Immune responses to intracellular pathogens depend largely upon the activation of T helper type 1-dependent mechanisms. The contribution of B cells to establishing protective immunity has long been underestimated. Francisella tularensis, including a number of subspecies, provides a suitable model for the study of immune responses against intracellular bacterial pathogens. We previously demonstrated that Francisella infects B cells and activates B-cell subtypes to produce a number of cytokines and express the activation markers. Recently, we documented the early production of natural antibodies as a consequence of Francisella infection in mice. Here, we summarize current knowledge on the innate and acquired humoral immune responses initiated by Francisella infection and their relationships with the immune defense systems.

• Klíčová slova
• B cells, Francisella tularensis, intracellular pathogen, natural antibodies, natural immunity,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

SARS-CoV-2 infection induces the production of autoantibodies, which is significantly associated with complications during hospitalization and a more severe prognosis in COVID-19 patients. Such a response of the patient's immune system may reflect (1) the dysregulation of the immune response or (2) it may be an attempt to regulate itself in situations where the non-infectious self poses a greater threat than the infectious non-self. Of significance may be the primary virus-host cell interaction where the surface-bound ACE2 ectoenzyme plays a critical role. Here, we present a brief analysis of recent findings concerning the immune recognition of SARS-CoV-2, which, we believe, favors the second possibility as the underlying reason for the production of autoantibodies during COVID-19.

• Klíčová slova
• ACE2 signaling, COVID-19, SARS-CoV-2, autoantibodies, innate immune recognition,
• Publikační typ
• časopisecké články MeSH

Immunoproteomic analysis was applied to study the immunoreactivity of serum samples collected at different time points from a laboratory assistant accidentally infected with highly virulent strain of Francisella tularensis subsp. tularensis. Immunoblotting showed that the spectrum of F. tularensis antigens recognized specifically by immune sera remained with the exception for 1 antigen stable for up to 16 years after infection. Using immunoproteomics approach 10 immunoreactive antigens were successfully identified. Several new immunogenic F. tularensis proteins were described for the first time.

• MeSH
• 2D gelová elektroforéza MeSH
• antigeny bakteriální imunologie   izolace a purifikace   MeSH
• bakteriální proteiny imunologie   izolace a purifikace   MeSH
• časové faktory MeSH
• Francisella tularensis imunologie   MeSH
• hmotnostní spektrometrie MeSH
• imunoblotting MeSH
• laboratorní infekce krev   MeSH
• lidé MeSH
• protilátky bakteriální krev   imunologie   MeSH
• specificita protilátek MeSH
• tularemie krev   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny bakteriální MeSH
• bakteriální proteiny MeSH
• protilátky bakteriální MeSH