Selected cerebrospinal-fluid (CSF) parameters (intrathecal synthesis of Borrelia-specific antibodies, oligoclonal IgG bands, CSF-to-serum quotient of albumin as a marker of blood-CSF barrier function and cytology) and typical CSF profile in neuroborreliosis were evaluated with the aim of elucidating possible clinical and laboratory similarities of neuroborreliosis (NB) and other neurological diseases (OND). From the cohort of 58 patients (38 diagnosed for NB, 20 with OND) NB patients had positive Borrelia-specific IgG antibodies in 97 % and positive Borrelia-specific IgM antibodies in 55 %; oligoclonal IgG bands were detected in 55%. The blood-CSF barrier was impaired in 89%, positive cytology was detected in 97% of the NB patients. Evaluation of specific intrathecal synthesis improves CSF diagnosis of NB, therefore, a combined CSF analysis has to be considered along with the clinical picture and medical history when formulating the diagnosis of NB.

• MeSH
• Borrelia burgdorferi komplex * imunologie   MeSH
• diferenciální diagnóza MeSH
• hematoencefalická bariéra MeSH
• histocytochemie MeSH
• imunoglobulin G mozkomíšní mok   MeSH
• imunoglobulin M mozkomíšní mok   MeSH
• lidé MeSH
• lymská neuroborelióza mozkomíšní mok   diagnóza   MeSH
• mozkomíšní mok * chemie   cytologie   imunologie   MeSH
• nemoci nervového systému mozkomíšní mok   diagnóza   MeSH
• protilátky bakteriální mozkomíšní mok   MeSH
• sérový albumin mozkomíšní mok   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• imunoglobulin G MeSH
• imunoglobulin M MeSH
• protilátky bakteriální MeSH
• sérový albumin MeSH

We have screened 91 migratory birds representing 32 species during the autumn of 2003 for the presence of the zoonotic pathogens Borrelia and Chlamydophila. Using polymerase chain reaction (PCR), B. burgdorferi sensu stricto was detected in cloacal swabs and, in two causes, also in throat swabs in 8 individuals (8.7 %) representing 7 birds species; B. garinii and B. afzelii were not detected. C. psittaci was detected only in cloacal swabs; 6 birds (6.6 %) from four species were found to be positive. The PCR products were sequenced and the sequences were compared phylogenetically with the gene sequences of 14 Chlamydophila strains retrieved from nucleotide databases; although the sequenced DNA was only 110 bp long, all obtained sequences created a new cluster with sublines branching from a position close to the periphery of the genus. All tested samples appear distinct within the known species and were most similar to C. felis or C. abortis.

• MeSH
• Borrelia burgdorferi klasifikace   genetika   izolace a purifikace   MeSH
• Chlamydophila psittaci klasifikace   genetika   izolace a purifikace   MeSH
• DNA bakterií analýza   chemie   genetika   MeSH
• farynx mikrobiologie   MeSH
• fylogeneze MeSH
• kloaka mikrobiologie   MeSH
• lymeská nemoc epidemiologie   mikrobiologie   veterinární   MeSH
• nemoci ptáků epidemiologie   mikrobiologie   MeSH
• polymerázová řetězová reakce MeSH
• psitakóza epidemiologie   mikrobiologie   veterinární   MeSH
• ptáci mikrobiologie   MeSH
• ribozomální DNA chemie   genetika   MeSH
• RNA ribozomální 16S genetika   MeSH
• sekvenční homologie nukleových kyselin MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Slovenská republika epidemiologie   MeSH
• Názvy látek
• DNA bakterií MeSH
• ribozomální DNA MeSH
• RNA ribozomální 16S MeSH

