Massive toxic blooms of cyanobacteria represent a major threat to water supplies worldwide. Here, the biological activities of lipopolysaccharide (LPS) isolated from Microcystis aeruginosa, the most prominent cyanobacteria in water bloom, were studied. LPS was isolated from complex environmental water bloom samples dominated by M. aeruginosa, and from laboratory cultures of non-axenic as well as axenic M. aeruginosa strains PCC7806 and HAMBI/UHCC130. Employing human blood-based in vitro tests, the LPS isolated from complex water bloom revealed the priming of both major blood phagocyte population monocytes and polymorphonuclear leukocytes documented by the increased surface expression of CD11b and CD66b. This was accompanied by a water bloom LPS-mediated dose-dependent induction of tumor necrosis factor α, interleukin-1β, and interleukin-6 production. In accordance with its priming effects, water bloom LPS induced significant activation of p38 and ERK1/2 kinases, as well as NF-κB phosphorylation, in isolated polymorphonuclear leukocytes. Interestingly, the pro-inflammatory potential of LPS from the axenic strain of M. aeruginosa was not lower compared to that of LPS isolated from non-axenic strains. In contrast to the biological activity, water bloom LPS revealed almost twice higher pyrogenicity levels compared to Escherichia coli LPS, as analyzed by the PyroGene test. Moreover, LPS from the non-axenic culture exhibited higher endotoxin activity in comparison to LPS from axenic strains. Taking the above findings together, M. aeruginosa LPS can contribute to the health risks associated with contamination by complex water bloom mass.

• Klíčová slova
• cyanobacteria, endotoxin, inflammation, leukocytes, lipopolysaccharide, water bloom,
• MeSH
• antigeny CD11b metabolismus   MeSH
• CD antigeny metabolismus   MeSH
• cytokiny krev   MeSH
• eutrofizace MeSH
• GPI-vázané proteiny metabolismus   MeSH
• kultivované buňky MeSH
• laboratoře MeSH
• leukocyty mononukleární účinky léků   metabolismus   MeSH
• lidé MeSH
• lipopolysacharidy toxicita   MeSH
• Microcystis * MeSH
• molekuly buněčné adheze metabolismus   MeSH
• přirozená imunita účinky léků   MeSH
• pyrogeny toxicita   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny CD11b MeSH
• CD antigeny MeSH
• CEACAM8 protein, human MeSH Prohlížeč
• cytokiny MeSH
• GPI-vázané proteiny MeSH
• ITGAM protein, human MeSH Prohlížeč
• lipopolysacharidy MeSH
• molekuly buněčné adheze MeSH
• pyrogeny MeSH

The regulatory functions of glycosaminoglycan hyaluronan (HA) are suggested to be dependent on its molecular weight (MW). Proinflammatory and stimulatory effects are proposed mainly for the low MW HA. However, the complex response of blood phagocytes to HA of different MW is unclear. Herein, the effects of highly purified HA of precisely defined MW (52, 250, and 970 kDa) on human blood phagocytes were tested. All MW HA activated blood phagocytes, including the spontaneous production of ROS, degranulation, and the production of tumor necrosis factor alpha, with low MW HA 52 kDa having the highest potency and high MW HA 970 kDa having the lowest potency. Interestingly, HA inhibited ROS production stimulated by opsonized zymosan particles and, in contrast, potentiated starch-activated ROS production, mostly independent of MW. Data showed a significant effect of HA of different MW on blood phagocytes, including high MW HA.

• MeSH
• antigeny CD11b krev   MeSH
• CD antigeny krev   MeSH
• degranulace buněk účinky léků   MeSH
• fagocyty účinky léků   fyziologie   MeSH
• GPI-vázané proteiny krev   MeSH
• kyselina hyaluronová chemie   farmakologie   MeSH
• lidé MeSH
• molekulová hmotnost MeSH
• molekuly buněčné adheze krev   MeSH
• reaktivní formy kyslíku krev   MeSH
• receptory komplementu 3b krev   MeSH
• techniky in vitro MeSH
• TNF-alfa krev   MeSH
• zánět krev   etiologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny CD11b MeSH
• CD antigeny MeSH
• CEACAM8 protein, human MeSH Prohlížeč
• CR1 protein, human MeSH Prohlížeč
• GPI-vázané proteiny MeSH
• ITGAM protein, human MeSH Prohlížeč
• kyselina hyaluronová MeSH
• molekuly buněčné adheze MeSH
• reaktivní formy kyslíku MeSH
• receptory komplementu 3b MeSH
• TNF-alfa MeSH

Hyiodine (high molecular weight hyaluronan combined with KI3 complex) is a new non-adhesive wound dressing which significantly improves the healing process. The aim of the study was to investigate the effects of Hyoidine on functional properties of isolated human keratinocytes and leukocytes, and on those of U937 and HL60 cell lines. While KI3 complex inhibited the viability and proliferation of the cells tested, Hyiodine did not have any significant effect. The expression of CD11b, CD62L and CD69 on PMNL, monocytes and lymphocytes, as well as the oxidative burst of blood neutrophils, were not changed. On the contrary, Hyiodine inhibited the PMA-activated oxidative burst and significantly increased the production of IL-6 and TNF-alpha by lymphocytes. It was concluded that hyaluronan content of Hyiodine reduces the toxic effect of KI3 complex on cells and speeds up the wound healing process by increasing the production of inflammatory cytokines.

• MeSH
• hemostatika farmakologie   MeSH
• HL-60 buňky MeSH
• imunitní systém cytologie   účinky léků   MeSH
• jod farmakologie   MeSH
• jodidy farmakologie   MeSH
• keratinocyty účinky léků   MeSH
• kyselina hyaluronová farmakologie   MeSH
• lidé MeSH
• U937 buňky MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• hemostatika MeSH
• hyaluronan-iodine complex MeSH Prohlížeč
• jod MeSH
• jodidy MeSH
• kyselina hyaluronová MeSH
• Lugol's solution MeSH Prohlížeč