We asked whether acute redox signaling from mitochondria exists concomitantly to fatty acid- (FA-) stimulated insulin secretion (FASIS) at low glucose by pancreatic β-cells. We show that FA β-oxidation produces superoxide/H2O2, providing: i) mitochondria-to-plasma-membrane redox signaling, closing KATP-channels synergically with elevated ATP (substituting NADPH-oxidase-4-mediated H2O2-signaling upon glucose-stimulated insulin secretion); ii) activation of redox-sensitive phospholipase iPLA2γ/PNPLA8, cleaving mitochondrial FAs, enabling metabotropic GPR40 receptors to amplify insulin secretion (IS). At fasting glucose, palmitic acid stimulated IS in wt mice; palmitic, stearic, lauric, oleic, linoleic, and hexanoic acids also in perifused pancreatic islets (PIs), with suppressed 1st phases in iPLA2γ/PNPLA8-knockout mice/PIs. Extracellular/cytosolic H2O2-monitoring indicated knockout-independent redox signals, blocked by mitochondrial antioxidant SkQ1, etomoxir, CPT1 silencing, and catalase overexpression, all inhibiting FASIS, keeping ATP-sensitive K+-channels open, and diminishing cytosolic [Ca2+]-oscillations. FASIS in mice was a postprandially delayed physiological event. Redox signals of FA β-oxidation are thus documented, reaching the plasma membrane, essentially co-stimulating IS.

• Klíčová slova
• Fatty acid-stimulated insulin secretion, GPR40, Mitochondrial fatty acids, Pancreatic β-cells, Redox signaling, Redox-activated phospholipase iPLA2γ,
• MeSH
• beta-buňky * metabolismus   MeSH
• buněčná membrána * metabolismus   MeSH
• fosfolipasy A2, skupina VI metabolismus   genetika   MeSH
• glukosa metabolismus   MeSH
• inzulin metabolismus   MeSH
• mastné kyseliny * metabolismus   MeSH
• mitochondrie * metabolismus   MeSH
• myši knockoutované MeSH
• myši MeSH
• oxidace-redukce * MeSH
• peroxid vodíku metabolismus   MeSH
• receptory spřažené s G-proteiny MeSH
• sekrece inzulinu * MeSH
• signální transdukce * MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• Ffar1 protein, mouse MeSH Prohlížeč
• fosfolipasy A2, skupina VI MeSH
• glukosa MeSH
• inzulin MeSH
• mastné kyseliny * MeSH
• peroxid vodíku MeSH
• Pla2g6 protein, mouse MeSH Prohlížeč
• receptory spřažené s G-proteiny MeSH

Mitochondria (mt) represent the vital hub of the molecular physiology of the cell, being decision-makers in cell life/death and information signaling, including major redox regulations and redox signaling. Now we review recent advances in understanding mitochondrial redox homeostasis, including superoxide sources and H2O2 consumers, i.e., antioxidant mechanisms, as well as exemplar situations of physiological redox signaling, including the intramitochondrial one and mt-to-cytosol redox signals, which may be classified as acute and long-term signals. This review exemplifies the acute redox signals in hypoxic cell adaptation and upon insulin secretion in pancreatic beta-cells. We also show how metabolic changes under these circumstances are linked to mitochondrial cristae narrowing at higher intensity of ATP synthesis. Also, we will discuss major redox buffers, namely the peroxiredoxin system, which may also promote redox signaling. We will point out that pathological thresholds exist, specific for each cell type, above which the superoxide sources exceed regular antioxidant capacity and the concomitant harmful processes of oxidative stress subsequently initiate etiology of numerous diseases. The redox signaling may be impaired when sunk in such excessive pro-oxidative state.

• MeSH
• antioxidancia metabolismus   MeSH
• beta-buňky metabolismus   MeSH
• lidé MeSH
• mitochondrie * metabolismus   MeSH
• oxidace-redukce * MeSH
• oxidační stres fyziologie   MeSH
• signální transdukce fyziologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• antioxidancia MeSH

Progress in mass spectroscopy of posttranslational oxidative modifications has enabled researchers to experimentally verify the concept of redox signaling. We focus here on redox signaling originating from mitochondria under physiological situations, discussing mechanisms of transient redox burst in mitochondria, as well as the possible ways to transfer such redox signals to specific extramitochondrial targets. A role of peroxiredoxins is described which enables redox relay to other targets. Examples of mitochondrial redox signaling are discussed: initiation of hypoxia-inducible factor (HIF) responses; retrograde redox signaling to PGC1α during exercise in skeletal muscle; redox signaling in innate immune cells; redox stimulation of insulin secretion, and other physiological situations.

