The fecal short-chain fatty acids concentration was higher (154 +/- 46.9 mmol/L) in childhood patients than in healthy children (96.6 +/- 19.2 mmol/L). On the other hand, pH values were nonsignificantly lower in patients stool (6.78 +/- 0.75 vs. children 7.42 +/- 0.74). Using denaturing gradient gel electrophoresis specific for total bacteria, lactobacilli and bifidobacteria the microbial population was characterized in fecal samples and in duodenal biopsies. Bacteria adhering to duodenal biopsies were not dominating in stool samples. More than 50 % of detected bacterial species belonged to as yet uncultured strains.

• MeSH
• Bacteria classification   genetics   isolation & purification   MeSH
• Biopsy MeSH
• Celiac Disease microbiology   MeSH
• Child MeSH
• DNA, Bacterial analysis   isolation & purification   MeSH
• Duodenum microbiology   MeSH
• Electrophoresis, Polyacrylamide Gel MeSH
• Feces chemistry   microbiology   MeSH
• Infant MeSH
• Hydrogen-Ion Concentration MeSH
• Fatty Acids, Volatile analysis   MeSH
• Humans MeSH
• Adolescent MeSH
• Polymerase Chain Reaction methods   MeSH
• Child, Preschool MeSH
• Intestines microbiology   MeSH
• Check Tag
• Child MeSH
• Infant MeSH
• Humans MeSH
• Adolescent MeSH
• Child, Preschool MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• DNA, Bacterial MeSH
• Fatty Acids, Volatile MeSH

The effect of gluten-free diet (GFD) and chitosan was evaluated in healthy individuals; GFD remarkably influenced the structure of the gut bacterial population and its metabolism. Administration of GFD and chitosan (3 g daily) significantly changed composition and metabolism of the bacterial population. Chitosan stimulated the counts of fecal chitinolytic bacteria and decreased the body mass of treated persons.

• MeSH
• Chitosan metabolism   pharmacology   MeSH
• Diet Therapy * MeSH
• DNA, Bacterial analysis   MeSH
• DNA Fingerprinting MeSH
• Adult MeSH
• Feces chemistry   microbiology   MeSH
• Glutens * MeSH
• Colon microbiology   MeSH
• Middle Aged MeSH
• Humans MeSH
• Check Tag
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Male MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Clinical Trial MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Chitosan MeSH
• DNA, Bacterial MeSH
• Glutens * MeSH

Growth of 6 bacterial strains representing dominant members of the human colonic microflora was measured in the presence of 0.025, 0.05 and 0.5 % chitosan (from shrimp shells, with a 97 % final degree of deacetylation). The effect of chitosan was variable and dependent on bacterial species. The most susceptible to chitosan were bacteria belonging to genera Bacteroides and Clostridium (91-97% growth inhibition). On the other hand, Roseburia sp., Eubacterium sp. and Faecalibacterium sp. were more resistant (63-83 % inhibition of growth). Chitosan can thus be considered as one of the means for influencing the bacterial population in the human colon.

• MeSH
• Bacteria, Anaerobic drug effects   growth & development   isolation & purification   MeSH
• Anti-Bacterial Agents pharmacology   MeSH
• Chitosan metabolism   pharmacology   MeSH
• Colon microbiology   MeSH
• Humans MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH
• Chitosan MeSH