The osmotolerant and cell wall properties of the two most studied wild-type Zygosaccharomyces rouxii strains (CBS 732 and ATCC 42981) were examined. Differences in their (1) tolerance to high salt content in the medium, (2) resistance to the lysing enzymes Lyticase and Zymolyase, (3) cell-wall polymer content and (4) cell wall micromorphology suggested that the less osmotolerant CBS 732 strain possesses a more rigid cell wall than the more osmotolerant ATCC 42981, whose cell wall seems to be more flexible and elastic.

• MeSH
• antifungální látky farmakologie   MeSH
• buněčná stěna fyziologie   ultrastruktura   MeSH
• endo-1,3-beta-glukanasa farmakologie   MeSH
• fyziologická adaptace MeSH
• hypertonický solný roztok metabolismus   MeSH
• multienzymové komplexy farmakologie   MeSH
• osmolární koncentrace MeSH
• proteasy farmakologie   MeSH
• Saccharomyces cerevisiae fyziologie   MeSH
• Zygosaccharomyces cytologie   fyziologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antifungální látky MeSH
• endo-1,3-beta-glukanasa MeSH
• hypertonický solný roztok MeSH
• lyticase MeSH Prohlížeč
• multienzymové komplexy MeSH
• proteasy MeSH

Morphological, developmental and antimetabolite-resistant mutants of T. lanuginosus were characterized and used for screening with the aim to develop constitutive alpha-amylase-hyperproducing strains. The protoplast fusion of two spontaneous mutants of T. lanuginosus, characterized as asporulating and resistant to 2-deoxy-D-glucose (2DG), resulted in sporulating, 2DG sensitive heterokaryotic fusants. A recombinant haploid strain F64fB developed there from produced alpha-amylase constitutively in glucose-containing medium. Constitutive alpha-amylase-hyperproducing mutant (III8) obtained after cyclic mutagenesis and screening yielded approximately 20 fold more alpha-amylase in a glycerol-containing medium than the wild strain.

• MeSH
• alfa-amylasy biosyntéza   MeSH
• Ascomycota enzymologie   genetika   růst a vývoj   MeSH
• biotechnologie metody   MeSH
• haploidie * MeSH
• morfogeneze MeSH
• mutace * MeSH
• mutageneze MeSH
• rekombinace genetická * MeSH
• spory hub MeSH
• vysoká teplota MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• alfa-amylasy MeSH