Activities of 19 enzymes were tested by the API ZYM system in 13 strains of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto, B. afzelii, B. garinii, B. lusitaniae, B. valaisiana) grown in liquid BSK-H medium supplemented with rabbit serum. All strains produced acid phosphatase, esterase (C4), esterase-lipase (C8), leucine arylamidase and naphthol-AS-BI-phosphohydrolase. Nine strains also produced alkaline phosphatase, and three strains produced alpha-glucosidase. The API ZYM system probably cannot be used for differentiation between B. burgdorferi sensu lato genomospecies.

• MeSH
• alfa-glukosidasy metabolismus   MeSH
• alkalická fosfatasa metabolismus   MeSH
• Borrelia burgdorferi komplex enzymologie   MeSH
• esterasy metabolismus   MeSH
• fosfatasy metabolismus   MeSH
• kyselá fosfatasa metabolismus   MeSH
• leucylaminopeptidasa metabolismus   MeSH
• lipasa metabolismus   MeSH
• lymeská nemoc mikrobiologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• alfa-glukosidasy MeSH
• alkalická fosfatasa MeSH
• esterasy MeSH
• fosfatasy MeSH
• kyselá fosfatasa MeSH
• leucylaminopeptidasa MeSH
• lipasa MeSH

A total of 2816 unfed adults nymphs of Ixodes ricinus ticks were collected from vegetation in Kosice (Eastern Slovakia) from 1994 to 1997. Prevalence of Borrelia burgdorferi s. l. in I. ricinus ticks, detected by dark field microscopy, varied and depended upon the year and the habitat of the collected ticks. The lowest prevalence was observed in 1994 (4.8%). During 1995 it increased to 17.2% and during the next two years decreased to 15.5% and 14.2%. The rate of infection varied from 2.1 to 23.3% within 10 examined habitats of the Kosice area. A different value of relative density of ticks was observed in various habitats. It ranged from 9-212 ticks per collecting hour within one flagged area (600 m2) which is 1.5-35.5 ticks per 100 m2. Eight isolates were obtained from the infected ticks. Electrophoresis and immunoblotting with 6 monoclonal antibodies were used for the identification of Borrelia strains. Three tick isolates were identified as B. burgdorferi s. s. and the other three isolates were found to be B. garinii. One strain reacted as a mixed culture of B. burgdorferi s. s., and B. garinii. The strain originated from the Vihorlat Mountains habitat and was detected by PCR-SSCP as B. burgdorferi s. s. with a small amount of B. afzelii. The obtained results emphasize the epidemiological importance not only of B. garinii and B. afzelii but also of B. burgdorferi s. s. in Central Europe.

• MeSH
• Borrelia burgdorferi komplex izolace a purifikace   MeSH
• Borrelia burgdorferi * MeSH
• Borrelia klasifikace   izolace a purifikace   MeSH
• elektroforéza MeSH
• klíště parazitologie   MeSH
• polymerázová řetězová reakce MeSH
• prevalence MeSH
• western blotting MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Slovenská republika MeSH

The survey is based on a total of 1263 records (738 isolations and 525 molecular DNA detections) of five Borrelia burgdorferi s.l. genomic groups available from 26 European countries: B. burgdorferi sensu stricto, B. afzelii, B. garinii, B. valaisiana (= VS116) and B. lusitaniae (= PoTiB2). It shows the geographic distribution, the source (ixodid ticks 802 records, fleas 2 records, mosquitoes 2 records, wild mammals 66 records, human patients 391 records) and the association of the genomic groups with particular clinical manifestations of Lyme borreliosis in humans (B. afzelii significantly prevails in skin lesions whereas B. garinii is more often associated with neuroborreliosis). The most frequent genomic groups in Europe are B. garinii (501 records) and B. afzelii (469 records). They occur across the continent and islands, whereas the third frequent genomic group, B. burgdorferi s.s. (201 records), has only rarely been isolated in eastern Europe. The remaining genomic groups, i.e. B. valaisiana (85 records) and B. lusitaniae (7 records) have only been isolated from, or detected in, Ixodes ricinus ticks in a few European countries.

• MeSH
• bakteriální nemoci kůže mikrobiologie   MeSH
• Borrelia burgdorferi komplex klasifikace   genetika   izolace a purifikace   MeSH
• Culicidae mikrobiologie   MeSH
• DNA bakterií analýza   MeSH
• genom bakteriální * MeSH
• klíšťata mikrobiologie   MeSH
• lidé MeSH
• lymeská nemoc mikrobiologie   MeSH
• nemoci periferního nervového systému mikrobiologie   MeSH
• savci mikrobiologie   MeSH
• Siphonaptera mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Evropa MeSH
• východní Evropa MeSH
• Názvy látek
• DNA bakterií MeSH