• Klíčová slova
• H2O2 diffusion, HIF, Redox signaling from mitochondria, mitochondrial superoxide formation, peroxiredoxins, redox-regulation of kinases,
• MeSH
• beta-buňky metabolismus   MeSH
• hypoxie metabolismus   MeSH
• imunita fyziologie   MeSH
• kosterní svaly metabolismus   MeSH
• mitochondrie metabolismus   MeSH
• oxidace-redukce MeSH
• peroxid vodíku metabolismus   MeSH
• peroxiredoxiny MeSH
• posttranslační úpravy proteinů MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• signální transdukce fyziologie   MeSH
• superoxidy metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• peroxid vodíku MeSH
• peroxiredoxiny MeSH
• reaktivní formy kyslíku MeSH
• superoxidy MeSH

Significance: Type 2 diabetes development involves multiple changes in β-cells, related to the oxidative stress and impaired redox signaling, beginning frequently by sustained overfeeding due to the resulting lipotoxicity and glucotoxicity. Uncovering relationships among the dysregulated metabolism, impaired β-cell "well-being," biogenesis, or cross talk with peripheral insulin resistance is required for elucidation of type 2 diabetes etiology. Recent Advances: It has been recognized that the oxidative stress, lipotoxicity, and glucotoxicity cannot be separated from numerous other cell pathology events, such as the attempted compensation of β-cell for the increased insulin demand and dynamics of β-cell biogenesis and its "reversal" at dedifferentiation, that is, from the concomitantly decreasing islet β-cell mass (also due to transdifferentiation) and low-grade islet or systemic inflammation. Critical Issues: At prediabetes, the compensation responses of β-cells, attempting to delay the pathology progression-when exaggerated-set a new state, in which a self-checking redox signaling related to the expression of Ins gene expression is impaired. The resulting altered redox signaling, diminished insulin secretion responses to various secretagogues including glucose, may lead to excretion of cytokines or chemokines by β-cells or excretion of endosomes. They could substantiate putative stress signals to the periphery. Subsequent changes and lasting glucolipotoxicity promote islet inflammatory responses and further pathology spiral. Future Directions: Should bring an understanding of the β-cell self-checking and related redox signaling, including the putative stress signal to periphery. Strategies to cure or prevent type 2 diabetes could be based on the substitution of the "wrong" signal by the "correct" self-checking signal.

• Klíčová slova
• dedifferentiation, impaired redox signaling, oxidative stress, pancreatic β-cells, type 2 diabetes, β-cell identity self-checking,
• MeSH
• beta-buňky metabolismus   MeSH
• diabetes mellitus 2. typu metabolismus   MeSH
• lidé MeSH
• oxidační stres genetika   fyziologie   MeSH
• signální transdukce MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

AIMS: Pancreatic β-cell chronic lipotoxicity evolves from acute free fatty acid (FA)-mediated oxidative stress, unprotected by antioxidant mechanisms. Since mitochondrial uncoupling protein-2 (UCP2) plays antioxidant and insulin-regulating roles in pancreatic β-cells, we tested our hypothesis, that UCP2-mediated uncoupling attenuating mitochondrial superoxide production is initiated by FA release due to a direct H2O2-induced activation of mitochondrial phospholipase iPLA2γ. RESULTS: Pro-oxidant tert-butylhydroperoxide increased respiration, decreased membrane potential and mitochondrial matrix superoxide release rates of control but not UCP2- or iPLA2γ-silenced INS-1E cells. iPLA2γ/UCP2-mediated uncoupling was alternatively activated by an H2O2 burst, resulting from palmitic acid (PA) β-oxidation, and it was prevented by antioxidants or catalase overexpression. Exclusively, nascent FAs that cleaved off phospholipids by iPLA2γ were capable of activating UCP2, indicating that the previously reported direct redox UCP2 activation is actually indirect. Glucose-stimulated insulin release was not affected by UCP2 or iPLA2γ silencing, unless pro-oxidant activation had taken place. PA augmented insulin secretion via G-protein-coupled receptor 40 (GPR40), stimulated by iPLA2γ-cleaved FAs (absent after GPR40 silencing). INNOVATION AND CONCLUSION: The iPLA2γ/UCP2 synergy provides a feedback antioxidant mechanism preventing oxidative stress by physiological FA intake in pancreatic β-cells, regulating glucose-, FA-, and redox-stimulated insulin secretion. iPLA2γ is regulated by exogenous FA via β-oxidation causing H2O2 signaling, while FAs are cleaved off phospholipids, subsequently acting as amplifying messengers for GPR40. Hence, iPLA2γ acts in eminent physiological redox signaling, the impairment of which results in the lack of antilipotoxic defense and contributes to chronic lipotoxicity.

• MeSH
• antioxidancia farmakologie   MeSH
• beta-buňky účinky léků   MeSH
• fosfolipasy A2, skupina II metabolismus   MeSH
• inzulin metabolismus   MeSH
• iontové kanály metabolismus   MeSH
• krysa rodu Rattus MeSH
• lipidy toxicita   MeSH
• membránový potenciál mitochondrií účinky léků   MeSH
• mitochondriální proteiny metabolismus   MeSH
• mitochondrie účinky léků   MeSH
• nádorové buněčné linie MeSH
• oxidační stres účinky léků   MeSH
• peroxid vodíku metabolismus   MeSH
• receptory spřažené s G-proteiny metabolismus   MeSH
• sekrece inzulinu MeSH
• signální transdukce účinky léků   MeSH
• superoxidy metabolismus   MeSH
• terc-butylhydroperoxid farmakologie   MeSH
• uncoupling protein 2 MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• fosfolipasy A2, skupina II MeSH
• G-protein-coupled receptor 40, rat MeSH Prohlížeč
• inzulin MeSH
• iontové kanály MeSH
• lipidy MeSH
• mitochondriální proteiny MeSH
• peroxid vodíku MeSH
• receptory spřažené s G-proteiny MeSH
• superoxidy MeSH
• terc-butylhydroperoxid MeSH
• Ucp2 protein, rat MeSH Prohlížeč
• uncoupling protein 2 MeSH