Calcofluor-allied optical brightener Rylux BSU stimulated spore germination rate in Trichophyton mentagrophytes and Aspergillus fumigatus both if supplemented into Sabouraud glucose agar and if used for pretreatment of spore suspension prior to inoculation at low concentrations. Maximum stimulation of germination was obtained if 0.2% Rylux BSU was used for pretreatment in aqueous solution for 1 d prior to inoculation (130% in T. mentagrophytes and 150% in A. fumigatus, respectively). Pretreatment with 1% Rylux BSU provided strong protection against UV-irradiation and resulted in increased yields of cultural variants after UV-irradiation.

• MeSH
• Aspergillus fumigatus účinky léků   růst a vývoj   metabolismus   účinky záření   MeSH
• benzensulfonáty farmakologie   MeSH
• fluorescenční barviva MeSH
• kultivační média MeSH
• mikroskopie elektronová rastrovací MeSH
• spory hub růst a vývoj   MeSH
• Trichophyton účinky léků   růst a vývoj   metabolismus   účinky záření   MeSH
• ultrafialové záření MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• benzensulfonáty MeSH
• fluorescenční barviva MeSH
• kultivační média MeSH
• Rylux BSU MeSH Prohlížeč

Rylux BSU and congo red bind to chitin, interfere with proper cell-wall assembly, and stimulate chitin synthesis by increasing, most probably, chitin synthase 3 (ChS3) levels in Saccharomyces cerevisiae. On the other hand, the antibiotic nikkomycin Z inhibits chitin synthesis competitively. As ChS3 is the critical target of nikkomycin Z, its effect was tested in cells inhibited in growth by Rylux BSU or Congo red. Nikkomycin Z counteracted this inhibition but did not counteract aberrant cell-wall formation. These results indicate that chitin synthesis stimulation is the key step in Rylux BSU and congo red inhibition and support the idea that increase in chitin synthesis represents a compensatory response to damaged cell-wall structure. As Rylux BSU and congo red bind to newly synthesized chitin, further damage is caused in the wall and the response works in this case contrariwise. Nikkomycin Z breaks this vicious circle by counteracting the chitin synthesis stimulation.

• MeSH
• aminoglykosidy * MeSH
• antibakteriální látky farmakologie   MeSH
• barvicí látky farmakologie   MeSH
• benzensulfonáty farmakologie   MeSH
• buněčná stěna účinky léků   metabolismus   ultrastruktura   MeSH
• chitin biosyntéza   MeSH
• elektronová mikroskopie MeSH
• fluorescenční barviva farmakologie   MeSH
• Kongo červeň farmakologie   MeSH
• Saccharomyces cerevisiae účinky léků   růst a vývoj   metabolismus   ultrastruktura   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• aminoglykosidy * MeSH
• antibakteriální látky MeSH
• barvicí látky MeSH
• benzensulfonáty MeSH
• chitin MeSH
• fluorescenční barviva MeSH
• Kongo červeň MeSH
• nikkomycin MeSH Prohlížeč
• Rylux BSU MeSH Prohlížeč

The fluorescence brightener Rylux BSU (RBSU) showed an affinity for polysaccharide components of cell walls and accumulated in the extension zones of hyphal apices in Basidiobolus ranarum. It inhibited the polarized growth of mycelial hyphae and induced isotropic growth resulting in spherical thick-walled cells up to 456 microm in diameter. On the inner cell wall surface, massive protuberances were formed. The cell wall and protuberances were positive in PAS and the Grocott method and stained with fluorochromes Blankophor BA, Calcofluor, Uvitex 2B, Rylux BSU and FITC-labeled WGA- and ConA-lectins. The WGA-FITC fluorescence intensity of the wall's outermost layer, if not connected with neighbouring cells, and the fluorescence intensity of the innermost layer and of some protuberances mainly in their apical parts were on the average twice higher than the fluorescence intensity of the remaining wall material. RBSU binding to the cell wall material was stable. The process of converting from polarized to isotropic growth was reversible, depending upon contact with RBSU-containing medium. Repeated transfers of cells from RBSU-containing medium to an RBSU-free medium resulted in the development of apical swollen dumbbell-shaped cells.

• MeSH
• benzensulfonáty farmakologie   MeSH
• fluorescenční barviva farmakologie   MeSH
• houby cytologie   účinky léků   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• benzensulfonáty MeSH
• fluorescenční barviva MeSH
• Rylux BSU MeSH Prohlížeč