White rot fungi are well known for their ability to degrade xenobiotics in pure cultures but few studies focus on their performance under bacterial stress in real wastewaters. This study investigated mutual interactions in co-cultures of Pleurotus ostreatus and activated sludge microbes in batch reactors and different culture media. Under the bacterial stress an increase in the dye decolorization efficiency (95 vs. 77.1 %) and a 2-fold elevated laccase activity (156.7 vs. 78.4 Ul(-1)) were observed in fungal-bacterial cultures compared to pure P. ostreatus despite a limited growth of bacteria in mixed cultures. According to 16S-rDNA analyses, P. ostreatus was able to alter the structure of bacterial communities. In malt extract-glucose medium the fungus inhibited growth of planktonic bacteria and prevented shifts in bacterial utilization of potential C-sources. A model bacterium, Rhodococcus erythropolis responded to fungal metabolites by down regulation of uridylate kinase and acetyl-CoA synthetase.

• Klíčová slova
• Activated sludge, Batch reactor, Biodecolorization, Community structure, Pleurotus ostreatus, Uridylate kinase,
• MeSH
• aktivace enzymů MeSH
• Bacteria klasifikace   genetika   růst a vývoj   metabolismus   MeSH
• bioreaktory mikrobiologie   MeSH
• DNA bakterií analýza   MeSH
• down regulace MeSH
• fenotyp MeSH
• fungální proteiny analýza   MeSH
• genotyp MeSH
• koenzym A-ligasy metabolismus   MeSH
• lakasa metabolismus   MeSH
• mikrobiální interakce MeSH
• mikrobiální viabilita MeSH
• odpadní voda mikrobiologie   MeSH
• odpadní vody mikrobiologie   MeSH
• Pleurotus enzymologie   metabolismus   fyziologie   MeSH
• Rhodococcus enzymologie   růst a vývoj   metabolismus   MeSH
• ribozomální DNA analýza   MeSH
• RNA ribozomální 16S genetika   MeSH
• techniky vsádkové kultivace MeSH
• uhlík metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• acetate-CoA ligase (ADP-forming) MeSH Prohlížeč
• DNA bakterií MeSH
• fungální proteiny MeSH
• koenzym A-ligasy MeSH
• lakasa MeSH
• odpadní voda MeSH
• odpadní vody MeSH
• ribozomální DNA MeSH
• RNA ribozomální 16S MeSH
• uhlík MeSH

White-rot fungi that are efficient lignin degraders responsible for its turnover in nature have appeared twice in the center of biotechnological research - first, when the lignin degradation process started being systematically investigated and major enzyme activities and mechanisms involved were described, and second, when the huge remediation potential of these organisms was established. Originally, Phanerochaete chrysosporium became a model organism, characterized by a secondary metabolism regulatory pattern triggered by nutrient (mostly nitrogen) limitation. Last decade brought evidence of more varied regulatory patterns in white-rot fungi when ligninolytic enzymes were also abundantly synthesized under conditions of nitrogen sufficiency. Gradually, research was focused on other species, among them Irpex lacteus showing a remarkable pollutant toxicity resistance and biodegradation efficiency. Systematic research has built up knowledge of biochemistry and biotechnological applicability of this fungus, stressing the need to critically summarize and estimate these scattered data. The review attempts to evaluate the information on I. lacteus focusing on various enzyme activities and bioremediation of organopollutants in water and soil environments, with the aim of mediating this knowledge to a broader microbiological audience.

• MeSH
• Basidiomycota enzymologie   genetika   metabolismus   MeSH
• biodegradace MeSH
• biotechnologie * MeSH
• fungální proteiny genetika   metabolismus   MeSH
• látky znečišťující životní prostředí metabolismus   MeSH
• lignin metabolismus   MeSH
• regulace genové exprese u hub MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• fungální proteiny MeSH
• látky znečišťující životní prostředí MeSH
• lignin MeSH