Epigallocatechin-3-gallate (EGCG) is the main compound of green tea with well-described antioxidant, anti-inflammatory, and tumor-suppressing properties. However, EGCG at high doses was reported to cause liver injury. In this study, we evaluated the effect of EGCG on primary culture of rat hepatocytes and on rat liver mitochondria in permeabilized hepatocytes. The 24-hour incubation with EGCG in concentrations of 10 μmol/L and higher led to signs of cellular injury and to a decrease in hepatocyte functions. The effect of EGCG on the formation of reactive oxygen species (ROS) was biphasic. While low doses of EGCG decreased ROS production, the highest tested dose induced a significant increase in ROS formation. Furthermore, we observed a decline in mitochondrial membrane potential in cells exposed to EGCG when compared to control cells. In permeabilized hepatocytes, EGCG caused damage of the outer mitochondrial membrane and an uncoupling of oxidative phosphorylation. EGCG in concentrations lower than 10 μmol/L was recognized as safe for hepatocytes in vitro.

• MeSH
• čaj chemie   metabolismus   MeSH
• fluorescenční mikroskopie MeSH
• hepatocyty cytologie   účinky léků   metabolismus   MeSH
• jaterní mitochondrie účinky léků   metabolismus   MeSH
• kaspasa 3 metabolismus   MeSH
• katechin analogy a deriváty   toxicita   MeSH
• krysa rodu Rattus MeSH
• kultivované buňky MeSH
• membránový potenciál mitochondrií účinky léků   MeSH
• oxidativní fosforylace účinky léků   MeSH
• potkani Wistar MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• TNF-alfa metabolismus   MeSH
• viabilita buněk účinky léků   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• čaj MeSH
• epigallocatechin gallate MeSH Prohlížeč
• kaspasa 3 MeSH
• katechin MeSH
• reaktivní formy kyslíku MeSH
• TNF-alfa MeSH

BACKGROUND: Two-thirds partial hepatectomy (PHx) is an established model for the study of liver regeneration after resection. This process is accompanied by oxidative stress. AIMS: In our study, we tested the effect of epigallocatechin gallate (EGCG), a green tea antioxidant, on the early phase of liver regeneration after PHx. METHODS: Male Wistar rats were divided into five groups: (I) laparotomy + water for intraperitoneal injections, (II) laparotomy + EGCG 50 mg/kg body weight, (III) PHx + water for injections, (IV) PHx + EGCG 20 mg/kg and (V) PHx + EGCG 50 mg/kg, for 3 consecutive days. The rats were killed 24 h after surgery. Biochemical analysis of rat sera was performed. Histological samples were stained with hematoxylin & eosin and bromodeoxyuridine (BrdU). In hepatectomized rats, we also measured plasma malondialdehyde, tissue malondialdehyde, glutathione and cytokines levels, the activity of caspases 3/7, expression of Nqo-1 and HO-1 genes at the mRNA level, and expression of p21, p-p27 and p-p53 genes at the protein level. RESULTS: We observed lower accumulation of BrdU in group V when compared to groups III and IV. The activity of caspases 3/7 and expression of p-p53 were lower in group V than in groups III and IV. Tissue levels of IL-6 were lower in group V when compared to group III. Significant differences were not noted in other parameters. CONCLUSIONS: Administration of EGCG did not stimulate early phase liver regeneration in rats after PHx. There was even lower DNA synthesis in the group treated with a high dose of EGCG.

• MeSH
• antioxidancia farmakologie   terapeutické užití   MeSH
• cytokiny genetika   metabolismus   MeSH
• hemoxygenasa-1 genetika   metabolismus   MeSH
• hepatektomie metody   MeSH
• kaspasy genetika   metabolismus   MeSH
• katechin analogy a deriváty   farmakologie   terapeutické užití   MeSH
• krysa rodu Rattus MeSH
• messenger RNA genetika   metabolismus   MeSH
• NAD(P)H dehydrogenasa (chinon) genetika   metabolismus   MeSH
• oxidační stres fyziologie   MeSH
• potkani Wistar MeSH
• proteiny buněčného cyklu genetika   metabolismus   MeSH
• regenerace jater účinky léků   MeSH
• regulace genové exprese účinky léků   fyziologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• cytokiny MeSH
• epigallocatechin gallate MeSH Prohlížeč
• hemoxygenasa-1 MeSH
• kaspasy MeSH
• katechin MeSH
• messenger RNA MeSH
• NAD(P)H dehydrogenasa (chinon) MeSH
• NQO1 protein, rat MeSH Prohlížeč
• proteiny buněčného cyklu MeSH