Potential virulence factors (elastase, proteinase, lipase, phospholipase C, alginate) as well as surface properties (hydrophobicity, motility) were determined in 103 Pseudomonas aeruginosa strains isolated from patients with cancer. Nontypable strains were the dominant group (60%), followed by serotypes O11 (17%), O12 (7%) and O4 (5%). Seventy-one strains (69%) produced high level of elastase (10-60 mg/L), 87% of the strains possessed high activity of proteinase (bacterial) (10-250 mg/L) and 69% of the strains demonstrated higher level of lipase (20-150 U/mL); these elevated levels of enzymes were associated mainly with nontypable strains. On the other hand, 79% of the strains did not produce or produced only a low level of phospholipase C and 60% of isolates did not manifest any or very low production of alginate. Hydrophobicity demonstrated by adherence of the bacteria to xylene was shown by 69% of strains; 94% of strains aggregated with ammonium sulfate. Motility in the range of 31-80 mm was found in 76 strains (74%). The considerable virulence of tested P. aeruginosa strains was confirmed. The nontypable strains manifested the most frequent group with high level of elastase, proteinase, lipase, hydrophobicity and motility.

• MeSH
• algináty metabolismus   MeSH
• bakteriální adheze MeSH
• endopeptidasy biosyntéza   MeSH
• fosfolipasy typu C biosyntéza   MeSH
• kyselina glukuronová MeSH
• kyseliny hexuronové MeSH
• lidé MeSH
• lipasa biosyntéza   MeSH
• nádory komplikace   mikrobiologie   MeSH
• oportunní infekce komplikace   mikrobiologie   MeSH
• pankreatická elastasa biosyntéza   MeSH
• pseudomonádové infekce komplikace   mikrobiologie   MeSH
• Pseudomonas aeruginosa klasifikace   izolace a purifikace   patogenita   fyziologie   MeSH
• sérotypizace MeSH
• virulence MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• algináty MeSH
• endopeptidasy MeSH
• fosfolipasy typu C MeSH
• kyselina glukuronová MeSH
• kyseliny hexuronové MeSH
• lipasa MeSH
• pankreatická elastasa MeSH

Thirty-three uropathogenic strains of Pseudomonas aeruginosa were investigated for hemolytic activity in both bacterial broth culture filtrates and isolate lyzates, resistance to bactericidal activity of fresh human serum, resistance to six antibiotics and plasmid DNA profile. Twenty-four of the 33 (73%) bacterial filtrates showed lysis of rabbit erythrocytes, as did the three after guinea-pig erythrocyte treatment. Twelve of 33 isolate lysates showed in parallel lysis of both types of erythrocytes used. Serum resistance was found in 17 (52%) isolates, intermediate resistance in 15 (45 %) isolates and only one isolate showed serum sensitivity. Resistance to antibiotics was detected as follows (in %): tetracycline 94, kanamycin 79, chloramphenicol 76, septrin 73, ampicillin 64, streptomycin 45, gentamicin 18. None of the isolates investigated showed resistance to colistine. With the exception of one isolate, plasmid DNA was detected in all P. aeruginosa strains.

• MeSH
• antibiotická rezistence MeSH
• baktericidní aktivita krve MeSH
• dítě MeSH
• dospělí MeSH
• hemolýza MeSH
• hemolyziny biosyntéza   MeSH
• infekce močového ústrojí mikrobiologie   MeSH
• králíci MeSH
• lidé MeSH
• morčata MeSH
• plazmidy genetika   izolace a purifikace   MeSH
• předškolní dítě MeSH
• pseudomonádové infekce mikrobiologie   MeSH
• Pseudomonas aeruginosa genetika   izolace a purifikace   patogenita   MeSH
• techniky in vitro MeSH
• virulence MeSH
• zvířata MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• králíci MeSH
• lidé MeSH
• morčata MeSH
• předškolní dítě MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• hemolyziny MeSH

Aminoglycoside antibiotics, most effective at the level of 1/4 of the MIC, suppressed all the tested activities of P. aeruginosa, except cytotoxicity. Proteinase activity was decreased to 60% (gentamicin) and 63% (streptomycin), permeability was reduced to 61% (gentamicin) and 73% (streptomycin), phospholipase C to 13% (gentamicin) and 51% (streptomycin) of the control values. Subinhibitory concentrations of beta-lactams inhibited only phospholipase C activity to 89% (ticarcillin) and 64% (cefotaxim) of the control values. These antibiotics did not suppress the cytotoxic activity and increased protease activity up to 155% (ticarcillin) and 192% (cefotaxim) as well as permeability up to 121% (ticarcillin) and 154% (cefotaxim) of the control values.

• MeSH
• antibakteriální látky farmakologie   MeSH
• cefotaxim farmakologie   MeSH
• endopeptidasy účinky léků   MeSH
• fosfolipasy typu C metabolismus   MeSH
• gentamiciny farmakologie   MeSH
• mikrobiální testy citlivosti MeSH
• permeabilita buněčné membrány MeSH
• Pseudomonas aeruginosa účinky léků   metabolismus   MeSH
• streptomycin farmakologie   MeSH
• tikarcilin farmakologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• cefotaxim MeSH
• endopeptidasy MeSH
• fosfolipasy typu C MeSH
• gentamiciny MeSH
• streptomycin MeSH
• tikarcilin MeSH