Three hundred twenty-one students (156 students with no clinical exposure and 165 students with clinical exposure) were screened for nasal colonization by Staphylococcus aureus; 20.9% of students were S. aureus nasal carriers, and 40.3% of S. aureus isolates harbored toxin genes. The most prevalent genes were tst (15.0 %) and sec (13.4 %). Isolates with multiple genes were only found among clinical students (p = 0.045). Six of 11 PFGE clones were positive for toxin genes. Methicillin-resistant (MRSA) isolates were only detected in the clinical students (4.5 %). The exposure of students to the hospital environment neither radically increased S. aureus nasal carriage, nor the frequency of clinically important toxin gene presence, but it could have influenced the positive selection of toxigenic MRSA strains.

• MeSH
• Drug Resistance, Bacterial MeSH
• Bacterial Toxins genetics   MeSH
• Enterotoxins analysis   MeSH
• Exfoliatins analysis   MeSH
• Exotoxins MeSH
• Leukocidins MeSH
• Humans MeSH
• Microbial Sensitivity Tests MeSH
• Nose microbiology   MeSH
• Carrier State * MeSH
• Staphylococcus aureus drug effects   genetics   isolation & purification   MeSH
• Students, Medical MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Bacterial Toxins MeSH
• Enterotoxins MeSH
• Exfoliatins MeSH
• Exotoxins MeSH
• Leukocidins MeSH
• Panton-Valentine leukocidin MeSH Browser

Staphylococcal hospital isolates (n = 166) were tested in a touchdown multiplex-polymerase chain reaction assay for the identification of methicillin and mupirocin resistance and discrimination of S. aureus (femA gene) from coagulase negative staphylococci and other bacteria. All isolates harbored the 16SrDNA (Staphylococcus genus specific internal control) gene, and 130 (78 %) the mecA (methicillin resistance) gene. Fifty-seven (44 %) of these were determined as methicillin-resistant S. aureus, while the remaining 73 (56 %) were methicillin-resistant coagulase-negative staphylococci. Seventy-five (45 %) isolates harbored the ileS-2 (high-level mupirocin resistance) gene and were determined as mupirocin-resistant. This assay represents a simple, rapid, reliable approach for the detection and discrimination of methicillin-and mupirocin-resistant staphylococci.

• MeSH
• Anti-Bacterial Agents pharmacology   MeSH
• Bacterial Proteins genetics   metabolism   MeSH
• DNA, Bacterial genetics   MeSH
• Humans MeSH
• Methicillin pharmacology   MeSH
• Drug Resistance, Multiple, Bacterial * MeSH
• Mupirocin pharmacology   MeSH
• Hospitals MeSH
• Polymerase Chain Reaction methods   MeSH
• Methicillin Resistance MeSH
• RNA, Ribosomal, 16S genetics   MeSH
• Sensitivity and Specificity MeSH
• Staphylococcal Infections microbiology   MeSH
• Staphylococcus drug effects   genetics   isolation & purification   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Evaluation Study MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH
• Bacterial Proteins MeSH
• DNA, Bacterial MeSH
• Methicillin MeSH
• Mupirocin MeSH
• RNA, Ribosomal, 16S MeSH

The occurrence of Staphylococcus aureus in rabbit feces, cecum and meat and its enterotoxin production, susceptibility to antibiotics and its sensitivity or resistance to bacteriocins produced by enterococci with probiotic properties were determined. Isolates were resistant to ampicillin, penicillin, phosphomycin and methicillin; a high percentage of susceptibility was also recorded to vancomycin, chloramphenicol, tetracycline and tobramycin. S. aureus isolates did not produce enterotoxins and were sensitive to partially purified enterocins (PPB) EK13, AL41 and EF2019 in the range of 100 to 12800 AU/mL; all S. aureus isolates, except the strain SA 2A/3, exhibited the highest sensitivity to PPB EK13. On the other hand, all strains were resistant to PPB CCM4231.

• MeSH
• Bacteriocins metabolism   MeSH
• Cecum microbiology   MeSH
• Feces microbiology   MeSH
• Animals, Domestic microbiology   MeSH
• Rabbits MeSH
• Meat microbiology   MeSH
• Microbial Sensitivity Tests MeSH
• Drug Resistance, Multiple, Bacterial * MeSH
• Bridged-Ring Compounds metabolism   MeSH
• Methicillin Resistance * MeSH
• Staphylococcus aureus drug effects   isolation & purification   MeSH
• Animals MeSH
• Check Tag
• Rabbits MeSH
• Male MeSH
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Slovakia MeSH
• Names of Substances
• Bacteriocins MeSH
• enterocin MeSH Browser
• Bridged-Ring Compounds MeSH