Nejvíce citovaný článek - PubMed ID 13598900
Stimulation of chlortetracycline production by benzyl thiocyanate
Cell protein profiles of submerged cultures of Streptomyces aureofaciens cultivated in the absence or presence of 12 microM benzyl thiocyanate (BT) were analyzed by one-dimensional SDS polyacrylamide gel electrophoresis. Substantial increase in the intensity of the 13, 35, 37, 60, and 100 kDa protein bands was observed in cultures treated with BT. Similar increase in the 35, 37, and 60 kDa bands was found in a mutant blocked in the last chlortetracycline biosynthesis step. Effect of BT on the solid medium-grown cultures was also observed, with a more intensive substrate mycelium pigmentation and alteration in the spore size and shape as the most characteristic features. Earlier studies of BT effect involving those on the stimulation of chlortetracycline biosynthesis are summarized and a possible signal-transducing mechanism is discussed from the point of view of adaptation of S. aureofaciens to the uncoupling of oxidative phosphorylation.
- MeSH
- bakteriální proteiny metabolismus MeSH
- elektronová mikroskopie MeSH
- Streptomyces aureofaciens účinky léků metabolismus ultrastruktura MeSH
- tetracykliny biosyntéza MeSH
- thiokyanatany farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bakteriální proteiny MeSH
- benzyl thiocyanate MeSH Prohlížeč
- tetracykliny MeSH
- thiokyanatany MeSH
The localization of anhydrotetracycline oxygenase and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) was studied by determining the enzyme activities in subcellular fractions obtained by differential centrifugation of the mycelia of Streptomyces aureofaciens after lysozyme treatment. Glucose-6-phosphate dehydrogenase was a typical cytoplasmic enzyme both in the low- and high-production strain. Anhydrotetracycline oxygenase was found in the membrane fraction of the low-production strain. In the high-production strain, it was detected in several fractions, the highest activity being found in cytoplasm. The presence of 10 microM benzyl thiocyanate in the culture medium significantly changed the distribution of the latter enzyme in both strains. The redistribution of the enzymes is discussed with respect to tetracycline over-production.
- MeSH
- frakcionace buněk metody MeSH
- glukosa-6-fosfátdehydrogenasa metabolismus MeSH
- hořčík farmakologie MeSH
- kinetika MeSH
- oxygenasy metabolismus MeSH
- Streptomyces aureofaciens účinky léků enzymologie MeSH
- thiokyanatany farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- anhydrotetracycline oxygenase MeSH Prohlížeč
- benzyl thiocyanate MeSH Prohlížeč
- glukosa-6-fosfátdehydrogenasa MeSH
- hořčík MeSH
- oxygenasy MeSH
- thiokyanatany MeSH
The possibility of shortening the thermal sterilization time for cultivating media was demonstrated in chlortetracycline fermentation with an industrial strain of Streptomyces aureofaciens. The medium was artificially contaminated with a mixture of eight strains of G+ and G- bacteria isolated from contaminated industrial fermentors, and the following chemical agents, either alone or in combination, were added: formaldehyde, phenol. dimethylformamide, p-aminosalicylic acid and nitrofurazone. Dimethylformamide was inhibitory even at 0.08%. formaldehyde concentrations higher than 0.05%, Nitrofurazone stimulated chlortetracycline production. The best combination was 0.01% formaldehyde added before, and 2.10-3% nitrofurazone added after short sterilization at 120 degrees C.
- MeSH
- antibakteriální látky farmakologie MeSH
- Bacteria účinky léků MeSH
- chlortetracyklin biosyntéza MeSH
- dimethylformamid farmakologie MeSH
- druhová specificita MeSH
- fenoly farmakologie MeSH
- fermentace MeSH
- formaldehyd farmakologie MeSH
- kultivační média * MeSH
- kyselina aminosalicylová farmakologie MeSH
- nitrofurazon farmakologie MeSH
- sterilizace * MeSH
- Streptomyces aureofaciens metabolismus MeSH
- vysoká teplota MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- antibakteriální látky MeSH
- chlortetracyklin MeSH
- dimethylformamid MeSH
- fenoly MeSH
- formaldehyd MeSH
- kultivační média * MeSH
- kyselina aminosalicylová MeSH
- nitrofurazon MeSH
- MeSH
- bakteriologické techniky MeSH
- chlorid sodný farmakologie MeSH
- chlortetracyklin analýza biosyntéza MeSH
- kultivační média MeSH
- Streptomyces klasifikace metabolismus účinky záření MeSH
- tetracyklin analýza biosyntéza MeSH
- thiokyanatany farmakologie MeSH
- ultrafialové záření MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chlorid sodný MeSH
- chlortetracyklin MeSH
- kultivační média MeSH
- tetracyklin MeSH
- thiokyanatany MeSH
