Possible correlation between Toll-like receptor (TLR)-gene mutations and the susceptibility of the mammary gland to bacterial infections and also the associate breed-dependent aspects of somatic cell concentration (SCC), bacterial infection and TLR-gene mutations in sheep are described. In Polish Lowland Sheep (PLS), milk samples exceeding the level of 500/microL (i.e. 5 x 10(5) per mL) of SCC were recorded almost twice more frequently than in Polish Heath Sheep (PHS) (40 and 22.3%, respectively). The frequency of bacterial infections was also found in a similar ratio (20 and 12.7%, respectively). During detection of the TLR-gene mutation we recorded 2 alleles of TLR1, 6 alleles of TLR2 and 10 alleles of TLR4 genes in PHS sheep, while PLS sheep possessed 2, 4 and 6 alleles, respectively. Statistical analyses revealed a relationship between the specified TLR alleles, SCC and the frequency of incidence of bacterial inflammations of mammary gland. The data may serve as a benchmark for further study of TLR-gene mutation-dependent predisposition of mammary gland defensive cells to recognize the pathogen properly and initiate the immunological response, and may help in identifying one of the markers of natural resistance against sheep mastitis.

• MeSH
• alely MeSH
• bakteriální infekce genetika   veterinární   MeSH
• frekvence genu MeSH
• genetická predispozice k nemoci * MeSH
• mléčné žlázy zvířat mikrobiologie   MeSH
• mléko cytologie   mikrobiologie   MeSH
• nemoci ovcí genetika   MeSH
• ovce MeSH
• polymorfismus genetický * MeSH
• toll-like receptory genetika   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• toll-like receptory MeSH

We describe bacteriophage therapy in the case of a healthcare worker whose gastrointestinal tract was colonized by methicillin-resistant Staphylococcus aureus (MRSA) with subsequent urinary tract infection caused by the same pathogen. Oral treatment with anti-MRSA phages resulted in eradication of the carrier status.

• MeSH
• bakteriofágy * MeSH
• dospělí MeSH
• lidé MeSH
• přenašečství prevence a kontrola   MeSH
• přenos infekce ze zdravotnického pracovníka na pacienta prevence a kontrola   MeSH
• rezistence na methicilin * MeSH
• stafylokokové infekce prevence a kontrola   terapie   virologie   MeSH
• Staphylococcus aureus účinky léků   virologie   MeSH
• zdravotní sestry MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• práce podpořená grantem MeSH

Temperate bacteriophages were induced in and released from isolates of Staphylococcus aureus and Streptococcus agalactiae using mitomycin C. Various specific indicator cultures were tested for providing clear plaques after phage infection. Specific lytic mixture of bacteriophages was prepared using the induced, modified and laboratory variants of phages. Under laboratory conditions, the mixture eliminated all isolates from the tested collection of microorganisms. The restriction barrier of some bacterial isolates to bacteriophage infection was overcome either by UV irradiation or in vitro modification of bacteriophage DNA with specific methyltransferases. Conjugative R plasmids, capable of replication in G+ and G- bacteria, were detected and isolated from S. aureus and S. agalactiae antibiotic-resistant strains.

• MeSH
• aktivace viru MeSH
• DNA virů genetika   izolace a purifikace   MeSH
• lidé MeSH
• mnohočetná bakteriální léková rezistence genetika   MeSH
• R-plasmidy genetika   MeSH
• stafylokokové bakteriofágy fyziologie   MeSH
• Staphylococcus aureus účinky léků   genetika   izolace a purifikace   virologie   MeSH
• Staphylococcus účinky léků   genetika   izolace a purifikace   virologie   MeSH
• techniky in vitro MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA virů MeSH

Group B streptococcal (GBS) gene encoding the putative lipoprotein and adherence factor ScaAB was cloned and expressed in E. coli. Recombinant ScaAB protein was isolated. Signal sequence of ScaAB was found to be cleaved in the E. coli host. ScaAB recombinant protein was immunogenic in mice and antibodies against this protein were discovered in mice sera after GBS infection. The perspectives of the use of ScaAB protein in GBS vaccine are discussed.

• MeSH
• bakteriální adheze MeSH
• bakteriální proteiny chemie   genetika   imunologie   metabolismus   MeSH
• imunizace MeSH
• membránové proteiny chemie   genetika   imunologie   metabolismus   MeSH
• molekulární sekvence - údaje MeSH
• myši MeSH
• protilátky bakteriální krev   MeSH
• rekombinantní proteiny chemie   genetika   imunologie   metabolismus   MeSH
• sekvence aminokyselin MeSH
• Streptococcus agalactiae imunologie   izolace a purifikace   patogenita   MeSH
• streptokokové infekce mikrobiologie   mortalita   prevence a kontrola   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• bakteriální proteiny MeSH
• membránové proteiny MeSH
• protilátky bakteriální MeSH
• rekombinantní proteiny MeSH