Nejvíce citovaný článek - PubMed ID 17455800
Superantigen types in Staphylococcus aureus isolated from patients with cystic fibrosis
Three hundred twenty-one students (156 students with no clinical exposure and 165 students with clinical exposure) were screened for nasal colonization by Staphylococcus aureus; 20.9% of students were S. aureus nasal carriers, and 40.3% of S. aureus isolates harbored toxin genes. The most prevalent genes were tst (15.0 %) and sec (13.4 %). Isolates with multiple genes were only found among clinical students (p = 0.045). Six of 11 PFGE clones were positive for toxin genes. Methicillin-resistant (MRSA) isolates were only detected in the clinical students (4.5 %). The exposure of students to the hospital environment neither radically increased S. aureus nasal carriage, nor the frequency of clinically important toxin gene presence, but it could have influenced the positive selection of toxigenic MRSA strains.
- MeSH
- bakteriální léková rezistence MeSH
- bakteriální toxiny genetika MeSH
- enterotoxiny analýza MeSH
- exfoliatiny analýza MeSH
- exotoxiny MeSH
- leukocidiny MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- nos mikrobiologie MeSH
- přenašečství * MeSH
- Staphylococcus aureus účinky léků genetika izolace a purifikace MeSH
- studenti lékařství MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- bakteriální toxiny MeSH
- enterotoxiny MeSH
- exfoliatiny MeSH
- exotoxiny MeSH
- leukocidiny MeSH
- Panton-Valentine leukocidin MeSH Prohlížeč
Staphylococcal hospital isolates (n = 166) were tested in a touchdown multiplex-polymerase chain reaction assay for the identification of methicillin and mupirocin resistance and discrimination of S. aureus (femA gene) from coagulase negative staphylococci and other bacteria. All isolates harbored the 16SrDNA (Staphylococcus genus specific internal control) gene, and 130 (78 %) the mecA (methicillin resistance) gene. Fifty-seven (44 %) of these were determined as methicillin-resistant S. aureus, while the remaining 73 (56 %) were methicillin-resistant coagulase-negative staphylococci. Seventy-five (45 %) isolates harbored the ileS-2 (high-level mupirocin resistance) gene and were determined as mupirocin-resistant. This assay represents a simple, rapid, reliable approach for the detection and discrimination of methicillin-and mupirocin-resistant staphylococci.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální proteiny genetika metabolismus MeSH
- DNA bakterií genetika MeSH
- lidé MeSH
- methicilin farmakologie MeSH
- mnohočetná bakteriální léková rezistence * MeSH
- mupirocin farmakologie MeSH
- nemocnice MeSH
- polymerázová řetězová reakce metody MeSH
- rezistence na methicilin MeSH
- RNA ribozomální 16S genetika MeSH
- senzitivita a specificita MeSH
- stafylokokové infekce mikrobiologie MeSH
- Staphylococcus účinky léků genetika izolace a purifikace MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- Názvy látek
- antibakteriální látky MeSH
- bakteriální proteiny MeSH
- DNA bakterií MeSH
- methicilin MeSH
- mupirocin MeSH
- RNA ribozomální 16S MeSH
The occurrence of Staphylococcus aureus in rabbit feces, cecum and meat and its enterotoxin production, susceptibility to antibiotics and its sensitivity or resistance to bacteriocins produced by enterococci with probiotic properties were determined. Isolates were resistant to ampicillin, penicillin, phosphomycin and methicillin; a high percentage of susceptibility was also recorded to vancomycin, chloramphenicol, tetracycline and tobramycin. S. aureus isolates did not produce enterotoxins and were sensitive to partially purified enterocins (PPB) EK13, AL41 and EF2019 in the range of 100 to 12800 AU/mL; all S. aureus isolates, except the strain SA 2A/3, exhibited the highest sensitivity to PPB EK13. On the other hand, all strains were resistant to PPB CCM4231.
- MeSH
- bakteriociny metabolismus MeSH
- cékum mikrobiologie MeSH
- feces mikrobiologie MeSH
- hospodářská zvířata mikrobiologie MeSH
- králíci MeSH
- maso mikrobiologie MeSH
- mikrobiální testy citlivosti MeSH
- mnohočetná bakteriální léková rezistence * MeSH
- přemostěné cyklické sloučeniny metabolismus MeSH
- rezistence na methicilin * MeSH
- Staphylococcus aureus účinky léků izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Slovenská republika MeSH
- Názvy látek
- bakteriociny MeSH
- enterocin MeSH Prohlížeč
- přemostěné cyklické sloučeniny MeSH