A simple assay by polymerase chain reaction was used for the of detection of Borrelia burgdorferi, causative agent of Lyme borreliosis (LB). It involves no DNA purification and is based on the amplification of a specific region of ospA gene of B. burgdorferi, followed by direct detection of the PCR product with SYBR Green I by agarose gel electrophoresis. The method was used to analyze samples from patients with LB diagnosis, with presumable infection with the LB spirochete, those with unclear clinical symptoms and after the course of an antibiotic treatment. Spirochetal DNA was detected by PCR even in contaminated samples in which B. burgdorferi was overgrown by fungi and other bacteria. Spirochetal DNA was detected and borrelia species was identified in cerebrospinal fluid of two patients hospitalized with the diagnosis "fever of unknown origin". Western blot and ELISA were negative in both cases. Total analysis of 94 samples from the hospital in Ceské Budejovice (South Bohemia, Czechia) showed infection with B. burgdorferi sensu stricto in 11% and B. garinii in 15% of cases. The highest prevalence was found for B. afzelii (43%). Co-infection was confirmed in 24 % of the analyzed symplex; 7% of samples that were B. burgdorferi sensu lato positive gave no results in DNA amplification with B. burgdorferi sensu stricto-, B. garinii- and B. afzelii-specific primers. The proposed reliable, rapid, unexpensive and specific technique could form the basis of laboratory tests for routine detection and identification of Lyme-disease spirochete in different samples.

• MeSH
• antigeny povrchové genetika   MeSH
• bakteriální vakcíny MeSH
• benzothiazoly MeSH
• Borrelia burgdorferi komplex klasifikace   genetika   imunologie   izolace a purifikace   MeSH
• chinoliny MeSH
• diaminy MeSH
• DNA bakterií analýza   izolace a purifikace   MeSH
• elektroforéza v agarovém gelu MeSH
• ELISA MeSH
• genotyp MeSH
• imunoglobulin G krev   MeSH
• imunoglobulin M krev   MeSH
• lidé MeSH
• lipoproteiny genetika   MeSH
• lymeská nemoc diagnóza   MeSH
• mozkomíšní mok mikrobiologie   MeSH
• organické látky metabolismus   MeSH
• polymerázová řetězová reakce metody   MeSH
• proteiny vnější bakteriální membrány genetika   MeSH
• protilátky bakteriální krev   MeSH
• senzitivita a specificita MeSH
• sérum mikrobiologie   MeSH
• techniky typizace bakterií MeSH
• western blotting MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny povrchové MeSH
• bakteriální vakcíny MeSH
• benzothiazoly MeSH
• chinoliny MeSH
• diaminy MeSH
• DNA bakterií MeSH
• imunoglobulin G MeSH
• imunoglobulin M MeSH
• lipoproteiny MeSH
• organické látky MeSH
• OspA protein MeSH Prohlížeč
• proteiny vnější bakteriální membrány MeSH
• protilátky bakteriální MeSH
• SYBR Green I MeSH Prohlížeč

Immunoelectrophoresis and its modifications were applied to analysis of a lipopolysaccharide-like component (LPS-LC) extracted from Borrelia garinii strains K24 and K48 isolated from Ixodes ricinus and Borrelia burgdorferi sensu stricto strain B31. A modification of the hot phenol-water method was used for isolation of LPS. Immunoelectrophoresis (IE) and crossed immunoelectrophoresis (CIE) of LPS-LC with polyclonal rabbit antisera revealed a pattern and properties partially similar to LPS from other Gram-negative bacteria. B. garinii LPS-LC formed in CIE a diffuse band extending from the start to the anode. Similarly, the shape and position of the band in IE did not show major differences from LPS of other Gram-negative bacteria. The LPS-LC extracted from the three genomic groups of B. burgdorferi sensu lato were found to have similar immunochemical properties irrespective of their genotype origin.

• MeSH
• Borrelia burgdorferi komplex chemie   genetika   imunologie   MeSH
• Borrelia burgdorferi chemie   imunologie   MeSH
• Borrelia chemie   imunologie   MeSH
• DNA bakterií genetika   MeSH
• druhová specificita MeSH
• imunoelektroforéza dvourozměrná MeSH
• imunoelektroforéza MeSH
• králíci MeSH
• lipopolysacharidy analýza   imunologie   MeSH
• protilátky bakteriální MeSH
• sekvence nukleotidů MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• DNA bakterií MeSH
• lipopolysacharidy MeSH
• protilátky bakteriální MeSH