BACKGROUND: The role of fatty acids (FA) in the pathogenesis of insulin resistance and hyperlipidemia is a subject of intensive research. Several recent works have suggested cis-vaccenic acid (cVA) in plasma lipid compartments, especially in plasma phospholipids (PL) or erythrocyte membranes, could be associated with markers of insulin sensitivity and cardiovascular health. Nevertheless, not all the results of research work testify to these beneficial effects of cVA. Therefore, we decided to investigate the relations of proportion of cVA in plasma PL to markers of insulin resistance in hyperlipidemic men. SUBJECTS: In 231 men (median age 50) with newly diagnosed hyperlipidemia, we analyzed basic clinical parameters together with FA composition of plasma PL and stratified them according to the content of cVA into upper quartile (Q4) and lower quartile (Q1) groups. We examined also small control group of 50 healthy men. RESULTS: The individuals in Q4 differed from Q1 by lower plasma insulin (p < 0.05), HOMA-IR values (p < 0.01), and apolipoprotein B concentrations (p < 0.001), but by the higher total level of nonesterified FA (p < 0.01). Both groups had similar age, anthropometrical, and other lipid parameters. In plasma PL, the Q4 group had lower content of the sum of n-6 polyunsaturated FA, due to decrease of γ-linolenic and dihomo-γ-linolenic acids, whereas the content of monounsaturated FA (mainly oleic and palmitoleic) was in Q4 higher. CONCLUSIONS: Our results support hypothesis that plasma PL cVA could be associated with insulin sensitivity in men with hyperlipidemia.

• MeSH
• Apolipoproteins B blood   MeSH
• Biomarkers * blood   MeSH
• Adult MeSH
• Phospholipids * blood   MeSH
• Hyperlipidemias * blood   MeSH
• Insulin blood   MeSH
• Insulin Resistance * MeSH
• Oleic Acids * blood   MeSH
• Middle Aged MeSH
• Humans MeSH
• Check Tag
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Male MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Apolipoproteins B MeSH
• Biomarkers * MeSH
• cis-vaccenic acid MeSH Browser
• Phospholipids * MeSH
• Insulin MeSH
• Oleic Acids * MeSH

OBJECTIVE: In the pathogenesis of the metabolic syndrome (MetS), an increase of oxidative stress could play an important role which is closely linked with insulin resistance, endothelial dysfunction, and chronic inflammation. The aim of our study was to assess several parameters of the antioxidant status in MetS. METHODS: 40 subjects with MetS and 40 age- and sex-matched volunteers without MetS were examined for activities of superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase 1 (GPX1), glutathione reductase (GR), paraoxonase1 (PON1), concentrations of reduced glutathione (GSH), and conjugated dienes in low-density lipoprotein (CD-LDL). RESULTS: Subjects with MetS had higher activities of CuZnSOD (p < 0.05) and GR (p < 0.001), higher concentrations of CD-LDL (p < 0.001), lower activities of CAT (p < 0.05) and PON1 (p < 0.05), and lower concentrations of GSH (p < 0.05), as compared with controls. Activity of GPX1 was not significantly changed. CONCLUSIONS: Our results implicated an increased oxidative stress in MetS and a decreased antioxidative defense that correlated with some laboratory (triglycerides, high-density lipoprotein cholesterol (HDL-C)) and clinical (waist circumference, blood pressure) components of MetS.

• MeSH
• Antioxidants analysis   MeSH
• Aryldialkylphosphatase blood   MeSH
• Biomarkers blood   MeSH
• Enzymes blood   MeSH
• Glutathione blood   MeSH
• Glutathione Peroxidase GPX1 MeSH
• Glutathione Peroxidase blood   MeSH
• Glutathione Reductase blood   MeSH
• Catalase blood   MeSH
• Middle Aged MeSH
• Humans MeSH
• Lipoproteins, LDL blood   MeSH
• Metabolic Syndrome blood   diagnosis   enzymology   MeSH
• Oxidative Stress MeSH
• Case-Control Studies MeSH
• Superoxide Dismutase blood   MeSH
• Check Tag
• Middle Aged MeSH
• Humans MeSH
• Male MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Antioxidants MeSH
• Aryldialkylphosphatase MeSH
• Biomarkers MeSH
• Enzymes MeSH
• Glutathione MeSH
• Glutathione Peroxidase GPX1 MeSH
• Glutathione Peroxidase MeSH
• Glutathione Reductase MeSH
• GPX1 protein, human MeSH Browser
• Catalase MeSH
• Lipoproteins, LDL MeSH
• PON1 protein, human MeSH Browser
• Superoxide Dismutase MeSH