and 8.3% afa+ strains in a group of 84 extraintestinal E. coli isolates. Detection in a group of fecal strains showed 29% pap+, 21% sfa+ and 4% afa+ strains. pap together with sfa were found as the most frequent combination (56%) among extraintestinal isolates probably due to localization of pap- and sfa-operons on a common pathogenicity island. The occurrence of afa-specific sequence among 56 urine strains was 11%, although no afa+ strain was detected among 28 gynecological isolates. No strains with detected adhesin operons were found among twenty (24%) extraintestinal E. coli strains.

• MeSH
• adheziny Escherichia coli genetika   fyziologie   MeSH
• bakteriální adheze fyziologie   MeSH
• bakteriální fimbrie fyziologie   MeSH
• cystitida mikrobiologie   MeSH
• dítě MeSH
• DNA bakterií chemie   genetika   MeSH
• dospělí MeSH
• Escherichia coli genetika   izolace a purifikace   patogenita   MeSH
• gynekologická onemocnění mikrobiologie   MeSH
• infekce vyvolané Escherichia coli mikrobiologie   MeSH
• kojenec MeSH
• lidé středního věku MeSH
• lidé MeSH
• polymerázová řetězová reakce MeSH
• předškolní dítě MeSH
• pyelonefritida mikrobiologie   MeSH
• virulence MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• lidé středního věku MeSH
• lidé MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• adheziny Escherichia coli MeSH
• DNA bakterií MeSH