We evaluated which of the fixatives and stains most frequently used for observation of insect tissues were the most appropriate for histopathological visualization of entomopathogenic fungal infections with Leptolegnia chapmanii in larvae of Aedes aegypti. The best contrast between the host tissues and the fungal structures was obtained when using a combination of Camoy fixative with Grocott staining contrasted with light green. Masson trichromic stain combined with 10% formaldehyde-phosphate buffer also provided satisfactory results--a good contrast and clearly distinguishable host tissues and fungal structures.

• MeSH
• Aedes růst a vývoj   mikrobiologie   MeSH
• chloroform MeSH
• ethanol MeSH
• fixativa MeSH
• histocytologické preparační techniky metody   MeSH
• kyselina octová MeSH
• larva mikrobiologie   MeSH
• oomycety patogenita   fyziologie   ultrastruktura   MeSH
• spory hub ultrastruktura   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• Názvy látek
• Carnoy's solution MeSH Prohlížeč
• chloroform MeSH
• ethanol MeSH
• fixativa MeSH
• kyselina octová MeSH